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Endocarditis as a result of Trichosporon beigelii 14 decades soon after mitral valve substitute.
The operating characteristics of RoBoT are assessed through computer simulations and are compared with existing methods. Finally, we apply RoBoT to data from two recent phase II basket trials of imatinib and vemurafenib, respectively. © The Author 2020. Published by Oxford University Press. All rights reserved. For permissions, please e-mail [email protected] have identified an abscisic acid (ABA) sensitivity-associated gene from soybean, ABA-Sensitive 1 (GmABAS1), that belongs to the little-known 1R-subtype of MYB transcription factors. Here we describe its role as a transcriptional repressor which regulates ABA sensitivity. The truncated naturally mutated version, GmABAS1∆, was employed as a negative control. The nuclear localization of GmABAS1 was shown using the green fluorescent protein (GFP)-GmABAS1 fusion protein. The transcriptional repressor activity of GmABAS1 was demonstrated using yeast reporter systems. By overexpressing GmABAS1 in the transgenic soybean hairy root system, we identified the target gene of GmABAS1 to be Glyma.01G060300, an annotated ABI5 binding protein 3 (AFP3). Glyma.01G060300 and GmABAS1 exhibited reciprocal expression patterns under osmotic stress. The binding of GmABAS1 to the promoter of Glyma.01G060300 was demonstrated using electromobility shift assay (EMSA). The promoter binding and transcriptional repressor activities of GmABAS1 were further confirmed using a yeast promoter-binding reporter assay. The over-expression of GmABAS1 in Arabidopsis thaliana led to the down-regulation of AtAFP2 and enhanced sensitivity to ABA in the stomatal aperture and seed germination assays. This is the first time a 1R-subtype of MYB from soybean has been reported to be a transcriptional repressor involved in enhancing ABA sensitivity. © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.Virus-induced flowering (VIF) exploits RNA or DNA viruses to express flowering time genes to induce flowering in plants. Such plant virus-based tools have recently attracted widespread attention for their fundamental and applied uses in flowering physiology and in accelerating breeding in dicotyledonous crops and woody fruit-trees. We now extend this technology to a monocot grass and a cereal crop. Using the Foxtail mosaic virus-based VIF system, dubbed FoMViF, we showed that expression of florigenic Flowering Locus T (FT) genes can promote early flowering and spikelet development in proso millet, a C4 grass species with potential for nutritional food and biofuel resources, and in non-vermalized C3 wheat, a major food crop worldwide. Floral and spikelet/grain induction in the two monocot plants was caused by the virally expressed untagged or FLAG-tagged FT orthologues, and the florigenic activity of rice Hd3a was more pronounced than its dicotyledonous counterparts in proso millet. The FoMViF system is easy to perform and its efficacy to induce flowering and early spikelet/grain production is high. In addition to proso millet and wheat, we envisage that FoMViF will be also applicable to many economically important monocotyledonous food and biofuel crops. © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email [email protected] access trauma has important detrimental implications for immunological status, organ function and clinical recovery. Thoracic surgery has rapidly evolved through the decades, with the advantages of minimally invasive surgery becoming more and more apparent. The clinical benefits of enhanced recovery after video-assisted thoracoscopic surgery (VATS) may be, at least in part, the result of better-preserved cellular immunity and cytokine profile, attenuated stress hormone release and improved preservation of pulmonary and shoulder function. Parameters of postoperative pain, chest drain duration, hospital stay and even long-term survival are also indirect reflections of the advantages of reduced access trauma. With innovations of surgical instruments, optical devices and operative platform, uniportal VATS, robotic thoracic surgery and non-intubated anaesthesia represent the latest frontiers in minimizing trauma from surgical access. © The Author(s) 2020. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.OBJECTIVES We previously reported a transthoracic approach using the da Vinci Single-Site™ platform. Rapamycin inhibitor This study describes the outcomes of robotic single-site thymectomy via a subxiphoid approach based on our previous experiences. METHODS This study included patients, who underwent single-site robotic thymectomy via the subxiphoid approach between September 2018 and October 2019. A 3- to 4-cm vertical incision was made over the subxiphoid area, and the Lapsingle port with CO2 gas was introduced. After docking at the single site, a 5-mm curved cannula, 5-mm cardiere grasper and Maryland bipolar forceps or permanent cautery hook was inserted. The single-site robotic platform was used to conduct all surgical procedures except the dissection around the inferior portion of the thymic tissue performed via a thoracoscopic approach through the same incision. RESULTS Overall, 13 patients, including 2 with myasthenia gravis, were enrolled. The mean duration of the surgery and chest drainage were 167.3 ± 52.8 (range 73-253) min and 2.2 ± 0.9 (range 1-5) days, respectively. All surgeries were successfully completed without conversion to an open surgery, and there were no major post-surgical complications. The diagnoses based on histopathology included thymoma in 10 patients, thymolipoma in 2 patients and atypical carcinoid tumour in 1 patient with free resection margin. CONCLUSIONS This study demonstrated that robotic single-site-assisted thymectomy via the subxiphoid approach is a safe and technically feasible procedure. Although this initial series comprised relatively simple cases, more advanced and complex procedures can soon be performed with the advent of single-port robotic platform. © The Author(s) 2020. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.
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