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Wolbachia was more frequently found in females (32.73%), and only found in one male. Analysis of phylogenetic relationships, suggests that the two new Wolbachia sequences (wFran Frankliniella and wShan Scirtothrips hansoni) detected here represent two new groups for this endosymbiont. The haplotype network shows the presence of two possible haplotypes for each strain. Future studies to evaluate the possible use of Wolbachia as a control agent in avocado thrips are necessary.
The online version contains supplementary material available at 10.1007/s12088-021-00951-5.
The online version contains supplementary material available at 10.1007/s12088-021-00951-5.In this study, the medium requirements to increase the production of xylitol by using Candida tropicalis (CT) have been investigated. The technique of single addition or omission of medium components was applied to determine the nutritional requirements. The addition of amino acids such as Asp, Glu, Gln, Asn, Thr, and Gly had no significant effect on CT growth. However, in the absence of other metal ions, there was a higher concentration of cell growth and xylitol production when only Zn2+ was present in the medium. The analysis of various vitamins unveiled that biotin and thiamine were the only vitamins required for the growth of CT. Surprisingly, when only biotin was present in the medium as a vitamin, there was less growth of CT than when the medium was complete, but the amount of xylitol released was significantly higher. Overall, this study will increase the xylitol production using the single omission or addtion technique.
In this work, we synthesized blood orange peel extract-copper (II) (Cu
) ions nanoflower (NFs) and blood orange juice extract-copper (II) (Cu
) ions nanoflower examine their antimicrobial properties on the fish pathogen (
). The main compounds of the blood orange peel extract and the blood orange juice extract were organic components, and the copper (II) (Cu
) ions were inorganic components. BOPE-Cu
nanoflowers are quite compact, porous, and uniform as compared to BOJE-Cu
nanoflowers. Scanning Electron Microscopy, Fourier Transform Infrared spectrometry, and Energy-Dispersive X-ray spectroscopy were used to observe the structures of the NFs. The findings of FT-IR show Cu-O and Cu-N bonds in NF, which may be an indicator of the development of NFs. Although the antimicrobial actions of BOPE-hNFs and BOJE-hNFs against
(NCTC 12,268) have been confirmed.
Candidemia, one of the most common invasive fungal infections in hospitalized patients, can lead to death and huge financial losses. Candida albicans is the main causative agent of this disorder and Candida glabrata occupies the second or third place, for which new therapeutic alternatives must be found. The objective of the present study was to evaluate the inhibitory effect of etoposide and camptothecin (inhibitors of deoxyribonucleic acid (DNA) topoisomerase) on the C. MK-2206 molecular weight glabrata CBS138 strain. Etoposide and camptothecin showed better or similar MIC (minimum inhibitory concentration) (5 and 2.5 μg/mL, respectively), with respect to fluconazole (8 μg/mL) and itraconazole (4 μg/mL). They also suppressed colony formation during the 12-h test. On the other hand, petite colonies were less formed by exposing C. glabrata to etoposide or camptothecin (indicating low toxicity), with respect fluconazole and itraconazole. Such colonies are phenotypically observed as limited growth in medium containing a non-fermentable carbon source, and are genotypically characterized by a partial or total loss of mitochondrial DNA (mtDNA) fragments. Using PCR techniques and cell staining with 4',6-diamidino-2-phenylindole (DAPI), loss of mtDNA was detected only in yeast cells treated with fluconazole. Additionally, molecular docking studies with etoposide and camptothecin showed recognition in the active site of the Topo I and II enzymes from C. glabrata. Since etoposide and camptothecin showed good inhibitory activity and low toxicity on C. glabrata; they should certainly be of interest for the treatment of C. glabrata infections and the design and development of new antifungal compounds derived from these drugs.
The online version contains supplementary material available at 10.1007/s12088-021-00942-6.
The online version contains supplementary material available at 10.1007/s12088-021-00942-6.Gold nanoparticles with their excellent biocompatibility are extensively used in pharma and biological applications. Terminalia bellerica (TB) dry fruit parts mediated gold nanoparticles were synthesized using the aqueous extracts. The secondary metabolites screening of the aqueous extracts was done using phytochemical analysis. The green synthesized gold nanoparticles show vibrant colours. They were characterized using UV-Visible spectroscopy, FT-IR spectroscopy, XRD analysis and FE-SEM. The analytical characterization methods ensured the formation of nanoparticles and could predict the nanometric size of the nanoparticles. The study also lay to determine the antibacterial potential of the TB fruit parts and TB fruit parts mediated gold nanoparticles. The pathogens chosen for the study were pathogens from clinical species such as Acinetobacter pneumonia, Bacillus subtilis, and Enterococcus faecalis which cause common infections. The TB fruit part extracts, as well as TB fruit parts mediated gold nanoparticles were capable enough to destroy clinical pathogens.
The online version contains supplementary material available at 10.1007/s12088-021-00937-3.
The online version contains supplementary material available at 10.1007/s12088-021-00937-3.In this study, the aerial parts of Salvia hypargeia were subjected to hydrodistillation and the resulting compounds were analyzed in GC-MS. Antimicrobial activity of S. hypargeia essential oil (EO) against A. baumannii , S. aureus, and C. tropicalis were determined by agar well diffusion assay and microdilution method. Antimicrobial mechanism of the EO were investigated based on change of TTC-dehydrogenase relative, leakages of intracellular protein, DNA and potassium ion (K +). The main components of the EO were β-pinene, 1,8-cineole, camphor, α-pinene, 4-terpineol, and 4-thujanol. The MICs of the EO against the microorganisms were 15.2 mg/mL for S. aureus, 17.5 mg/mL for C. tropicalis and 28.8 mg/mL for A. baumannii. The inhibition zones were 18.16 mm, 25.01 mm, and 27.01 mm for A. baumannii, S. aureus, and C. tropicalis, respectively (p
The online version contains supplementary material available at 10.1007/s12088-021-00939-1.
The online version contains supplementary material available at 10.1007/s12088-021-00939-1.
Website: https://www.selleckchem.com/products/MK-2206.html
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