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95 pg TEQ/kg bw/day; contributions from dlPCBs, PXDD/Fs and PBDD/Fs resulted in an average estimated daily intake of 6.56 pg TEQ/kg bw/day. The results obtained in this study, although lower than infant dietary intake estimates in human milk from industrialized countries, exceeded the recommended safety standards of 1 pg TEQ/kg bw/day and 1-4 pg TEQ/kg bw/day from the Agency for Toxic Substances and Disease Registry (ATSDR) and the World Health Organization (WHO), respectively.Subsurface storm flow of phosphorus (P), including particulate P, has been recently discussed as an important P transport path in contrast to typical surface runoff events. However, P speciation, and P concentration during storm events has not been extensively investigated; therefore, its contribution to the water quality is not clearly understood. In this study, the physicochemical properties of particulate P in tile water samples during a high flow event were investigated in Midwestern agricultural lands using wet chemical methods, 31P Nuclear Magnetic Resonance spectroscopy and P K-edge X-ray absorptions near edge structure spectroscopy. In slightly alkaline pH tile water, total P was ranging from ∼0.06 to 0.22 mg L-1, which is significantly greater than dissolved reactive P (DRP) (∼0.02-0.08 mg L-1). The tile water contains P enriched particulate matters (∼200-660 mg L-1). Total P in the colloidal fraction was from 1013 to 2270 mg kg-1. Phosphate and organic P species, especially monoesters, are sorbed in soil colloids like calcite, and iron oxides, and colloids are effective carriers of P in the subsurface transport process during storm events. CP-690550 The results of this study show that storm events can accelerate the subsurface transport of P with soil particles in addition to DRP.Despite the North American production ban of polychlorinated biphenyls (PCBs), PCBs are ubiquitous in the environment and in wildlife tissues. Chelydra serpentina serpentina (common snapping turtle) have been used as environmental indicators of PCB pollution upwards of 40 years given their high site fidelity and high trophic position. Despite their long use as indicators of PCB contamination, the effects of PCBs in reptiles remain largely unknown. In this study, we performed two experiments to assess i) bioaccumulation and ii) toxicity of PCBs to 1-month-old C. s. serpentina, to aid in interpretation of PCB burdens. Food pellets were spiked at an environmentally relevant concentration (0.45 μg/g) of the PCB mixture Aroclor 1254 to model hepatic bioaccumulation and depuration, through feeding, for 31 days and clean food for 50 days, respectively. No significant differences in PCB concentrations were observed in liver tissue over the course of the experiment, suggesting that juvenile turtles can likely metabolize low environmentally occurring concentrations of PCBs. Additionally, a dose-response experiment, performed to determine hepatic toxicity and bioaccumulation in juvenile C. s. serpentina, showed a 1.8-fold increase in hepatic expression of cyp1a when fed A1254-spiked pellets (12.7 μg/g; range 0-12.7 μg/g). This gene induction correlates with the significant increase of group 3 PCB congeners measured in the turtle liver, which are known to be metabolized by CYP1A. This study indicates that C. s. serpentina may be a good environmental indicator for PCBs, while more research is needed to assess the effects of body burdens in wild C. s. serpentina.This publication presents a comparison of the content of pollutants in groundwater samples taken at 117 measurement points in four regions of Poland during a drought period and in the reference period without drought. Based on the chemical analyses of water, an assessment of the health risk resulting from the use of underground water for consumption was carried out. The study aimed to determine whether drought affects the increase in health risk exposure of the population. It was found that despite the occurrence of drought, the expected increase in the concentration of pollutants in water does not take place in all locations. This study found that in some cases the occurrence of drought did not cause an increase in the non-cancerogenic threat expressed by the hazard index. There were also no clear changes in excess lifetime cancer risk values except for selected measurement points. On the other hand, the statistical analysis of all data collected in the regions where the research was conducted showed a general trend of increasing environmental health risk caused by changes in groundwater pollution during drought.This paper illustrates the development of a miniaturized and precise analytical tool for biomonitoring of honey bee exposure to insecticides. This is the first work describing an analytical method for determination of very low concentrations of a wide range of insecticides in maize guttation fluid. Seed treatment with systemic insecticides or their foliar application causes the accumulation of compounds in the guttation liquid, which consists of excess water and compounds removed by plants and is a source of water for bees. A micro-QuEChERS protocol using 1 g of sample was used for analysis of over 140 insecticides belonging to 30 different chemical classes by LC-ESI-MS/MS. The determination of insecticides in guttation fluid is a difficult analytical task due to 1) the complexity of the sample matrix, 2) small amounts of test samples and 3) trace levels of analytes (often equal sublethal dose of insecticide for bees). An efficient sample treatment is proposed, involving 1 g of sample, extraction with 1% formic acid in acetonitrile, frozen, ultrasound-assisted, centrifugation and dispersive solid phase extraction with nano graphene oxide. Other tested sorbents Fe3O4MNPs and two mixtures PSA/C18/GCB and Z-Sep did not give satisfactory parameters during sample purification. The graphene oxide proved to be the best, ensuring negligible matrix effects and analyte recoveries between 70% and 120% with relative standard deviations less then 20% for most of the compounds studied. The proposed method enables assessment of risk to honey bees resulting from exposure to guttation fluids containing toxic insecticides at very low concentrations.
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