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They all face the challenge that knowledge about genebank accessions must be permanently managed and passed on across generations. International standards such as Multi-Crop Passport Descriptors (MCPD) have been established for the exchange of data between genebanks, and allow the operation of international information systems, such as the World Information and Early Warning System on Plant Genetic Resources for Food and Agriculture (WIEWS), the European Search Catalogue for Plant Genetic Resources (EURISCO) or Genesys.Clostridium perfringens is an important pathogen that is responsible for gastroenteritis; the causative agent for the symptoms is C. perfringens enterotoxin (CPE), which is mainly produced by type F C. perfringens. Since shellfishes may gather C. perfringens in the water environment, this study estimated the potential circulation of type F C. perfringens among humans, sewage, and Ruditapes philippinarum (asari clams) as a result of sewage pollution. A comparison of the characteristics among the isolates from 86 sewage influents, 36 effluents, 76 asari clams, and 37 humans was conducted. Serotyping, cpe genotyping, and toxin genotyping showed that C. perfringens with a plasmid IS1151 sequence downstream of cpe was predominant among sewage influents, effluents, humans, and asari clams. Multilocus sequence typing suggested that some isolates from a human, sewage influents, effluents, and asari clams were linked to each other. These results demonstrated that asari clams are the necessary infection sources of C. perfringens responsible for carriers and foodborne diseases, and that these pathogens from humans infected by asari clams can pollute the water environment. It is useful to assess bacteria such as C. perfringens isolates from sewage to estimate the trend of those from the community.New instruments to characterize vegetation must meet cost constraints while providing accurate information. In this paper, we study the potential of a laser speckle system as a low-cost solution for non-destructive phenotyping. The objective is to assess an original approach combining laser speckle with chemometrics to describe scattering and absorption properties of sunflower leaves, related to their chemical composition or internal structure. A laser diode system at two wavelengths 660 nm and 785 nm combined with polarization has been set up to differentiate four sunflower genotypes. REP-ASCA was used as a method to analyze parameters extracted from speckle patterns by reducing sources of measurement error. First findings have shown that measurement errors are mostly due to unwilling residual specular reflections. Moreover, results outlined that the genotype significantly impacts measurements. The variables involved in genotype dissociation are mainly related to scattering properties within the leaf. Moreover, an example of genotype classification using REP-ASCA outcomes is given and classify genotypes with an average error of about 20%. These encouraging results indicate that a laser speckle system is a promising tool to compare sunflower genotypes. Furthermore, an autonomous low-cost sensor based on this approach could be used directly in the field.In pediatric nephrotic syndrome, recommended mycophenolic acid (MPA) pharmacokinetics are higher than those for transplant recipients. In MPA therapeutic monitoring, inosine-5'-monophosphate dehydrogenase (IMPDH) activity may be useful. We modified the method established for renal transplant recipients and determined IMPDH activity in peripheral blood mononuclear cells (PBMCs) from healthy volunteers and children (4-16 years) with nephrotic syndrome treated with mycophenolate mofetil (MMF). From children, four blood samples were collected, and MPA concentrations were also determined. IMPDH activity was calculated using xanthosine monophosphate (XMP) normalized with adenosine monophosphate (AMP), both determined with the HPLC-UV method. The modified method was accurate, precise, and linear for AMP and XMP within 0.50-50.0 μmoL/L. Mean IMPDH activity in volunteers was 45.97 ± 6.24 µmoL·s-1·moL-1 AMP, whereas for children, the values were variable and amounted to 39.23 ± 27.40 µmoL·s-1·moL-1 AMP and 17.97 ± 15.24 µmoL·s-1·moL-1 AMP before the next MMF dose and 1 h afterward, respectively. The modified method may be applied to IMPDH activity determination in children with nephrotic syndrome treated with MMF. IMPDH activity should be determined after one thawing of PBMCs due to the change in AMP and XMP concentrations after subsequent thawing. Glucagon Receptor agonist For children, the lowest IMPDH activity was observed concomitantly with the highest MPA concentration.Vehicle detection is an indispensable part of environmental perception technology for smart cars. Aiming at the issues that conventional vehicle detection can be easily restricted by environmental conditions and cannot have accuracy and real-time performance, this article proposes a front vehicle detection algorithm for smart car based on improved SSD model. Single shot multibox detector (SSD) is one of the current mainstream object detection frameworks based on deep learning. This work first briefly introduces the SSD network model and analyzes and summarizes its problems and shortcomings in vehicle detection. Then, targeted improvements are performed to the SSD network model, including major advancements to the basic structure of the SSD model, the use of weighted mask in network training, and enhancement to the loss function. Finally, vehicle detection experiments are carried out on the basis of the KITTI vision benchmark suite and self-made vehicle dataset to observe the algorithm performance in different complicated environments and weather conditions. The test results based on the KITTI dataset show that the mAP value reaches 92.18%, and the average processing time per frame is 15 ms. Compared with the existing deep learning-based detection methods, the proposed algorithm can obtain accuracy and real-time performance simultaneously. Meanwhile, the algorithm has excellent robustness and environmental adaptability for complicated traffic environments and anti-jamming capabilities for bad weather conditions. These factors are of great significance to ensure the accurate and efficient operation of smart cars in real traffic scenarios and are beneficial to vastly reduce the incidence of traffic accidents and fully protect people's lives and property.
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