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Avoid getting it as well as never spread that: researching self-interested as opposed to prosocial reasons for COVID-19 reduction behaviors.
Radioimmunoassay (RIA) methods have always represented a technique of choice for the determination of steroids in biological samples. The Amplified Luminescent Proximity Homogenous Assay-Linked Immunosorbent Assay (AlphaLISA) is now emerging as the new-generation immunoassay technology that does not require washing/separation steps. The aim of this study was to adapt the Perkin-Elmer's AlphaLISA kit for wool cortisol and compare it with a RIA wool cortisol assay. Wool from lambs, 35 at birth (A0) and 54 at two months old (A2), was collected and each extract was evaluated for wool cortisol concentrations (HCC) both by RIA and AlphaLISA immunoassay. The two methods showed good precision, sensitivity and specificity for determining HCC. Both methods were able to detect significant differences between the high and the low HCC assessed in lambs at A0 and A2 (P less then 0.01). learn more The HCC assessed with RIA were significantly higher than those assessed with AlphaLISA (P less then 0.01). Moreover, the correlation between HCC measured using the AlphaLISA and RIA methods was strong (r = 0.878). The regression analyses show a constant and not proportional error. This could be due to the diversity in the dosage steps and to the diversity of the molecules used in the two methods. Results support the validity of using AlphaLISA as an alternative method to RIA for the quantification of cortisol in sheep wool and considering the performances showed it has a great potential to be further applied as an excellent tool to evaluate HCC in samples derived from animal species.The purpose of this study was to determine the effectiveness of edible coatings of chitosan dissolved with liquid smoke in preserving meatballs. The liquid smoke was derived from rice hulls pyrolyzed at 340 °C. The edible coating was made by dissolving 0.5%, 1%, and 1.5% chitosan in 100 ml of liquid smoke at concentrations of 3% and 5%. Preservation was carried out by soaking the meatballs in the edible coating solution for 15 min and storing them at room temperature with observations every 6 h. Food resistance was examined using the Antibacterial Activity Test, Total Plate Count (TPC), and Total Volatile Base Nitrogen (TVB-N). The results of the antibacterial activity test showed that chitosan-dissolved liquid smoke had inhibition zones ranging from 6.49-7.07 mm against E. coli and 6.52-7.26 mm against Salmonella bacteria. The use of 5% concentrated liquid smoke reduced the number of bacterial colonies, with TPC values not below the SNI threshold after 48-hour storage. This indicates that liquid smoke has potential as an antibacterial. The TVB-N value doubled after 24 h, but the meatballs still had good freshness. After 54 h of storage time, the TVB-N value in all treatments exceeded the SNI threshold of 0.254 mgN/100 g, and the meatballs were no longer suitable for consumption.Previous reports demonstrated the utility of systemic application of growth hormone (GH) in the treatment of bone defects. Very few studies correlated bone repair efficacy with hepatic and renal side effects promoted by locally-delivered GH. The objectives of this study were to assess the bone repair properties along with hepatic and renal adverse effects promoted by local application of GH in a rat model. Thirty-two rats were randomly divided (4 groups; n = 8/group), as follows (i) AB (autogenous bone + local application of saline solution [SS]), (ii) AB+ (autogenous bone + SS local application + SS irrigation), (iii) AB/GH+ (autogenous bone + SS local application + GH irrigation) and (iv) AB/GHL+ (autogenous bone + GH local application + GH irrigation). Critical-sized defects (diameter = 5.0 mm) were surgically created by a single operator in the calvaria of rats. Defects were filled with ground autogenous bone. Defects pertaining to AB+ and AB/GH+ received a mixture of autogenous bone and a SS-saturated (0on were found in AB/GH+. Abnormal small-sized glomeruli were found in AB/GHL+. The utilization of autogenous bone graft associated with local application and irrigation with GH was shown to not improve the bone repair in calvarial critical-sized defects in a rat model.The present study aim to assess the effect of phosphate and urea fertilizers on the physicochemical properties, pH and electrical conductivity (EC) of the soil. The effect of these fertilizers on cation exchange capacity (CEC), organic matter (OM), and the possibility of contamination with heavy metals (HM) (Cr, Cu, Cd, Mn, Zn, Ni, Fe, and Pb) were studied on the soils of Alshati agricultural project at different seasons after forty years of fertilization. Uncultivated soil samples were also analyzed for comparison and considered as reference. Mean values of soil pH, EC, CEC, and OM ranged between 6.88-7.32, 0.14-0.26 μS/cm, 2.95-4.19 Cmol/kg and 0.49-0.53%, respectively, in all seasons. Concentrations of HMs were 9.50-38.38, 0.0-2.05, 0.0-0.47, 0.0-29.81, 0.0-13.85, 2.83-25.95 and 104-512.20 mg/kg respectively, for Cr, Cu, Cd, Mn, Zn, Ni and Fe. The concentrations of the HMs in the soil were found to be vary significantly with the seasons (winter, spring, summer, and autumn). However, no traces of Pb was found in the soil samples. The result showed a significant correlation between, pH, EC, CEC, OM and HM content of the soil. The geochemical index of contamination shows that there was no contamination with Cu, Cd, Zn, Pb, Cr, Mn, Ni, and Fe.Poor soil fertility and weed infestation are among major constraints to maize production in southern Africa. Nutrient and weed management strategies that are products of empirical research, are needed to improve efficiencies on farms. A field experiment was carried out in Eastern Zimbabwe on three smallholder farms positioned on upper, middle and lower catena. The farms differed in soil organic carbon (SOC) content, 3.9, 6.4 and 8.9 g kg-1 (hereafter referred to as low, medium and high), respectively, and are located within one km distance. The objective of the study was to investigate short-term (6 years) repeated application of soil nutrient amendments on maize productivity and weed dynamics across a soil fertility gradient. Treatments included strategic combinations of NPK fertiliser, cattle manure, and lime. On each farm, a randomised complete block design with three replicates was used. Multivariate, Principal Component Analysis, was used to establish the relationship between season, SOC content, nutrient management, and weed density.
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