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Di-n-butyl phthalate (DBP), one of the most widely used plasticizers, has been listed as a priority pollutant because of its toxicity to both humans and animals. In this study, Pseudomonas sp. W1, isolated from activated sludge, was capable of degrading 99.88% of DBP (1000 mg L-1) within 8 days. We immobilized the W1 strain using Fe3O4 iron nanoparticles (IONPs) coated with poly-dopamine (PDA), and further evaluated its DBP degradation efficiency. The DBP degradation performance of W1 was improved by immobilization, exhibiting 99.69% of DBP degradation efficiency on the 6th day, which was 25.68% higher than un-immobilized W1. After three cycles of magnetic recycling and utilization, W1-PDA-IONPs retained 99.6% of their original efficiency. W1-PDA-IONPs were then used to degrade DBP in landfill leachate. When the proportion of raw leachate was ≤50%, DBP could be all degraded by W1-PDA-IONPs within 6 days. In 100% landfill leachate, DBP degradation efficiency after 10 days of incubation reached 66.40%. Furthermore, W1-PDA-IONPs cells in a simulated aeration system could be effectively magnetically separated at aeration rates from 60 to 600 mL min-1. These results highlight the potential of W1-PDA-IONPs in the bioremediation of DBP-contaminated waste water. In the present work, in-situ two pot trials were conducted to explore the direct and residual influences of zeolite (ZL) on plant height, dry biomass and bioavailability of Pb, Cd, Cu, and Zn by growing cabbage followed by corn in goldmine-contaminated (GM-C), smelter factory-contaminated (SF-C), and farmland-contaminated (FL-C) soils. Initially, a single treatment of ZL was applied at 20 t/ha, and cabbage was grown under greenhouse pot conditions. After cabbage harvesting, corn was grown in the same pots without additional application of ZL. The results indicated that ZL as an amendment evidently promoted the cabbage and corn yields, whereas the residual influence of ZL did not promote corn dry matter yield in SF-C and FL-C soils compared to CK. Incorporation of ZL potentially decreased the mobility of Pb, Cd, Cu and Zn in contaminated soils after harvesting cabbage and corn compared with CK. In both crops, the Pb, Cd, Cu and Zn contents in plants root and shoot biomasses were dramatically reduced by the direct and residual impacts of ZL rather than CK. PT2385 purchase This study highlights that the direct and residual influences of ZL at a 20 t/ha application rate have the possibility to support the reclamation of soils polluted with harmful elements and that, by itself, ZL can promote plant growth and increase the value of field crops. The detailed studied regarding residual influence of ZL for restoration of multi-metal polluted soils would be confirmed at the ex-situ condition. Per- and polyfluoroalkyl substances (PFASs) are artificial refractory organic pollutants which are widely presented in aqueous environment. Due to the unquiet strength of the highly polarized carbon-fluorine bond (C-F) and their hydrophobic/lipophobic feature as well as biological persistence properties, the remediation and treatment of PFASs is a big challenge. Preliminary studies indicate that a few kinds of technical approaches could remove or transfer PFASs, but the effectiveness is not high as expected or limited while most of the techniques are only tested at laboratory scale. A review of existing treatment technologies was thus conducted for the purpose to outlook these technologies, and more importantly, to propose the foreseeable technique. As such, a constructed wetland-microbial fuel cell (CW-MFC) technology was recommended, which is a newly emerged technology by integrating physical, chemical and enhanced biological processes plus the wetland plants function with strong eco-friendly feature for a comprehensive removal of PFASs. It is expected that the review can strengthen our understanding on PFASs' research and thus can help selecting reasonable technical means of aqueous PFASs control. The main aim of the study was to compare the sorption capacity of hay-based activated biochars, obtained using conventional and microwave furnance, relative to copper(II) ions and ionic polyacrylamides (PAM). Surface properties of the solids were characterized by, inter alia, N2 adsorption/desorption isotherm method, whereas their tendency to aggregation was established turbidimetrically. Adsorption capacity of biochars were performed in the simple and mixed Cu(II)/PAM systems, i.e. the examined suspensions contained one or two adsorbates at the same time. The results indicated that biochar prepared in microwave furnance was characterized by larger micropore area and, as a result, it had higher adsorption capacity relative to Cu(II) ions. At pH 6, when the initial Cu(II) concentration equaled 100 mg/L, the biochar obtained by microwave heating adsorbed 81.5% of Cu(II) ions, whereas the one obtained by conventional heating - 51.6%. Due to high molecular weight, the PAM macromolecules could not penetrate the biochar micropores and thus the polymer adsorbed amounts were similar for both materials. For initial polymer concentration equal to 100 mg/L, the solids adsorbed 65-66.2% of cationic PAM containing 25% of quaternary amine groups. In the mixed system of anionic polyacrylamide and Cu(II) ions, the formation of Cu(II)-PAM complexes occurred, which favored both heavy metal and polymer adsorption on the solid surface. On the other hand, cationic polyacrylamide and heavy metal ions made the contact with the solid difficult for each other. What is more, ionic polyacrylamide and copper(II) ions stimulated the biochar aggregation due to surface charge neutralization and flocculation. This study demonstrates the feasibility of using Exiguobacterium sp. AO-11 to remediate oil-contaminated environments. Bioaugmentation using AO-11 showed the best removal percentage, 75%, of 4% (w/w) crude oil in sediment microcosms in 100 days. In terms of the bacterial community structure during crude oil degradation, the addition of AO-11 did not change the indigenous bacterial community, while the addition of urea fertilizer induced structural shift of indigenous bacterial community. Exiguobacterium sp. AO-11 was developed as a bioremediation product, and a liquid formulation of AO-11 was developed. Coconut milk residue and soybean oil mill sludge were used for bacterial cultivation to reduce the production cost, and they could enhance bacterial cell growth. The liquid formulation of AO-11 prepared in phosphate buffer could be stored at 4 °C for at least 2 months, and it maintained efficacy in the treatment of crude oil-contaminated seawater. Overall, bioaugmentation with strain AO-11 could be an effective solution for the bioremediation of crude oil-contaminated environments.
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