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Purification of diverse hemoglobins simply by metallic sea precipitation.
Our analysis provides new clues for a better understanding of the pathogenic mechanisms and offers potential therapeutic targets for osteoporosis. Copyright © 2020 Dong, Zhou, Wang, Zuo, Ying, Chai, Fei, Jin, Chen, Ma and Liu.In the aging western population, the average age of incidence for spinal cord injury (SCI) has increased, as has the length of survival of SCI patients. This places great importance on understanding SCI in middle-aged and aging patients. Axon regeneration after injury is an area of study that has received substantial attention and made important experimental progress, however, our understanding of how aging affects this process, and any therapeutic effort to modulate repair, is incomplete. The growth and regeneration of axons is mediated by both neuron intrinsic and extrinsic factors. In this review we explore some of the key extrinsic influences on axon regeneration in the literature, focusing on inflammation and astrogliosis, other cellular responses, components of the extracellular matrix, and myelin proteins. We will describe how each element supports the contention that axonal growth after injury in the central nervous system shows an age-dependent decline, and how this may affect outcomes after a SCI. Copyright © 2020 Sutherland and Geoffroy.The hair follicle undergoes a regular cycle composed of three phases anagen, catagen, and telogen. selleckchem The life of follicular melanocytes is totally linked to the hair cycle; and during anagen or the growth phase, the melanocytes are active and produce the melanin responsible of hair shaft pigmentation. Various signaling pathways regulate the hair growth cycle and, therefore, the pigmentation; we distinguish the Wnt/β-catenin signaling pathway as it plays a major role in the development, growth, and proliferation of the melanocytes and the activation of melanogenesis enzymes and the related transcription factor. In this study, 3,4,5-tri-O-caffeoylquinic acid (TCQA), a caffeoylquinic acid derivative, stimulated the pigmentation in C3H mouse hair follicle, in human melanocytes, and B16F10 melanoma cells. An enhancement in pigmentation associated genes was observed upon TCQA treatment in vivo and in vitro. Interestingly, the expression of β-catenin was remarkably upregulated in mouse treated skin and in pigment cell lines. Moreover, TCQA upregulated CTNNB1 expression after inhibition in human melanocytes. Taken together, this study suggests that TCQA triggered β-catenin activation to enhance the pigmentation during the anagen phase of the hair cycle. Copyright © 2020 Bejaoui, Villareal and Isoda.Androgenetic alopecia (AGA) is the most common type of hair loss, and is mainly caused by the biological effects of testosterone on dermal papilla cells (DPCs). In vitro culturing of DPCs might be a useful tool for the screening of target molecule of AGA. However, primary DPCs cannot continuously proliferate owing to cellular senescence and cell culture stress. In this study, we introduced mutant cyclin-dependent kinase 4 (CDK4), Cyclin D1, and telomerase reverse transcriptase (TERT) into DPCs. We confirmed protein expression of CDK4 and Cyclin D1, and enzymatic activity of TERT. Furthermore, we found the established cell line was free from cellular senescence. We also introduced the androgen receptor gene using a recombinant retrovirus, to compensate the transcriptional suppressed endogenous androgen receptor in the process of cell proliferation. Furthermore, we detected the efficient nuclear translocation of androgen receptor into the nucleus after the treatment of dihydrotestosterone, indicating the functionality of our introduced receptor. Our established cell line is a useful tool to identify the downstream signaling pathway, which activated by the testosterone. Copyright © 2020 Fukuda, Takahashi, Takase, Orimoto, Eitsuka, Nakagawa and Kiyono.Trichoderma harzianum is a biological control agent used against phytopathogens and biostimulation in agriculture. However, its efficacy can be affected by biotic and abiotic factors, and microencapsulation has been used to maximize the efficacy. The objective was to develop polymeric microparticles to encapsulate T. harzianum, to perform physicochemical characterization to evaluate its stability, to evaluate effects on the soil microbiota, antifungal activity in vitro and enzymatic activity. Size distribution of wet and dry microparticles was 2000 and 800 μm, respectively. Scanning electron microscopy showed spherical morphology and encapsulation of T. harzianum. Photostability assays showed that encapsulation protected the fungus against ultraviolet radiation. The evaluation of the microbiota showed that the proportion of denitrifying bacteria increased when compared to the control. The T. harzianum encapsulation showed an improvement in the chitinolytic and cellulosic activity. In vitro tests showed that encapsulated fungus were able to provide a greater control of S. sclerotiorum. Copyright © 2020 Maruyama, Bilesky-José, de Lima and Fraceto.Tarsal plate regeneration has always been a challenge in the treatment of eyelid defects. The commonly used clinical treatments such as hard palate mucosa grafts cannot achieve satisfactory repair effects. Tissue engineering has been considered as a promising technology. However, tarsal plate tissue engineering is difficult to achieve due to its complex structure and lipid secretion function. Three-dimensional (3D) printing technology has played a revolutionary role in tissue engineering because it can fabricate complex scaffolds through computer aided design (CAD). In this study, it was novel in applying 3D printing technology to the fabrication of tarsal plate scaffolds using poly-caprolactone (PCL). The decellularized matrix of adipose-derived mesenchymal stromal cells (DMA) was coated on the surface of the scaffold, and its biofunction was further studied. Immortalized human SZ95 sebocytes were seeded on the scaffolds so that neutral lipids were secreted for replacing meibocytes. In vitro experiments revealed excellent biocompatibility of DMA-PCL scaffolds with sebocytes. In vivo experiments revealed excellent sebocytes proliferation on the DMA-PCL scaffolds. Meanwhile, sebocytes seeded on the scaffolds secreted abundant neutral lipid in vitro and in vivo. In conclusion, a 3D-printed PCL scaffold modified with DMA was found to be a promising substitute for tarsal plate tissue engineering. Copyright © 2020 Chen, Yan, Wu, Zhang, Yan, Yao, Sun and Fu.
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