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Duplication associated with Chromosome 16p13.11-p12.3 with Different Words and phrases within the Same Household.
Surface-enhanced Raman spectroscopy (SERS) became a useful analytical technique with the development of appropriate metallic substrates. The need for SERS substrates that immobilize metallic nanoparticles prompted this work to search for an appropriate material. This work presents the preparation, characterization and application of a SERS substrate for crystal violet (CV) detection, as the probe molecule. The inner layer of the substrate is a thin film of the fungal β-D-glucan, botryosphaeran, covered by a thin layer of silver nanoparticles (AgNPs). The nanoparticles were produced by laser ablation, a fast and clean method for their preparation, and the layers were assembled by casting. Scanning electron and atomic force microscopies, UV-VIS and Raman spectroscopy and X-ray diffraction allowed the characterization of the surface of the substrate. Analysis by Raman spectroscopy showed promising results for SERS amplification on the substrate. Detection of CV reached enhancement factors up to 106 orders of magnitude, compared to normal Raman spectra. Linearity was observed for analyses on the SERS substrate at concentration ranges of 0.005 to 1 µmol L-1. The assembly reached the detection of 12 pmol cm-2 of CV, which corresponds to 96 fg of the probe molecule contained in the area of the substrate effectively interacting with the laser. The substrate was more efficient than silver colloids to perform SERS.A smartphone-combined dual-emission ratiometric fluorescence probe for specifically and visibly detecting cephalexin was first designed. In the probe, blue-emitting fluorescent carbon dots (CDs) was synthesized and covered with a layer of silica spacer. Red-emitting fluorescent CdTe QDs (r-QDs) was grafted onto the silica nanospheres as an analytical probe. Then, the cephalexin antibody was covalent grafted to the ratio sensor to increase the selectivity. The ratio of fluorescence intensity (FL) of r-QDs and CDs was quenched with the increasing concentration of cephalexin. The detection method has good linear response in the range of 1-500 μM and the detection limit was 0.7 μM. Then portable device based on smartphone detection was constructed according to the color change under UV lamp. The detection image was obtained through the smartphone camera, and the color picker APP installed in the smartphone captured the RGB value of the image. In addition, this method was also used to determine the amount of cephalexin in milk samples with recovery of 94.1%-102.2%. These results showed that it was a portable, simple and visible method to detect cephalexin in food analysis and environmental monitoring.In the present work, four simple and economic spectrophotometric methods, namely constant center (CC), ratio difference (RD), H-point standard addition method (HPSAM), and ratio H-point standard addition method (RHPSAM) were employed for the simultaneous determination of meta and para-nitroaniline with severely overlapping spectra without unitizing the preliminary physical separation techniques. In each method, the parameters affecting the resolution of the spectra were optimized to determine the cited isomers in a binary mixture with a high accuracy. Under the optimized conditions, the results obtained showed that from cited methods used, the CC and RD methods have the least errors in determination of the concentration of highly spectral overlapping species. The mean percentage recovery values for the binary mixture of meta- and para-nitroaniline were found to be 95.98 and 98.78 using the RD method, and 101.85 and 100.23 using the CC method, respectively. For comparison of the powerful multivariate methods including the principal component regression (PCR) and partial least squares (PLS) were also applied. The mean percentage recovery values for the binary mixtures of the cited isomers were found to be 101.62 and 101.47 using the PCR method, and 101.00 and 101.36 using the PLS method, respectively. A statistical comparison of the methods demonstrated that there was no significant difference between the RD, CC, PCR, and PLS methods and the reported HPLC method regarding both accuracy and precision. The proposed methods have an admissible precision and accuracy for determination of the two isomers in their binary mixtures in tap water as a real sample.Insight into the mechanistic binding of bovine serum albumin (BSA) with doxofylline can layout pivotal enlightenment with relevance to pharmacokinetics and pharmacodynamics properties. Herein, many spectroscopic techniques and computational methods had been employed to interpret the structural and binding dynamics of BSA-doxofylline interaction. Doxofylline quenched the intrinsic fluorescence of BSA by static quenching. The stoichiometry and the binding constant of the BSA-doxofylline complex were 11 and in the order of 103 M-1. It was also concluded that the binding process was spontaneous and exothermic, primarily based on the thermodynamic study. Circular dichroism and three-dimensional excitation-emission matrix fluorescence results concluded pronounced conformational and microenvironmental changes in BSA structure on binding with doxofylline. The influence of metal ions and vitamins on the binding affinity of the BSA-doxofylline system were also explored. The in vitro findings were further supported by in silico analysis. With a score value of -6.25 kcal/mol, molecular docking showed strong interactions. Molecular dynamics simulation interpretation also suggested the stable binding with lower deviation in the values of RMSD and RMSF obtained by uninterrupted long simulation run. These studies will propose the optimum potency of distribution of the doxofylline into the bloodstream for asthma treatment.In this study, the binding of olanzapine (OLZ) to human serum albumin (HSA) and the influence of metal ions (Ca2+, Mg2+, Cu2+, Zn2+, Fe3+), caffeine (CAF) and flavonoids (diosmin (DIO), catechin (CAT), quercetin (QUE)), on their affinity, was investigated by fluorescence spectroscopy and UV-vis absorption spectroscopy. Fluorescence experiments suggest that OLZ quench the fluorescence of HSA through the mixed quenching mechanism and non-radiation energy transferring as a result of the HSA-OLZ complex formation. OLZ spontaneously bind in the site I on HSA, and according to thermodynamic parameters, the reaction was spontaneous and mainly driven by hydrogen bonds and van der Waals interactions. The presence of Mn+ ions, CAF, DIO and CAT decreased binding affinity between OLZ and HSA which indicates that they could compete against OLZ in the site I. WZ811 Contrary, in the presence of QUE the binding affinity of the HSA-OLZ system enhanced, which may be explained by conformational changes in HSA (non-competitive interference).
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