Notes

Speedy, multiplexed recognition in the let-7 miRNA family members making use of γPNA amphiphiles within micelle-tagging electrophoresis.
Central venous access is an essential aspect of critical care for pediatric patients. In the critically ill pediatric population, image-guided procedures performed at the bedside expedite care and may reduce risks and logistical challenges associated with patient transport to a remote procedure suite such as interventional radiology. We describe our institutional technique for ultrasound-guided tunneled femoral venous access in neonates and infants and provide technical pearls from our experience, with an intended audience including specialists performing point-of-care ultrasound-guided procedures as well as interventional radiologist making their services available in the intensive care unit.Background According to the World Health Organization (WHO), the outbreak of coronavirus disease in 2019 (COVID-19) has been declared as pandemic and public health emergency that infected more than 5 million people worldwide at the time of writing this protocol. Strong evidence for the burden, admission, and outcome of COVID-19 has not been published in Africa. Therefore, this protocol will be served as a guideline to conduct a systematic review and meta-analysis of the burden, admission, and outcome of COVID-19 in Africa. Methods Published and unpublished studies on the burden, admission, and outcome of COVID-19 in Africa and written in any language will be included. Databases (PubMed / MEDLINE, Google Scholar, Google, EMBASE, Web of Science, Microsoft Academic, WHO COVID-19 database, Cochran Library, Africa Wide Knowledge, and Africa Index Medicus) from December 2019 to May 2020 will be searched. Two independent reviewers will select, screen, extract data, and assess the risk of bias. The proportion will be measured using a random-effects model. Subgroup analysis will be conducted to manage hetrogeinity. The presence of publication bias will be assessed using Egger's test and visual inspection of the funnel plots. This systematic and meta-analysis review protocol will be reported per the PRISMA-P guidelines. Conclusion This systematic review and meta-analysis protocol will be expected to quantify the burden, admission, and outcome of COVID-19 in Africa. Systematic review registration This protocol was submitted for registration with the International Prospective Register of Systematic Reviews (PROSPERO) in March 2020 and accepted with the registration number CRD42020179321(https//www.crd.york.ac.uk/PROSPERO).Rates of attrition from child trauma-focused treatment are high, yet few predictors of premature termination are known. Caregiver-child symptom discrepancies are common in this population and have been related to treatment outcome. However, research has not examined whether caregiver-child symptom concordance is associated with attrition. The aim of the study was to determine whether pretreatment caregiver-child symptom agreement predicted premature termination from trauma-focused treatment. Two hundred and sixty-nine treatment-seeking children ages 8 to 12 (M = 9.97, SD = 1.49; 64.7% female, 51.3% Black) and their non-offending caregivers were included in the study. learn more Two operational definitions of attrition are as follows (a) clinician-rated dropout, and (b) whether the child received an adequate dose of treatment (i.e., 12 or more sessions), which were used to more thoroughly examine premature termination. Rates of attrition were high (68.1% clinician-rated premature termination, 37.4% received inadequate dose). Levels of symptom concordance between caregivers and children were low across symptom difficulties (intraclass correlations = .003-.16). Lower levels of discordance for posttraumatic stress symptoms (PTSS) were associated with an increased likelihood of receiving an adequate dose of treatment (odds ratio [OR] = 1.03). Nonetheless, unexpectedly, higher levels of caregiver-child discordance for anxiety symptoms at pretreatment predicted both clinician-rated treatment completion and adequate dose (ORs = .97, .96, respectively). However, caregiver's and children's perceptions of children's trauma-related difficulties may not converge, and thus, both reports are important to assess. Symptom disagreement regarding PTSS may help identify families at risk for attrition.The study of intimate partner violence (IPV) has evolved into more complex and integrative models to better understand the explanatory mechanisms of IPV perpetration. Nevertheless, integrative models rarely permit an in-depth analysis of a large number of relevant variables, and more specific approximations are required as basis of them. In this sense, the individual approach is the most productive research line. From the individual perspective and using data from imprisoned male offenders, the present study aimed to analyze the potential mediating role of alcohol dependence in the association of Cluster B personality traits and psychological IPV. The sample included 196 male inmates of the Penitentiary Center of Villabona (Asturias, Spain). Structural equation modeling was used to test the hypothetical and alternative models. First, model results only showed an indirect effect of antisocial and borderline personalities mediated by alcohol dependence. Nevertheless, fitting of the model to the data was poor. S.Pseudomonas protegens CHA0, known as plant-growth-promoting rhizobacterium, showed positive chemotactic responses toward proteinaceous L-amino acids. Genomic analysis revealed that P. protegens CHA0 possesses four putative chemoreceptors for amino acids (designated CtaA, CtaB, CtaC, and CtaD, respectively). Pseudomonas aeruginosa PCT2, a mutant defective in chemotaxis to amino acids, harboring a plasmid containing each of ctaA, ctaB, ctaC, and ctaD showed chemotactic responses to 20, 4, 4, and 11 types of amino acids, respectively. To enhance chemotaxis toward amino acids, we introduced the plasmids containing ctaA, ctaB, ctaC, or ctaD into P. protegens CHA0. By overexpression of the genes, we succeeded in enhancing chemotaxis toward more than half of the tested ligands. However, unexpectedly, the P. protegens CHA0 transformants showed unchanged or decreased responses to some amino acids when compared to wild-type CHA0. We speculate that alternation of expression of a chemoreceptor may affect the abundance of other chemoreceptors.
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