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Angiotensin-converting molecule Only two reduces lung artery high blood pressure levels by way of inhibition involving major adhesion kinase phrase.
05）, and group 7 was the largest. There was significant difference among group 4, 5, 6 in average energy absorption ratio（P less then 0.05）, among which group 4 and group 5 were larger. CONCLUSIONS 3 mm splint is good enough to be used to make mouthguard, which is also thinner and more comfortable. Derazantinib concentration Splint of soft material is more suitable for mouthguard than splint of hard material.PURPOSE To investigate the effect of low-magnitude high frequency vibration (LMHFV) on proliferation, migration ability and osteogenic differentiation of human periodontal ligament stem cells(hPDLSCs). METHODS hPDLSCs were isolated from premolar and randomized into vibration culture group (magnitude：0.3 g; frequency：40 Hz; time：15 min/24 h) and static culture group. CCK-8 was used to identity the proliferation of hPDLSCs. Wound-healing assay was used to evaluate migration ability of hPDLSCs. The osteogenesis gene expression was analyzed by RT-PCR, and the osteogenesis protein expression was analyzed by Western blot. The osteogenesis differentiation capability was evaluated by alizarin red staining. The data were analyzed using SPSS 21.0 software package. RESULTS After LMHFV, the proliferation and migration ability of hPDLSCs were increased. The expression level of RUNX2, ALP, Col-1, and OCN was significantly augmented under LMHFV. Alizarin red staining and Western blot proved the same trend. CONCLUSIONS：The results demonstrate that LMHFV can promote hPDLSCs proliferation, migration ability and osteogenic differentiation.PURPOSE To explore the combined effect of recombinant human transforming growth factor-β1 (rhTGF-β1) and recombinant human platelet derived growth factor-BB (rhPDGF-BB) on the expression of Pyk2 in the osteoclasts of the pressure side of orthodontic tooth in rats. METHODS One hundred and sixty male Sprague-Dawley rats were randomly divided into 2 groups experimental group(A) and control group(B) to establish orthodontic tooth movement model. Rats in the experimental group received combined injection of 5 ng rhTGF-β1 and 10 ng rhPDGF-BB in the buccal submucosal area of the left upper first molar every other day, while rats in the control group received equivalent volumes of PBS. Rats in each group were sacrificed at each of 5 time points(1,4,7,10 and 14 days) after appliance placement. The distance of the tooth movement was measured with stereomicroscope, the change of the amount of osteoclasts was detected with tartrate-resistant acid phosphatase histochemistry (TRAP), the protein and mRNA expression of Pyk2 was detected by immunohistochemistry and real time PCR(RT-PCR). SPSS19.0 software package was used for statistical analysis. RESULTS The distance of teeth in group A moved more rapidly than in group B, except for the first day, significant differences were found (P less then 0.05) in all time points.The number of osteoclasts in group A was significantly higher than that in group B, except for the 14th day, compared with the control group(P less then 0.05). Pyk2 protein and gene expression of group A was significantly higher than group B, both groups reached a peak in 7 days and gradually decreased thereafter, except in the first day, the expression of protein Pyk2 was significantly different at other time points (P less then 0.05). CONCLUSIONS rhTGF-β1 and rhPDGF-BB up-regulated the expression of Pyk2 protein and mRNA gene on the pressure side of orthodontic tooth in rats, which may be one of the reasons for accelerating the rate of orthodontic tooth movement.PURPOSE To evaluate the mechanical properties and cytotoxicity of a 3D printing polycarbonate material used in digital occlusal splints, and to use this material to manufacture a splint with CAD/CAM technology. METHODS Specimen of two different materials, 3D printing polycarbonate(PC-plus) and transparent base resin (PMMA) were processed. The flexural strength, elastic modulus, microhardness, hygroscopicity and water-solubility of these materials then were evaluated. According to the standard of GBT16886.5-2003, cell culture and cytotoxicity test in vitro were conducted to evaluate the target materials on the morphology, growth and proliferation of cultured cells (L929). By using 3D printing technology, a splint was made of this 3D printing polycarbonate material. Statistical analysis was performed using SPSS 24.0 software package. RESULTS For the group of PC-plus, the flexural strength ranged from 89.4 to 109.8 MPa, the elastic modulus from 1939.4 to 2470.9 GPa, the microhardness from 15.6 to 24.7 MPa, hygroscopicity from 2.43 to 11.42 μg/mm3 and water-solubility from 0.11 to 0.30 μg/mm3. For PMMA group, the flexural strength ranged from 75.2 to 88.4 MPa, the elastic modulus from 1349.2 to 2470.2 GPa, the microhardness from 17.5 to 35.3 MPa, hygroscopicity from 12.80 to 16.16 μg/mm3 and water-solubility from 4.74 to 7.44 μg/mm3. The difference between 3D printing PC-plus group and PMMA base resin group was statistically significant (P less then 0.05). L929 cells showed normal morphology and proliferation increased with culture time. The toxicity grade of all groups was 0-1, and the 3D printing splint was made successfully. CONCLUSIONS The polycarbonate material for 3D printing has adequate mechanical properties and biocompatibility to meet the requiement of clinical application.PURPOSE The effects of different compounds on dry socket were evaluated in order to find a new method that can both be antibacterial and osteogenic,providing experimental evidence for future clinical application. METHODS Seventy-two male SD rats, with upper left anterior teeth been extracted, were infected by pus to result in dry socket.Seven days later, they were allocated randomly and evenly into 4 groups and received different treatment, i.e. group A debridement; group B debridement and filled with iodoform gauzes; group C debridement and filled with periocline; group D debridement and filled with TC-PHBHHx/β-TCP. After being treated for 1,4,8 weeks, sequential fluorescent labeling was performed. The animals were sacrificed after the procedure and hard tissue and decalcified sections were harvested for histological and histomorphometrical evaluation. Statistical analysis was performed using SPSS 19.0 software package. RESULTS At the same time point, the results of osteogenesis in group A, B and C were not significantly different while the results in group D was significantly different from other groups; accordingly, significant new bone formation was observed.
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