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Surgical treatment regarding oligometastasis involving pancreatic cancers.
In this work, two different microfluidic paper-based analytical devices (μPADs) were developed for the quantification of nitrite and nitrate in human saliva samples, in order to aid in the diagnosis of some diseases and health conditions associated with these ions. The development of these nitrite and nitrate μPADs involved several studies to optimize their design and construction, including an interference assessment and stability studies. These μPADs allowed a nitrite determination in a range of 5-250 μM with limits of detection and quantification of 0.05 μM and 0.17 μM, respectively, and a nitrate determination in the range 0.2-1.2 mM with limits of detection and quantification of 0.08 mM and 0.27 mM, respectively. As for the stability, both of the μPADs were stable when stored in vacuum at 4 °C (the nitrite μPAD for at least 60 days and the nitrate μPAD for at least of 14 days) and, after the sample placement, the nitrite and nitrate μPADs could be scanned within the first 4 and 2 h, respectively. The nitrite μPAD measurements were compared with the ones obtained from the standard colorimetric method and there were no statistically significant differences between these two methods. To evaluate the accuracy of nitrate μPAD measurements, 4 certified water samples were used and recovery studies using saliva samples were performed.A novel dielectric barrier discharge (DBD) reactor was utilized to in situ enrich and atomize lead in gas phase. The structure of DBD reactor was optimized to broaden the acidity window of plumbane generation from 1% to 3.5%, bringing better analytical stability and practicability deriving from hydride generation process. For the first time DBD proved effective in lead preconcentration and broadening the acidity window of plumbane generation. Pb can be trapped quantitatively (~100%) on the quartz surface of DBD tube under O2-containing atmosphere and released (~100%) under H2-containing atmosphere. The absolute detection limit (LOD) for Pb was 4.1 pg (injection volume = 1.2 mL), and the linear (R2 > 0.999) range was 0.05-100 μg/L. The results were in good agreement with those of certified reference materials (CRMs), and spiked recoveries for surface water samples were 99-104% with 2-8% RSD. By gas phase analyte enrichment, the proposed method reduced absolute LOD by 10 times. It was deduced that plumbane was changed to lead oxide species trapped on the quartz tube surface and then released, and transported in form of atoms to the detection zone.Benefiting from their excellent optical absorption and electron transfer properties, manganese dioxide nanomaterials have been widely applied for fluorescence-based nanosensors. In this work, graphene quantum dots (GQDs) wrapped square-plate-like MnO2 nanocomposite was synthesized from potassium permanganate via an in situ redox procedure under ultrasonication with poly(allylamine hydrochloride) (PAH) being involved. Through an effective fluorescence resonance energy transfer (FRET) process and inner filter effect (IFE) between GQDs and MnO2, the fluorescence of GQDs was quenched. MMP-9-IN-1 molecular weight Furthermore, the introduction of glutathione (GSH) decomposed MnO2 and caused fluorescence recovery of GQDs. Therefore, a MnO2 mediated nanosensor was established for fluorescent turn-on sensing of GSH. A satisfactory linear range was found to be 0.07-70 μM and the detection limit was as low as 48 nM. Besides, the fluorescent recognition of cancer cells using GQDs-MnO2 nanocomposite was achieved because of the obviously higher GSH content in cancer microenvironment than normal cells. This nanosensor was constructed directly in GQDs solution in the presence of PAH without the complicated modifications or connections, making it a facile and novel nanosensor for GSH.Cis-diol compounds are class of biomolecules including nucleosides, glycoproteins, saccharides, and nucleotides, which play vital roles in various biological processes. Due to low abundances of these species in the complex biological samples, their identification and analysis is difficult. Boronate affinity materials are commonly used for the isolation and enrichment of cis-diol compounds, due to their unique, facile and selective enrichment mechanism. In this study we report a selective approach to extract nucleosides, glycopeptides and glycoproteins using boronic acid functionalized GMA-MAA-DVB polymer. This novel polymer, reported for the first time in proteomics, have high BET surface area (132.8447 m2 g-1) which contribute to efficient enrichment and average pore size (20.3449 nm) to facilitates the nano confinement effect for strong interactions. Hydrophilic character of methacrylic acid and diethylenetriamine, along with inherent affinity of boronic acid for glycosylated biomolecules result in selectivity up to 1500 for peptides and 11000 for glycoprotein. Binding constant for cis-diol compounds are in the range of 10-4 to 10-6 M and theoretical binding capacity up to 85 mg g-1 for HRP and 180 mg g-1 for IgG, respectively. Furthermore, boronic acid functionalized polymer (BFP) enrich glycoproteins and glycopeptides in range of 1 pg mL-1 and 0.04 ng mL-1 with S/N ≥ 3. Finally, material is applied to enrich the glycoproteins from healthy human saliva sample and six glycoproteins are identified.Headspace solid-phase microextraction (HS-SPME) of low volatile analytes from complex aqueous samples can be substantially facilitated by elevating the temperature of the samples. However, many SPME coatings prepared from novel sorptive materials may suffer from low stabilities in hot water steam. Herein, a superhydrophobic metal-organic framework (MOF) derived from decorating the metal-oxo nodes of the amino-functionalized UiO-66(Zr) with phenylsilane was prepared and successfully developed into a novel SPME fiber coating. The highest extraction efficiencies towards the semi-volatile ultraviolet (UV) filters were achieved when the aqueous samples were heated up to 100 °C. It was notable that the lab-made coating exhibited extraordinary stability towards hot water steam, probably because the hydrophobic groups capped on the MOF prevented water molecules from entering and deconstructing its lattice. Even after being treated with water steam under 100 °C for 21 h, the extraction performance of the coating remained unchanged, and the crystal structure of the MOF maintained.
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