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In times of crisis, people have historically had to band together to overcome. What happens when they cannot? This article examines the reality of people forced to isolate from one another during one of the most turbulent events of their lives the COVID-19 pandemic. Connecting the dots of topics including fear, social stigmas, global public response and previous disease outbreaks, this article discusses the negative mental health effects that individuals and communities will likely suffer as the result of social distancing, isolation and physical infection.Objectives Emergency preparedness becomes more important with increased age, as older adults are at heightened risk for harm from disasters. In this study, predictors of preparedness actions and confidence in preparedness among older adults in the United States were assessed. Methods This nationally representative survey polled community-dwelling older adults ages 50-80 y (n = 2256) about emergency preparedness and confidence in addressing different types of emergencies. Logistic regression was used to identify predictors of reported emergency preparedness actions and confidence in addressing emergencies. Results Participants' mean age was 62.4 y (SD = 8); 52% were female, and 71% were non-Hispanic white. Living alone was associated with lower odds of having a 7-d supply of food and water (adjusted odds ratio [aOR] = 0.74; 95% confidence interval [CI] 0.56-0.96), a stocked emergency kit (aOR = 0.64; 95% CI 0.47-0.86), and having had conversations with family or friends about evacuation plans (aOR = 0.59; 95% CI 0.44-0.78). Use of equipment requiring electricity was associated with less confidence in addressing a power outage lasting more than 24 h (aOR = 0.66; 95% CI 0.47-0.94), as was use of mobility aids (OR = 0.65; 95% CI 0.45-0.93). Conclusions These results point to the need for tailored interventions to support emergency preparedness for older adults, particularly among those who live alone and use medical equipment requiring electricity.Diet has direct and indirect effects on health through inflammation and the gut microbiome. We investigated total dietary inflammatory potential via the literature-derived index (DII®) with gut microbiota diversity, composition, and function. In cancer-free patient volunteers initially approached at colonoscopy and healthy volunteers recruited from the medical center community, we assessed 16S rDNA in all subjects who provided dietary assessments and stool samples (n=101) and the gut metagenome in a subset of patients with residual fasting blood samples (n=34). Associations of energy-adjusted DII scores with microbial diversity and composition were examined using linear regression, permutational multivariate analysis of variance, and linear discriminant analysis. Spearman correlation was used to evaluate associations of species and pathways with DII and circulating inflammatory markers. Alpha- and beta-diversity did not significantly differ across DII levels; however, Ruminococcus torques, Eubacterium nodatum, Acidaminococcus intestini, and Clostridium leptum were more abundant in the most pro-inflammatory diet group, while Akkermansia muciniphila was enriched in the most anti-inflammatory diet group. Selleck Nobiletin With adjustment for age and BMI, R. torques, E. nodatum, A. intestini remained significantly associated with a more pro-inflammatory diet. In the metagenomic and fasting blood subset, A. intestini was correlated with circulating PAI-1, a pro-inflammatory marker (rho=0.40), but no associations remained significant upon correction for multiple testing. An index reflecting overall inflammatory potential of the diet was associated with specific microbes, but not overall diversity of the gut microbiome in our study. Findings from this preliminary study warrant further research in larger samples and prospective cohorts.Scanning electron microscopy, coupled with energy-dispersive X-ray spectroscopy (EDS), is a powerful tool used in many scientific fields. It can provide nanoscale images, allowing size and morphology measurements, as well as provide information on the spatial distribution of elements in a sample. This study compares the capabilities of a traditional EDS detector with a recently developed annular EDS detector when analyzing electron transparent and beam-sensitive NaCl particles on a TEM grid. The optimal settings for single particle analysis are identified in order to minimize beam damage and optimize sample throughput via the choice of acceleration voltage, EDS acquisition time, and quantification model. Here, a linear combination of two models is used to bridge results for particle sizes, which are neither bulk nor sufficiently thin to assume electron transparent. Additionally, we show that the increased count rate obtainable with the annular detector enables mapping as a viable analysis strategy compared with feature detection methods, which only scan segmented regions. Finally, we discuss advantages and disadvantages of the two analysis strategies.Social isolation is a state of nearly-absolute lack of interaction between an individual and society. The Friendship Scale (Hawthorne, 2006) is a measure of social isolation that needed to be translated in the Urdu language for its validation for the Pakistani population owing to its brevity and sound psychometric properties. For the Urdu translation, the standard back-translation procedure was adopted, and the cross-language validation of the translated version was undertaken on a purposive sample of (N = 60) older adults with a minimum age of 60 years. The test-retest reliability of one week for the Urdu-English and English-Urdu version was .95 and .97, respectively. In an independent purposive sample of older adults (N = 500; men = 263 and women = 237) from Lahore and Sargodha districts, the CFA of the Friendship Scale revealed a single factor solution with six indicators, which demonstrated configural, metric, and scalar invariance across both genders and comparable latent mean scores of men and women. The Friendship Scale demonstrated a significant positive relationship with depression and non-significant association with the assimilation, which provided evidence for the convergent and discriminant validities, respectively. Furthermore, evidence of the concurrent validity was established as the older adults whose spouses had died scored significantly higher on the Friendship scale as compared to their counterparts who were living with their spouses. These pieces of evidence suggest that the Urdu version of the Friendship scale is a reliable and valid measure of flourishing for both genders.
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