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The plasma membrane of eukaryotic cells is known to be compositionally asymmetric. Certain phospholipids, such as sphingomyelin and phosphatidylcholine species, are predominantly localized in the outer leaflet, while phosphatidylethanolamine and phosphatidylserine species primarily reside in the inner leaflet. While phospholipid asymmetry between the membrane leaflets is well established, there is no consensus about cholesterol distribution between the two leaflets. We have performed a systematic study, via molecular simulations, of how the spatial distribution of cholesterol molecules in different "asymmetric" lipid bilayers are affected by the lipids' backbone, head-type, unsaturation, and chain-length by considering an asymmetric bilayer mimicking the plasma membrane lipids of red blood cells, as well as seventeen other asymmetric bilayers comprising of different lipid types. Our results reveal that the distribution of cholesterol in the leaflets is solely a function of the extent of ordering of the lipids within the leaflets. The ratio of the amount of cholesterol matches the ratio of lipid order in the two leaflets, thus providing a quantitative relationship between the two. These results are understood by the observation that asymmetric bilayers with equimolar amount of lipids in the two leaflets develop tensile and compressive stresses due to differences in the extent of lipid order. These stresses are alleviated by the transfer of cholesterol from the leaflet in compressive stress to the one in tensile stress. These findings are important in understanding the biology of the cell membrane, especially with regard to the composition of the membrane leaflets.Acute organophosphorus pesticide poisoning (AOPP) is a worldwide health concern that has threatened human lives for decades, which attacks acetylcholinesterase (AChE) and causes nervous system disorders. Classical treatment options are associated with short in vivo half-life and side effects. As a potential alternative, delivery of mammalian-derived butyrylcholinesterase (BChE) offers a cost-effective way to block organophosphorus attack on acetylcholinesterase, a key enzyme in the neurotransmitter cycle. Yet the use of exotic BChE as a prophylactic or therapeutic agent is compromised by short plasma residence, immune response and unfavorable biodistribution. To overcome these obstacles, BChE nanodepots (nBChE) composed of a BChE core/polymorpholine shell structure were prepared via in situ polymerization, which showed enhanced stability, prolonged plasma circulation, attenuated antigenicity and reduced accumulation in non-targeted tissues. In vivo administration of nBChE pre- or post-organophosphorus exposure in a BALB/C mouse model resulted in potent prophylactic and therapeutic efficiency. To our knowledge, this is the first systematic delivery of non-human BChE to tackle AOPP. In addition, this work also opens up a new avenue for real applications in both research and clinical settings to cope with acute intoxication-related diseases.Fractions of dyes, that are used in large quantities for various applications, are lost during the dying process and contaminate water. In order to avoid their harmful effect on human health, boron nitride nanosheets (BNNSs) have been synthesized in this work and their adsorption behavior for the removal of anionic methyl orange (MO) dye from aqueous solution has been reported. The effect of pH, contact time, and initial dye concentration has been investigated on MO to find the optimum pH, equilibrium and adsorption capacity of the synthesized BNNSs. The adsorption kinetics and isotherm models showed that pseudo-second-order (PSO) kinetic and Freundlich isotherm models were being followed during the adsorption, respectively. The experimental maximum adsorption capacity of the synthesized adsorbent was found to be 575.0 mg g-1, which is due to the strong electrostatic attraction between the negatively charged MO and positively charged BNNSs. Furthermore, density functional theory (DFT) calculations have also been performed to investigate the nature and feasibility of the adsorption process, the interactions of MO dye molecules with the adsorbent, and the adsorption capacity of BNNSs. The theoretical and experimental studies suggest that the adsorption process is physical in nature. It was found that negative charge transfer occurred from MO to BNNSs with high chemical potential suggesting high chemical activity and a decrease in band gap after the adsorption process. These theoretical and experimental findings demonstrate the potential of BNNSs as adsorbents for commercial applications.Although small cell lung cancer (SCLC) is characterized by early metastasis and high resistance to most anti-cancer therapeutics, resulting in poor prognosis, surgical treatment is unavailable for most patients. Instead, clinical treatment for SCLC patients relies largely on chemotherapy. Therefore, an analysis platform supporting research into the physiology of SCLC cells and novel anti-cancer drugs is strongly needed. Decellularized extracellular matrix (dECM) hydrogel is a promising candidate cell-culture system that could provide a tissue-specific environment. However, dECM-based hydrogels have limited property control, poor mechanical properties, and loss of components during decellularization. click here In this study, porcine decellularized lung tissue and hyaluronic acid (HA) were hybridized via photopolymerization to form a pulmonary tissue-mimetic hydrogel. dECM solution was obtained by decellularization and pepsin digestion. The dECM and HA were then modified with methacrylic moieties, which produced dECM-methacrylate (dECM-MA) and HA methacrylate (HA-MA). dECM-MA/HA-MA hydrogels were fabricated by photopolymerization using a photoinitiator under UV light irradiation. The mechanical properties of the dECM-based hydrogel were compared with those of native tissue. SCLC cells (NCI-H69) were encapsulated in multiple types of dECM-based hydrogels, and they exhibited higher cell proliferation, drug resistance, and CD44 expression in the presence of dECM-MA and HA-MA than in the control condition.
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