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The bulb decompose started in the neck and scatter to successive machines (Figure 1). In August 2015, onion cv. Redwing and Vaquero were seen having wet necrotic lesions building on leaves in the field (Figure 2). Margins of necrotic tissue, 1-2 cm3, were excised, surface sterilized, plated on liquid agar medium and incubated at 24°C. Hyphal development was sub-cultured from eight strains (A- D in 2014; E-H in 2015) to fresh potato dextrose agar to have pure countries. Countries were characteristic of Fusarium species as described by Nelson et al. (1983) utilizing the presence of microconidia formed on polyphialides with macroconidia present. Primers ITS4-A1 and ITS5 primers (White et al. 1990); EF-1 and EF-2 (O'Donnell et al. 1 storage in Idaho (Mohan et al. 1997). In inclusion, this is basically the first report for the fungus causing leaf disease in the field. These findings verify F. proliferatum because the causal broker of the high occurrence of bulb rot observed in 2014 and 2015. This bulb decompose will continue to occur in southwestern Idaho and since the pathogen causes leaf infections growers are encouraged to be aware both for leaf lesions through the growing period and bulb rot in storage.Vicia sativa subsp. nigra, also known as narrow-leaved vetch, is one of the household Fabaceae and it is distributed in different nations.Vetch is among the most crucial yearly forage legumes in the field; it is made use of as hay, grain, silage, green manure, and livestock fodder. In Korea, this plant is oftentimes used as cattle feed. During the early might of 2019 and 2020, a powdery mildew ended up being observed on leaves of V. sativa subsp. nigra flowers in a garden of Chonnam nationwide University, Gwangju, Korea. The first symptom appeared cathepsink in the top surface regarding the leaves as a little white area. Inside a fortnight, the white dots of powdery mildew spread and covered the entire leaf. Voucher specimens were deposited at the ecological Microbiology Laboratory Herbarium, Chonnam nationwide University, Gwangju, Korea, under the accession figures CNUFC PWV1 and CNUFC PWV2. The hyphal appressoria associated with fungi were nipple-shaped, reasonably lobed or multilobed, single or in opposite pairs. Dimensions were made of 35 conidiophores and 55 conormed by carefully pressing the contaminated leaves onto 10 youthful leaves of three healthy plants. A non-inoculated plant was utilized as a control. The flowers were preserved in a greenhouse at 22 to 25°C, with general humidity around 75%, and natural light. Powdery mildew symptoms were seen on the inoculated leaves after 10-14 times, whereas no symptoms were seen on the leaves associated with the control. The fungi in the inoculated leaves were morphologically just like the fungi on the original contaminated leaves. Erysiphe viciae-unijugae has been reported to occur on Lathyrus spp. and Vicia spp. (Farr and Rossman 2020). To the understanding, here is the first report of powdery mildew due to E. viciae-unijugae on V. sativa subsp. nigra in Korea. The weather and conditions, specially during May, are favorable for disease outbreaks. This illness is a possible threat to give manufacturing in Korea.In September of 2018, onion flowers (Allium cepa cv. Joaquin) cultivated within one field in southwest Idaho had been seen to have roots with brown stain over 10-20% associated with total root surface. Approximately 10% of plants over a 1 ha area had been affected and these flowers were about aesthetically 50% smaller compared to the standard bulb dimensions contained in the industry. To determine the causal broker, 3 mm bits of symptomatic origins from four plants were placed in salt hypochlorite (2%) for one min, followed by two rinses in sterile water and plated on to water agar method amended with penicillin G (0.2 g/liter) and streptomycin sulfate (0.8 g/liter). After 3 days at 21°C, fungal colonies with septate hyphae with right-angled branching resembling Rhizoctonia solani were seen in over 50 % of the 16 isolations tried. Species identification had been confirmed through rDNA the sequencing, as explained formerly (Woodhall et al., 2013), with DNA obtained from a single representative hyphal tip culture grown on Potato Dextrose Agarsuccessfully isolated from seven plant examples onto liquid agar. Sequencing had been used to ensure identification as AG2-2IIIB. To our knowledge, this is the very first report of R. solani AG 2-2 IIIB influencing onions in Idaho. Earlier operate in the Pacific Northwest recovered R. solani AG2-1, 3, 4 and 8 as well as BNR AG A from stunted onions (Patzek et al., 2013). In Japan, Misawa et al. (2017) discovered AG 2-2 IIIB is pathogenic to Welsh onion (Allium fistulosum). In Idaho, R. solani AG 2-2 IIIB has was previously reported causing illness in sugar beets (Strausbaugh et al. 2011) and potatoes (Woodhall et al. 2012). Growers must look into crop rotation techniques or earth treatments if R. solani AG2-2IIIB is causing disease in their crops.Erwinia amylovora is a plant-pathogenic bacterium that causes the fire blight disease in Rosaceae flowers. Since fire blight is extremely infectious and leads to serious losses once introduced, it is regulated as a quarantine disease. Recently, for the first time in East Asia, fire blight surfaced in Korea with strains of E. amylovora being isolated from lesions of infected woods. Five of these strains were selected and afflicted by whole-genome shotgun sequencing. Each strain had two circular replicons, a 3.8-Mb chromosome and a 28-kb plasmid. The genome sequences were in contrast to those of other E. amylovora strains isolated from various hosts or geographical regions. Genome synteny was analyzed and series variations including nucleotide substitutions, inversions, insertions, and deletions were detected. Analysis associated with population genomic framework unveiled that the five strains form a definite architectural team. Phylogenomic evaluation ended up being carried out to infer the evolutionary connections among E. amylovora strains, which suggested that the Korean isolates, all descended from a common ancestor, are closely linked to a lineage of North American strains. These outcomes provide helpful information for comprehending the genomic characteristics of E. amylovora strains including those who work in Korea, establishing hereditary markers for surveillance associated with the pathogen or analysis associated with disease, and finally establishing measures to eradicate it.
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