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Health-related Application of Elements Produced by Non-Pathogenic Fungi Aspergillus oryzae as well as a. luchuensis-Containing Koji.
Wheat is sensitive to heat stress, particularly during grain filling, and this reduces grain yield. Ancestral wheat species, such as emmer wheat (Triticum dicoccon Schrank), represent potential sources of new genetic diversity for traits that may impact wheat responses to heat stress. However, the diversity available in emmer wheat has only been explored superficially. Recently developed emmer derived hexaploid wheat genotypes were evaluated for physiological, phenological and agronomic traits in a multi-environment, multi-season strategy. this website The emmer-based hexaploid lines were developed from crosses and backcrosses to 9 hexaploid recurrent parents and these genotypes and 7 commercial cultivars were evaluated under two times of sowing (E1 and E2) in the field for three consecutive years (2014-2016). The materials were genotyped using a 90 K SNP platform and these data used to estimate the contribution of emmer wheat to the progeny. Significant phenotypic and genetic variation for traits were observed. Higher temperature during reproductive development and grain filling reduced trait expression. Emmer progeny with greater trait values than their recurrent parents and commercial cultivars in both environments were found. Derivatives with higher physiological trait values yielded well in both environments; as indicated by the clustering of genotypes. The emmer wheat parent contributed between 1 and 43 % of the genome of the emmer-based hexaploid progeny, and progeny with greater emmer contribution had superior trait values in both environments. These results showed a positive effect of direct emmer introgression on wheat performance under heat stress. Mitigation of high temperature stress through the introgression of favorable alleles from wheat close relatives into modern wheat cultivars is possible.Contemporary climate change is exposing plant populations to novel combinations of temperatures, drought stress, [CO2] and other abiotic and biotic conditions. These changes are rapidly disrupting the evolutionary dynamics of plants. Despite the multifactorial nature of climate change, most studies typically manipulate only one climatic factor. In this opinion piece, we explore how climate change factors interact with each other and with biotic pressures to alter evolutionary processes. We evaluate the ramifications of climate change across life history stages,and examine how mating system variation influences population persistence under rapid environmental change. Furthermore, we discuss how spatial and temporal mismatches between plants and their mutualists and antagonists could affect adaptive responses to climate change. For example, plant-virus interactions vary from highly pathogenic to mildly facilitative, and are partly mediated by temperature, moisture availability and [CO2]. Will host plants exposed to novel, stressful abiotic conditions be more susceptible to viral pathogens? Finally, we propose novel experimental approaches that could illuminate how plants will cope with unprecedented global change, such as resurrection studies combined with experimental evolution, genomics or epigenetics.Plant roots absorb K+ from soil via K+ channels and transporters, which are important for stress responses. In this research, GmAKT1, an AKT1-type K+ channel, was isolated and characterized. The expression of GmAKT1 was induced by K+-starvation and salinity stresses, and it was preferentially expressed in the soybean roots. And GmAKT1 was located in the plasma membrane. As an inward K+ channel, GmAKT1 participated in K+ uptake, as well as rescued the low-K+-sensitive phenotype of the yeast mutant and Arabidopsis akt1 mutant. Overexpression of GmAKT1 significantly improved the growth of plants and increased K+ concentration, leading to lower Na+/K+ ratios in transgenic Arabidopsis and chimeric soybean plants with transgenic hairy roots. In addition, GmAKT1 overexpression resulted in significant upregulation of these ion uptake-related genes, including GmSKOR, GmsSOS1, GmHKT1, and GmNHX1. Our findings suggested that GmAKT1 plays an important part in K+ uptake under low-K+ condition, and could maintain Na+/K+ homeostasis under salt stress in Arabidopsis and soybean plants.Alternative oxidase (AOX) is a mitochondrial enzyme encoded by a small nuclear gene family, which contains the two subfamilies, AOX1 and AOX2. In the present study on watermelon (Citrullus lanatus), only one ClAOX gene, belonging to AOX2 subfamily but having a similar gene structure to AtAOX1a, was found in the watermelon genome. The expression analysis suggested that ClAOX had the constitutive expression feature of AOX2 subfamily, but was cold inducible, which is normally considered an AOX1 subfamily feature. Moreover, one single nucleotide polymorphism (SNP) in ClAOX sequence, which led to the change from Lys (N) to Asn (K) in the 96th amino acids, was found among watermelon subspecies. Ectopic expression of two ClAOX alleles in the Arabidopsis aox1a knock-out mutant indicated that ClAOXK-expressing plants had stronger cold tolerance than aox1a mutant and ClAOXN-expressing plants. Our findings suggested watermelon genome contained a single ClAOX that possessed the expression features of both AOX1 and AOX2 subfamilies. A naturally existing SNP in ClAOX differentiated the cold tolerance of transgenic Arabidopsis plants, impling a possibility this gene might be a functional marker for stress-tolerance breeding.OVATE family proteins (OFPs) are plant-specific transcription factors that regulate plant growth and development. OFPs interact with 3-aa loop extension (TALE) homeodomain proteins and brassinosteroid (BR) signaling components to modulate gibberellic acid (GA) biosynthesis and BR responses. Bioactive GAs are essential in regulating plant organogenesis and organ growth by promoting cell differentiation and elongation. DELLA proteins act as the central repressors of GA-regulated processes and are targeted to be degraded by the 26S proteasome in the presence of GA. We discovered that the rice OFP22 negatively regulates GA and BR signal transduction. OsOFP22 expression was rapidly up-regulated by exogenous GA and BR application, detected predominantly in the calli and spikelets. Overexpression of OsOFP22 conferred multiple morphological phenotypes, including reduced plant height, dark green leaves, and shortened and widened leaves, floral organs and grains. The GA-induced elongation of the second leaf sheath in the seedlings, and α-amylase activity in the endosperms were attenuated in transgenic lines overexpressing OsOFP22, while GA-biosynthesis gene transcripts and bioactive GA3 and GA4 contents were increased in the transgenic plants.
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