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Pediatric Dealing During Venipuncture With Digital Reality: Preliminary Randomized Governed Trial.
miR-15a may serve as a promising biomarker for early diagnosis of CAD.
The present study elucidated a decreased miR-15a expression in CAD patients and showed negative correlations with LDL-C, Gensini score, and inflammatory cytokines. miR-15a may serve as a promising biomarker for early diagnosis of CAD.
Cell population data (CPD) are leukocyte morphologic parameters, measured by automated hematology analyzers with VCS technology. Many studies have demonstrated that these parameters may have clinical utility for diagnosing or screening certain pathological conditions. This study is to investigate the effects of peripheral blood stored at room temperature on the CPD values and provide useful information about storage-induced CPD changes.

Venous blood samples from healthy donors kept at room temperature (18 - 25°C) for different time intervals were analyzed using a Coulter DxH800 hematology analyzer. The CPD data collected included mean cellular volume, conductivity and multiple angles of light scatters as well as their corresponding standard deviation for neutrophils, lymphocytes, and monocytes. Peripheral blood smears at each time interval were also prepared and examined microscopically.

Peripheral blood kept at room temperature over time significantly affects the CPD values for neutrophils, lymphocytes, and monocytes. These CPD changes are correlated with the morphologic alterations observed under light microcopy, but detected much earlier. Some changes imitate clinical pathological conditions. For example, aged neutrophils showed decreased median angle light scatters, suggesting cytoplasmic degranulation, which can be seen in the case of myelodysplastic syndrome.

This study provides valuable information about storage-induced CPD changes that can affect potential clinical application and interpretation of these automated digital morphologic parameters.
This study provides valuable information about storage-induced CPD changes that can affect potential clinical application and interpretation of these automated digital morphologic parameters.
Factor XIII deficiency is one of the most severe congenital bleeding disorders with high rate of life-threatening bleeding including central nervous system bleeding, umbilical cord bleeding, and recurrent miscarriages. Due to the highest global incidence of the disorder in Iran, this study aimed to design a premarital screening program in Iran.

This descriptive study was performed on 30 couples with a positive family history of factor XIII deficiency. Underling F13A gene mutation was determined in the family members, and all the selected couples underwent molecular testing mutations by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), tetra primer-amplification refractory mutation system-PCR (T-ARMS-PCR), and sequencing.

The probability of affected childbirth existed for ten couples. Three couples decided not to marry, while seven got married and three of them decided to have a baby. MS023 The genotypes of the fetuses were determined and revealed that none of them was a homozygote for the F13A gene mutation.

Because of the importance of factor XIII deficiency diagnosis, it can be helpful to control the incidence of factor XIII deficiency by implementing preventive programs such as premarital screening.
Because of the importance of factor XIII deficiency diagnosis, it can be helpful to control the incidence of factor XIII deficiency by implementing preventive programs such as premarital screening.
Diagnostics utility of the obtained laboratory test results strictly depends on their possible interpretation in the context of distinguishing of both the health and disease state as well as the desired effects of therapeutic interventions. For this purpose, it is necessary to compare the obtained results to the reference intervals. Current recommendations obligate each laboratory to determine its own reference intervals for each of examined parameters. Many laboratories are unable to define and implement their own reference intervals because it is an expensive and time-consuming procedure when using a best known - direct method. This problem is particularly evident in hard-to-access populations, such as pediatric and geriatric. An alternative solution designed to determine reference intervals is using indirect methods, which are based on the results of studies performed for diagnostic purposes in outpatients and inpatients and archived in digital medical databases, which may be subjected to statistical anainical practice.
Acute myeloid leukemia (AML) is an aggressive hematological malignancy caused by a variety of genetic abnormalities and epigenetic dysregulation. The incidence of AML is strongly related to age, with the highest incidence rates being in older adults. The loss of function mutations in BCOR and BCORL1 genes have been identified in AML. BCL6 corepressor (BCOR) and BCL6 corepressor like 1 (BCORL1) are important epigenetic regu-lators as a member of Polycomb repressive complex 1 (PRC1.1), involved in histone modification processes.

We analyzed the BCOR and BCORL1 mRNA expression in 74 adult and 22 pediatric patients with AML by Real-Time quantitative PCR in this study.

Our results indicated that both BCOR and BCORL1 mRNA expressions decrease with age (p = 0.009 and p = 0.008, respectively) and there is a positive correlation between BCOR and BCORL1 mRNA expression (p < 0.001). BCOR and BCORL1 mRNA expressions were not significantly different in both adult and pediatric patients with AML compared to control (p > 0.05).

Our findings indicate that expression of BCOR and BCORL1 mRNA are down-regulated with age. The increase in AML incidence with age suggests that age-associated BCOR and BCORL1 down-regulation might potentially contribute to age-related epigenetic alterations and form a predisposing condition for the development of elderly AML.
Our findings indicate that expression of BCOR and BCORL1 mRNA are down-regulated with age. The increase in AML incidence with age suggests that age-associated BCOR and BCORL1 down-regulation might potentially contribute to age-related epigenetic alterations and form a predisposing condition for the development of elderly AML.
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