Notes

Inbreeding despression symptoms contributes to the maintenance regarding habitat segregation involving closely connected monkeyflower types.
Microplastics (MP) are now considered ubiquitous across global aquatic environments. The ingestion of MP by fish and other marine vertebrates is well studied, but the ingestion of MP by marine invertebrates is not. Sponges (Phylum Porifera) are particularly understudied when it comes to MP ingestion, even though they are widely distributed across benthic habitats, can process large volumes of seawater, and can retain small particles within their water filtration systems. This study examines the presence of potential MP (PMP) in wild marine sponges and seawater collected in Bocas del Toro, Panamá. Subsurface seawater and tissue from six common Caribbean sponge species was collected in Saigon Bay, a heavily impacted, shallow-water coral reef. Seawater samples were filtered onto glass fiber filters to retain any PMP present and sponge tissue was digested with bleach, heated and filtered. Filters were examined using fluorescence microscopy to quantify PMP. An average of 107 ± 25 PMP L-1 was detected in seawater from Saigon Bay with particles ranging in size between 10 μm and ~3,000 μm. The number of PMP found in sponge tissue ranged between 6 ± 4 and 169 ± 71 PMP g-1 of dry tissue. Most particles found in sponge samples were very small (10-20 μm), but fibers greater than 5,000 μm were detected. Our results indicate that PMP exists within the tissues of the sponges we studied, but future studies should confirm the presence of MP in sponges using chemical analysis. Most importantly, the discrepancy between low levels of PMP in our sponge samples and high levels in the surrounding seawater highlights the potential for sponges to resist and/or egest MP. Finally, we provide a critical evaluation of our methods to improve their use in future MP work with benthic marine organisms.Light limitation is a major driver of succession and an important determinant of the performance of shade-intolerant tree seedlings. Shade intolerance may result from a resource allocation strategy characterized by rapid growth and high metabolic costs, which may make shade-intolerant species particularly sensitive to nutrient limitation and pathogen pressure. In this study, we evaluated the degree to which nitrogen availability and fungal pathogen pressure interact to influence plant performance across different light environments. To test this, we manipulated nitrogen availability (high, low) and access by foliar fungal pathogens (sprayed with fungicide, unsprayed) to seedlings of the shade-intolerant tree, Liquidambar styraciflua, growing at low and high light availability, from forest understory to adjacent old field. Foliar fungal damage varied with light and nitrogen availability; in low light, increasing nitrogen availability tripled foliar damage, suggesting that increased nutrient availability in low light makes plants more susceptible to disease. Despite higher foliar damage under low light, spraying fungicide to exclude pathogens promoted 14% greater plant height only under high light conditions. Thus, although nitrogen availability and pathogen pressure each influenced aspects of plant performance, these effects were context dependent and overwhelmed by light limitation. This suggests that failure of shade-intolerant species to invade closed-canopy forest can be explained by light limitation alone.The subfamily Goodeinae is a group of fishes endemic to the Mexican highlands. Most of the species are restricted to small and isolated streams or springs. Within this subfamily, the genus Characodon is the earliest diverging lineage of which three species have been described C. lateralis, C. audax, and C. garmani, with the latter, considered extinct. Characodon lateralis and C. audax are classed as endangered, and have been the subject of taxonomic controversy since their description previous studies have recognized a genetic differentiation in two groups separated by the El Salto waterfall, but morphological analyses contradict these genetic results. We perform a phylogeographic study using the mitochondrial cytb gene and d-loop region to elucidate the evolutionary history of C. lateralis and C. audax. The results with both markers show the presence of two highly differentiated haplogroups; one distributed north and the other distributed south of the waterfall, with genetic distances of 1.7 and 13.1% with cytb and d-loop respectively, and divergence calculated to have occurred 1.41 Mya. BOS172722 Significant genetic structure was found within each haplogroup and suggests the existence of at least four Evolutionary Significant Units (ESUs) within the examined populations. The possible processes identified as contributing to the formation of differentiated genetic groups are isolation, low population size, recurrent bottlenecks, and the strong sexual selection exhibited by the genus.The practice of conventional medicine is rooted in the ability of a patient to effectively communicate with their physician, and for the physician to comprehend the patient's story and perceive it through the lens of evidence-based practice. In reality, the differences in environmental backgrounds hinders this exchange of information and prevents a shared understanding and strategy. Narrative medicine provides a framework in which this divide can be bridged by encouraging the clinician to develop an appreciation of cultural nuances that drive a patient's decision making. The importance of this practice is highlighted with four stories in which the only path to competent patient management was through the utilization of narrative medicine.
The Health state descriptive system includes standardized self-administered instruments for measuring Health-Related Quality of Life (HRQoL) respectively among adolescents, and children. The objectives of the current study were (1) to translate and adapt the pediatric-adolescent version 16D and 17D from English into French (Canada), (2) to demonstrate their feasibility in pediatric conditions.

The translation methodology combined forward and back translations, and cognitive debriefing with eight adolescents and eight children. Four bilingual translators were involved in the process. We administered the translated versions to two clinical samples, being treated for Primary immunodeficiency (PID,
 = 48, aged 14.1 years, 20 girls), and having recovered from pediatric Acute Lymphoblastic Leukemia (ALL,
 = 153, aged 14.7 years, 77 girls).

Cognitive debriefing indicated that that the instructions, items, and response options were clear, easy to understand, and easy to answer. Adjustments were made for clarity.
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