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Hypoxia tolerance in two amazon online cichlids: mitochondrial respiratory and cellular metabolic process alterations are results of species ecological choices and submission.
The objective of this study was to evaluate placental development during late gestation (day 100) between Chinese Meishan (CM; n = 7) and White crossbred (WC; n = 5) gilts following intrauterine crowding induced by unilaterally hysterectomy-ovariectomy. Gross placental morphology and areolae density as well as histological morphology (i.e., folded bilayer and placental stroma) were analyzed using computer-assisted morphometry for placentas of the smallest and largest fetuses within each litter. There was a breed by fetal size interaction (P  less then   0.01) for areolae density in which placentas for large CM fetuses had greater areolae density compared to small CM fetuses, but the density of areolae was greater for CM fetuses compared to WC fetuses, irrespective of fetal size. The width of the folded bilayer was greater (P  less then   0.01) in placentas for WC gilts compared to CM gilts, irrespective of fetal size. Placentas for small fetuses had greater (P  less then   0.01) folded bilayer width compared to large fetuses, irrespective of breed. The placental stromal width was greater (P less then 0.01) in placentas for large fetuses compared to small, irrespective of breed. The difference between stromal width in placentas between divergent-sized littermates, however, was greater (P =  0.05) in WC gilts compared to CM gilts, indicating there was a limited response to intrauterine crowding in CM gilts. These results indicate there is an altered placental development during late gestation in CM compared to WC gilts, thus, there are likely different mechanisms for responding to intrauterine crowding between breeds.Poor processing stability has been cited as the fatal shortcoming of the up-flow anaerobic sludge blanket (UASB) reactor treating starch wastewater (SW). In this study, the SW treatment performance in a one-stage UASB reactor and a pre-acidification equipped UASB process were evaluated together with the microbial dynamics. The results revealed that the pre-acidification provided improvements in terms of the substrate utilization diversity and the stability of the microbial community structure on the UASB reactor. Anaerolineaceae/Methanosaeta was the core functional microbiota in the pre-acidification equipped UASB reactor, indicated the superior abilities on the acetogenic methanogenesis of granules. The genus of Methanobacterium, a hydrogenotrophic methanogen was dominant in the archaeal community in the one-stage UASB reactor. The granules performed very strong hydrogen affinity in methane production, a small amount of propionate was detected in the effluent. These were abnormal, which suggested the high hydrogen turn-over rate in the one-stage UASB reactor.To investigate the effect of microplastics (MPs) particles in vermicomposting, polyethylene (PE) particles added into sludge. Results showed that the vermicomposting with high MPs addition obtained lower removal efficiencies for organics than the vermicomposting with low MPs addition. The content of DOC and NH4+-N in M4 reactor (with the highest MPs addition) at 80 days was 8.4 mg/kg and 74.2 mg/kg, respectively. The pH, C/N, electrical conductivity (EC), and germination index (GI) results showed that the addition amount of MPs was directly proportional to the negative effect of composting. The negative effect mainly occurred after 20 days of composting. High MPs addition resulted in apparent oxidative stress and neurotoxicity on earthworm, the values of catalase (CAT) and acetylcholine esterase (AChE) in M4 reactor increased by 2.03 times and 1.60 times. The bacteria in M4 were more barren and lower in terms of diversity.Polyhydroxyalkanoates (PHA) are microbial polyesters which, apart from their primary storage role, enhance the stress robustness of PHA accumulating cells against various stressors. PHA also represent interesting alternatives to petrochemical polymers, which can be produced from renewable resources employing approaches of microbial biotechnology. During biotechnological processes, bacterial cells are exposed to various stressor factors such as fluctuations in temperature, osmolarity, pH-value, elevated pressure or the presence of microbial inhibitors. This review summarizes how PHA helps microbial cells to cope with biotechnological process-relevant stressors and, vice versa, how various stress conditions can affect PHA production processes. The review suggests a fundamentally new strategy for PHA production the fine-tuned exposure to selected stressors, which might be used to boost PHA production and even to tailor their structure.Cell immobilization was used to enrich and retain functional bacteria within partial denitrification-Anammox (PD/A) process to achieve its fast start-up for the first time. To do so, residue sludge and Anammox sludge were immobilized in poly (vinyl alcohol)/sodium alginate (PVA/SA) gel for PD cultivation and Anammox bacteria inoculation, respectively. Stable PD with NO3--N to NO2--N transformation ratio (NTR) of 72.0% was achieved within 13 days at 25 °C and successfully combined with Anammox on 14th day. The hydrous porous PVA/SA gel matrix played the role of extracellular polymeric substance (EPS) and thus protected the microbes against low temperature. Satisfactory nitrogen removal rate (NRR) (301.6 ± 6.1 g N/(m3·d)) was achieved even when temperature decreased to 13 °C. The contribution of nitrogen removal via Anammox was as high as 77.10%. Abundance of Thauera and Candidatus Kuenenia increased from 0.9% and 1.1% to 30.6% and 2.1%, respectively.Leucine dehydrogenase (LDH) is widely used in the preparation of L-2-aminobutyric acid (L-2-ABA), however its wide application is limited by 2-ketobutyric acid (2-OBA) inhibition. Firstly, a novel high-throughput screening method of LDH was established, specific enzyme activity and 2-OBA tolerance of Lys72Ala mutant were 33.3% higher than those of the wild type. Subsequently, we constructed a single cell comprised of ivlA, EsldhK72A, fdh and optimized expression through fine-tuning RBS intensity, so that the yield of E. check details coli BL21/pET28a-R3ivlA-EsldhK72A-fdh was 2.6 times higher than that of the original strain. As a result, 150 g L-threonine was transformed to 121 g L-2-ABA in 5 L fermenter with 95% molar conversion rate, and a productivity of 5.04 g·L-1·h-1, which is the highest productivity of L-2-ABA currently reported by single-cell biotransformation. In summary, our research provided a green synthesis for L-2-ABA, which has potential for industrial production of drug precursors.
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