Notes

Homozygosity for any factor XII mutation in a woman then one male affected individual using genetic angio-oedema.
However, recent evidence has emerged to support the importance of the phytochelatin-independent pathway in heavy metal sequestration into the vacuole, with metal/H+ antiporters and proton pumps playing an important role. In this review, the importance of vacuolar proton pumps and metal/H+ antiporters transporting Cd, Cu, and Zn is discussed. In addition, the recent advances in the production of transgenic plants with potential application in phytoremediation and food safety through the manipulation of genes encoding V-PPase proton pumps is described.Rapeseed (Brassica napus) is an economically important oilseed crop in the world, but its production is strongly dependent on boron (B) supplies. Major intrinsic protein NIP5;1 is essential for B uptake and plant development under B limitation. In this study, phylogenetic and expression analyses identified two NIP5;1 orthologue genes, BnaA2.NIP5;1 and BnaA3.NIP5;1, which are mainly expressed in roots of B. napus. Specific and multiple-target RNAi was used to suppress BnaA3.NIP5;1 or both BnaA2.NIP5;1 and BnaA3.NIP5;1 expression in B-efficient rapeseed Qingyou 10 (QY10), respectively, for revealing the roles of BnaA2.NIP5;1 and BnaA3.NIP5;1 in low-B tolerance in B. napus. We found that both BnaA2.NIP5;1 and BnaA3.NIP5;1 are important for B. napus normal growth under low-B conditions, while these two genes have distinct roles. BnaA2.NIP5;1 is mainly expressed in the epidermis cells, which is required for efficient B uptake into roots, hence for B translocation to the shoots. BnaA3.NIP5;1 is specifically localized in the distal part of lateral root cap cells to promoter root elongation under low-B conditions, which is important for seed production in the maturity stage of B. napus. Taken together, our specific and multiple-target RNAi strategy provides novel insights into the gene function diversification between BnaA2.NIP5;1 and BnaA3.NIP5;1, such an approach can be potentially applicable to other polyploid crops.
Fungal contamination is a major cause of food spoilage. There is an urgent need to find and characterize natural preservatives. This study evaluates the prevalence of fungi in tomatoes and their control by using essential oil (EO) from sweet orange peel. Essential oils were extracted from dried and fresh sweet orange peels by using n-hexane and ethanol as extraction solvents. Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC-MS) analyses were performed to identify the chemical composition of the EO. A combination of chitosan (CS) and EO was used to control the fungal decay of tomatoes inoculated with Aspergillus niger and Penicillium citrinum.

Tomatoes obtained from local markets and supermarkets showed a high prevalence of Aspergillus and Penicillium spp. Essential oils extracted by ethanol from dried peels showed complete inhibition of A. niger and P. Dorsomorphin cell line citrinum and hyphal degradation at a minimum inhibitory concentration (MIC) of 100 μL mL
. The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C.

Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities. Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards. © 2021 Society of Chemical Industry.
Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities. Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards. © 2021 Society of Chemical Industry.
Streptococcus thermophilus, one of the most important lactic acid bacteria, is widely used in food fermentation, which is beneficial to improve food quality. However, the current genetic transformation systems are inefficient for S. thermophilus S-3, which hinders its further study.

We developed three electroporation transformation methods for S. thermophilus S-3, and optimized various parameters to enhance the transformation efficiency up to 1.3 × 10
 CFU/μg DNA, which was 32-fold higher than that of unoptimized. Additionally, transcriptional analysis showed that a series of competence genes in S. thermophilus S-3 were remarkedly up-regulated after optimization, indicating that improvement of transformation efficiency was attributed to the expression level of competence genes. Furthermore, to prove their potential, expression of competence genes (comEA, cbpD and comX) were employed to increase transformation efficiency. The maximum transformation efficiency was obtained by overexpression of comEA, which was 14-fold higher than that of control.

This is the first report of competence gene expression for enhancing transformability in S. thermophilus, which exerts a positive effect on the development of desirable characteristics strains. © 2021 Society of Chemical Industry.
This is the first report of competence gene expression for enhancing transformability in S. thermophilus, which exerts a positive effect on the development of desirable characteristics strains. © 2021 Society of Chemical Industry.Seed oils sourced from West Africa are generally not well-characterized, but likely to have an untapped potential. This review aims to make an overview of fatty acid (FA) composition of unconventional seed oils from semi-arid West African trees and evaluate potential for new and enhanced uses and for improving local livelihoods and biodiversity conservation. A total of 111 studies on FA composition were found, covering 31 species. Only 69 of the studies (62%) were included in the review, as 38% had unreliable or incomplete results. There was a clear link between taxonomic kinship and FA composition. Over 20 potentially interesting and underexploited oils were found, including oils with properties similar to palm oil, olive oil, coconut oil, shea butter, and cotton seed oil. About half of the oils have promising potential for cosmetics. One third of the oils were relatively saturated, indicating properties for structuring food and heat resistance. Most of the species had multiple uses and oil production could be profitable in co-production with other non-timber forest products.
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