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Enzymatic task of navicular bone markers in Lithobates catesbeianus (Shaw, 1802) growth during the ossification procedure.
Samples of triticale seeds of various qualities were assessed in the study. The seeds were obtained during experiments, reflecting the actual sowing conditions. The experiments were conducted on an original test facility designed by the authors of this study. The speed of the air (15, 20, 25 m/s) transporting seeds in the pneumatic conduit was adjusted to sowing. The resulting graphic database enabled the distinction of six classes of seeds according to their quality and sowing speed. The database was prepared to build training, validation and test sets. The neural model generation process was based on multi-layer perceptron networks (MLPN) and statistical (machine training). When the MLPN was used to identify contaminants in seeds sown at a speed of 15 m/s, the lowest RMS error of 0.052 was noted, whereas the classification correctness coefficient amounted to 0.99.The purpose of this study was to analyze the body balance control of people walking and hurdling with or without a specific dominant leg in a monopodal position. This study involved 28 physical education students. The center of pressure (COP) was measured with a force plate under four conditions single-leg standing (right and left) with eyes open and two upper limb positions (the arms were positioned in either a specific hurdle technique manner or alongside the body). A repeated measures analysis of variance (ANOVA) was conducted separately for five parameters of the COP in the medial-lateral (ML) and anterior-posterior (AP) directions under the four conditions. In the single-leg position, athletes without a dominant lower leg had better body balance than those with a dominant lower leg. The position of the upper limbs influenced the body position when hurdling. Accepting the correct position of the upper limbs helped to maintain balance (when overcoming hurdles). In hurdles, the position of the upper limbs should be improved to maintain postural stability and save this function for more demanding postural tasks.The stromal vascular fraction (SVF) consists of a heterogeneous population of stem and stromal cells, generally obtained from adipose tissue by enzymatic digestion. Erastin For human cell-based therapies, mechanical process methods to obtain SVF represent an advantageous approach because they have fewer regulatory restrictions for their clinical use. The aim of this study was to characterize a novel commercial system for obtaining SVF from adipose tissue by a mechanical approach without substantial manipulations. Lipoaspirate samples collected from 27 informed patients were processed by a simple and fast mechanical system (by means of Hy-Tissue SVF). The Hy-Tissue SVF product contained a free cell fraction and micro-fragments of stromal connective tissue. The enzymatic digestion of the micro-fragments increased the yield of free cells (3.2 times) and CFU-F (2.4 times). Additionally, 10% of free cells from SVF were positive for CD34+, suggesting the presence of endothelial cells, pericytes, and potential adipose-derived stem cells (ADSC). Moreover, the SVF cells were able to proliferate and differentiate in vitro toward adipocytes, osteocytes, and chondrocytes. The immunophenotypic analysis of expanded cells showed positivity for typical mesenchymal stem cell markers. The Hy-Tissue SVF system allows the isolation of stromal vascular fraction, making this product of potential interest in regenerative medicine.This paper presents an ultrasound transceiver application-specific integrated circuit (ASIC) directly integrated with an array of 12 × 80 piezoelectric transducer elements to enable next-generation ultrasound probes for 3D carotid artery imaging. The ASIC, implemented in a 0.18 µm high-voltage Bipolar-CMOS-DMOS (HV BCD) process, adopted a programmable switch matrix that allowed selected transducer elements in each row to be connected to a transmit and receive channel of an imaging system. This made the probe operate like an electronically translatable linear array, allowing large-aperture matrix arrays to be interfaced with a manageable number of system channels. This paper presents a second-generation ASIC that employed an improved switch design to minimize clock feedthrough and charge-injection effects of high-voltage metal-oxide-semiconductor field-effect transistors (HV MOSFETs), which in the first-generation ASIC caused parasitic transmissions and associated imaging artifacts. The proposed switch controller, implemented with cascaded non-overlapping clock generators, generated control signals with improved timing to mitigate the effects of these non-idealities. Both simulation results and electrical measurements showed a 20 dB reduction of the switching artifacts. In addition, an acoustic pulse-echo measurement successfully demonstrated a 20 dB reduction of imaging artifacts.Populus alba 'Villafranca' clone is well-known for its tolerance to cadmium (Cd). To determine the mechanisms of Cd tolerance of this species, wild-type (wt) plants were compared with transgenic plants over-expressing an aquaporin (aqua1, GenBank GQ918138). Plants were maintained in hydroponic conditions with Hoagland's solution and treated with 10 µM of Cd, renewed every 5 d. The transcription levels of heavy metal transporter genes (PaHMA2, PaNRAMP1.3, PaNRAMP2, PaNRAMP3.1, PaNRAMP3.2, PaABCC9, and PaABCC13) were analyzed at 1, 7, and 60 d of treatment. Cd application did not induce visible toxicity symptoms in wt and aqua1 plants even after 2 months of treatment confirming the high tolerance of this poplar species to Cd. Most of the analyzed genes showed in wt plants a quick response in transcription at 1 d of treatment and an adaptation at 60 d. On the contrary, a lower transcriptional response was observed in aqua1 plants in concomitance with a higher Cd concentration in medial leaves. Moreover, PaHMA2 showed at 1 d an opposite trend within organs since it was up-regulated in root and stem of wt plants and in leaves of aqua1 plants. In summary, aqua1 overexpression in poplar improved Cd translocation suggesting a lower Cd sensitivity of aqua1 plants. This different response might be due to a different transcription of PaNRAMP3 genes that were more transcribed in wt line because of the importance of this gene in Cd compartmentalization.
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