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Buying involving molecular drivers throughout child therapy-related myeloid neoplasms.
Rapid screening of pathogenic bacteria contaminated foods is crucial to prevent food poisoning. However, available methods for bacterial detection are still not ready for in-field screening because culture is time-consuming; PCR requires complex DNA extraction and ELISA lacks sensitivity. In this study, a microfluidic biosensor was developed for rapid, sensitive and automatic detection of Salmonella using metal-organic framework (MOF) NH2-MIL-101(Fe) with mimic peroxidase activity to amplify biological signal and Raspberry Pi with self-developed App to analyze color image. First, the target bacteria were separated and concentrated with the immune magnetic nanobeads (MNBs), and labeled with the immune MOFs to form MNB-Salmonella-MOF complexes. Then, the complexes were used to catalyze colorless o-phenylenediamine and H2O2 to produce yellow 2,3-diaminophenazine (DAP). Finally, the image of the catalysate was collected under the narrow-band blue light and analyzed using the Raspberry Pi App to determine the bacterial concentration. The experimental results showed that this biosensor was able to detect Salmonella Typhimurium from 1.5 × 101 to 1.5 × 107 CFU/mL in 1 h with the lower detection limit of 14 CFU/mL. The mean recovery for Salmonella in spiked chicken meats was ~112%. This biosensor integrating mixing, separation, labelling and detection onto a single microfluidic chip has demonstrated the merits of automatic operation, fast reaction, less reagent and small size, and is promising for in-field detection of foodborne bacteria.Two-dimensional (2D) nanomaterial-nucleic acid interactions have been widely used in the construction of fluorescent sensors, but they are rarely used in the construction of electrochemiluminescent (ECL) sensors and have never been used in the design of ratiometric ECL sensors. Therefore, a ratiometric ECL sensing platform was developed in this study based on the ECL resonance energy transfer (ECL-RET) of graphitic carbon nitride nanosheets (GCNNs)/Ru(bpy)32+ donor/acceptor pair. check details The 2D GCNNs showed much weaker affinity to the long dsDNA duplexes formed by hybridization chain reaction (HCR) than Ru(bpy)32+-lableled fuel hairpin DNAs (H1 and H2) for HCR. Therefore, the target-initiated HCR resulted in the luminescence enhancement of the GCNNs at 460 nm and the luminescence attenuation of the Ru(bpy)32+ at 610 nm. By measuring the I460 nm/I610 nm ratios, quantitative analysis of microRNA-133a was realized with a limit of detection of 0.41 pM. In addition, this ECL-RET sensing platform can be easily extended to detect metal ions or aptamer substrates by simply redesigning helper DNAs without changing the sequences of fuel hairpin DNAs. Moreover, due to the programmability of HCR, a series of sensitive logic gates ("OR", "INHIBIT", "AND", "NAND" and "INHIBIT-OR") based on the ECL-RET ratiometry can be constructed and responded to as low as 100 pM of Hg2+ or Ag+.Immunosensors are molecular recognition-based solid-state biosensing devices, in which the immunochemical reactions are coupled with transducers. As biologic or biochemical substances produced by tumor cells, tumor marker plays an important role in clinical diagnosis and treatment of cancer because its concentration is related to tumor size, clinical stage, and predicting prognosis. Voltammetric immunosensors based on the electrochemical analysis technique provide a sensitive electroanalytical approach for quantitatively detecting tumor markers by measuring the current as a function of the potential. To satisfy the need for accurate monitoring of tumor markers in low-concentration and their slight changes in concentration, the primary aim of developing a novel voltammetric immunosensor is to improve its sensitivity and limit of detection. Compared with traditional immunoassay, the advanced sensitivity-amplified immunosensors have applied appropriate amplification strategies to convert the bio-signal of antigen-antibody recognition events to the high electrochemical signal of redox species. Building on the significant concepts, sensitivity and limit of detection, we describe how the performance of voltammetric immunosensors can be improved by various sensitivity amplification mechanisms (1) construction of labels with a high loading of signal species; (2) introduction of interfacial reaction initiated by functionalized nanomaterials; (3) building a synergistic connection between labels and substrate. The review ends with a summary of the shortage of current sensitivity amplified immunosensors and the perspective of enhancement strategies for more simple, efficient, and reliable voltammetric immunosensors.The competitive endogenous RNA hypothesis is a new mechanism of RNA dialogue, in which circRNA-miRNA interaction (cmRRI) is found to be widely involved in the regulation of gene expression in tumors and other diseases. It is urgent but challenging to develop a convenient and efficient method to study the interaction between target circRNA and the candidate miRNAs. In this work, a biosensing method that allows directly analyzing cmRRI has been developed, so as to reveal the RNA dialogue strategy. The sensing system uses a bifunctional magnetic bead for the capture of target circRNA/miRNA complex as well as the signal amplification. Based on the nature of circRNA as a miRNA sponge, only if the target circRNAs and its regulatory miRNAs coexist as a complex, can the rolling circle amplification reaction be initiated to give a fluorescent signal as the output. Compared with traditional methods where the circRNA and its regulatory miRNAs have been separately analyzed, our design allows the integrated profiling of specific cmRRI by correlation characterization of two correlative RNAs, which represents a function-oriented method. The presented method also shows the analysis of the potential binding affinity of candidate miRNAs to target circRNAs. Furthermore, we have verified the ability of the sensor to directly detect cmRRI in biological samples, which reveals the promising applicability of this method for biomedical and clinical researches in the future.Recycling end-of-life tires (ELTs) reduces waste and provides a low-cost source of energy and materials such as crumb rubber, used as infill in artificial turf football pitches. However, some concerns were raised and remain about its safety. The potentially toxic human exposure to chemicals such as polycyclic aromatic hydrocarbons (PAHs), metals and others (volatile organic compounds (VOCs), plasticizers, antioxidants and additives) existing in ELTs (and in the resulting crumb rubber) is being studied, with no definitive conclusions. The literature existing so far suggests the possibility of their release from synthetic turf infill into the environment as water leachates and to the air surrounding the pitches, but there is the need of further research, also to assess the contribution of other materials present in synthetic turf. The database available comprised crumb rubber infill studies from pitches in 6 countries (USA, Norway, Netherlands, Portugal, Italy, Spain) and revealed a myriad of hazardous chemicals, with benzo[a]pyrene (n.
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