Notes

Part regarding intraluminal brachytherapy like a palliative therapy modality within unresectable cholangiocarcinomas.
The giant panda (GP) is the most precious animal in China. Gastrointestinal tract disease, especially associated with dysbiosis of gut microbiota, is the leading cause of death in GPs. Here, we performed 16S rRNA high-throughput sequencing to investigate the gut microbiota of GPs having symptoms of anorexia. Results showed that gut microbiota of GP with anorexia had lower richness (Chao1 index) than the healthy GP. However, no significant differences in alpha diversity were observed. There is a significance in the microbial structure between anorexia and healthy GPs. The abundance of phylum Firmicutes (99.23% ± 7.1%), unidentified genus Clostridiales (24.75% ± 2.5%), was significantly higher in the subadult anorexia group (P  0.6, P  less then  0.05). Our results suggested that Streptococcus might be used as probiotics to control the growth of Clostridium causing the anorexia.Wolbachia is a maternally inherited bacterium of insects that can alter the reproduction, biology, and fitness of the hosts. It was detected in natural populations of the Yamatotettix flavovittatus Matsumura leafhoppers, the vector of phytoplasma, which is responsible for sugarcane white leaf disease. Wolbachia infection prolongs the longevity of female leafhoppers and promotes a strong reproductive incompatibility; importantly, highly maternal transmission rate was observed. However, limited data on the diversity or strain typing of Wolbachia in Y. flavovittatus are available. We aimed here to detect the presence of Wolbachia in different populations by amplification of the wsp gene, which was then sequenced. Multilocus sequence typing (MLST) was also performed to explore the diversity of the Wolbachia strains. Based on the wsp sequences, Wolbachia in the Y. flavovittatus leafhoppers belonged to supergroup B, and formed a distinct evolutionary lineage; therefore, we designated this new specific strain as wYfla. The MLST profiles revealed ten potential new sequence types (STs) in different leafhopper populations. Multiple STs were detected in individual leafhoppers, among which the ST-wYfla1 strain was predominant. Furthermore, we obtained congruent results for the phylogenetic analyses using the wsp gene and MLST loci. To the best of our knowledge, this is the first study characterizing Wolbachia strains in Y. selleck kinase inhibitor flavovittatus. Our results reveal a novel strain and multiple STs of Wolbachia, and these data may prove useful in the exploitation of Wolbachia as a biological Y. flavovittatus control agent.Use of heavy metal (HM) resistant plant growth-promoting rhizobacteria (PGPR) is among the eco-friendly strategies to increase the resistance of crop plants against the HM stress. In this study, we investigated the effects of two copper (Cu)-resistant PGPR strains (Pseudomonas fluorescens P22 and Pseudomonas sp. Z6) on the growth and nutrition of maize (Zea mays) and sunflower (Helianthus annuus) plants grown in a Cu-contaminated soil under glasshouse conditions. Both PGPR strains significantly increased the plant vegetative parameters including shoot biomass, stem height and diameter, and chlorophyll (SPAD values) index in both crops. In both plants, the PGPR inoculations also significantly elevated the uptake of nutrients including potassium, phosphorus, calcium, magnesium (only by P. fluorescens P22), iron, zinc, manganese, and Cu. Magnitude of the nutritional effects varied between the PGPR strains, e.g., in sunflower, inoculation with P. fluorescens P22 and Pseudomonas sp. Z6 led to an increase in uptake of Zn by 42% and 114%, or Mn by 61% and 88%, respectively, in comparison with control plants. Improved performance of the inoculated plants can be attributed to the plant growth-promoting (e.g., production of auxin and siderophore, phosphate solubilization activities, etc.) and stress removal (e.g., production of ACC-deaminase to drop the ethylene level in stressed plants) properties of the PGPR strains, which were uncovered in our in vitro studies prior to the glasshouse experiment. Beside the plant growth-promoting traits of these PGPR strains, their high resistance to Cu toxicity seemed to be of particular importance for plant fitness improvement under Cu toxicity.The widespread use of harmful fungicides in the agricultural sector has led to a demand for safer alternatives to protect against crop pathogens. The domestic apple is the second most highly consumed fruit in the world and encounters several pre- and post-harvest fungal and bacterial phytopathogens. The goal of this study was to explore the uncharacterized microbiome of a wild apple, Malus trilobata, as a potential source of novel biocontrol agents for two post-harvest fungi that affect commercial apples Botrytis cinerea and Penicillium expansum. We sampled microflora associated with the leaves, bulk soil, and roots of Malus trilobata in two regions of Lebanon Ehden reserve in the north and Dhour EL Choueir near Beirut. The two regions have different soil types Dhour EL Choueir and samples from the two regions showed very different microbial compositions, with greater microbial diversity among those from Ehden reserve. Molecular characterization revealed a wide variety of genera displaying activity against the two fungal pathogens, including several with previously unknown antifungal activity Bosea, Microlunatus, Microbacterium, Mycetecola, Rhizobium and Paraphoma. In total, 92 strains inhibited Penicillium expansum (39%) and 87 strains inhibited Botrytis cinerea (38%) out of 237 screened. Further chemical and genetic characterization of one or more selected strains could pave the way for future development of new biocontrol agents for post-harvest applications.
To identify factors in community pharmacy that facilitate error recovery from medication incidents (MIs) and explore medication safety prevention strategies from the pharmacist perspective.

Thirty community pharmacies in Sydney, Australia, participated in a 30-month prospective incident reporting program of MIs classified in the Advanced Incident Management System (AIMS) and the analysis triangulated with case studies. The main outcome measures were the relative frequencies and patterns in MI detection, minimisation, restorative actions and prevention recommendations of community pharmacists.

Participants reported 1013 incidents with 831 recovered near misses and 165 purported patient harm. MIs were mainly initiated at the prescribing (68.2%) and dispensing (22.6%) stages, and most were resolved at the pharmacy (76.9%). Detection was efficient within the first 24 h in 54.6% of MIs, but 26.1% required one month or longer; 37.2% occurred after the patient consumed the medicine. The combination of specific actions/attributes (85.
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