Notes

[Calorie as well as macronutrient intake in the 1st few months soon after large volume surgery].
The death of microalgae plays an important role in ocean energy flow and material circulation. The existing methods for the identification of dead and living microalgae cells all have defects such as the need for staining and pre-treatment. In this work, a label-free method to identify living and dead algae cells based on digital holography microscopy and machine learning was designed. At the stage of model training, ten feature vectors were extracted from the holograms, and twelve classification models of machine learning algorithm were trained. Compared with the staining method results, the accuracy of this method can reach 94.8%. At the stage of field verification, the death rate calculated by this method was also consistent with staining method. The method proposed in this paper provides a new method for the study of marine microalgae death which has the advantages of label-free, non-invasive, high accuracy and potential for in-situ application.This study is the first study to determine beach litter pollution in the Eastern Mediterranean region of Turkey. The pollution of beach litter were seasonally evaluated in a selected beach in the East Mediterranean Sea between August 2019 and April 2020. Beach litter survey was carried out seasonally according to 'Guidance on Monitoring of Marine Litter in European Seas' published by European Marine Strategy Framework Directive Technical Subgroup on Marine Litter. During the study, a total of 10.717 items and 229.29 kg were collected. The average litter abundance was 2.679 ± 0.418 items/m2 and 57.326 ± 7.165 g/m2. The results indicated that the most common type of litter were plastic and cloth/textile (84%) followed by paper/cardboard (56%), the unidentifiable (51%). Litter density showed significant seasonal differences with the highest in summer (P less then 0.05). Akyatan beach was classified as extremely dirty according to Clean Coast Index. It was observed that the litter in the region averagely consisted mostly of mixed packaging litter (23.75%), domestic litter (20%) and unclassified litter (16.2%) items. Our results show that the coast of Akyatan Lagoon exposed to a significant amount of marine litter pollution originated from land-based sources. This study provides further evidence that there is a significant litter pollution in the East Mediterranean Sea and that the main component are plastics, which pose a great treat to Mediterranean Sea environment.This study evaluated the protective effect of TiF4 varnish, after pre-treatment with proantocyanidin or chlorhexidine, on the progression of erosive dentin loss (EDL), under the presence or absence of the demineralized organic matrix (DOM). Bovine root dentin samples were eroded for 30 min (0.1% citric acid, pH 2.5) and the loss was measured by profilometry. Half of them were subjected to the DOM removal using collagenase for 4 days, while the other half remained immersed in water. The removal of DOM was checked by profilometry. Samples were divided into 24 groups (n = 15) according to the factors 1- With or without DOM; 2- Pre-treatment with 0.012% chlorhexidine gel, 10% proanthocyanidin gel or untreated for 1 min; 3-Final Treatment with TiF4 varnish, NaF varnish, placebo varnish or untreated for 6 h. The samples were submitted to a pH cycling for 5 days 0.1% citric acid (4 × 90s/day) and artificial saliva between the challenges. The final profile was obtained for the calculation of EDL (μm, three-way ANOVA/Tukey test). When DOM was preserved, the EDL was lower compared to the condition without DOM (7.08 ± 4.03 μm and 9.80 ± 3.79 μm, p less then 0.001, respectively), regardless of the treatments. The pre-treatment had no influence on the progression of EDL (p = 0.637), while the final treatment (TiF4 varnish only, 6.77 ± 4.08 μm) was effective in reducing the progression of EDL (NaF varnish 9.52 ± 4.02 μm; Placebo varnish 8.64 ± 4.06 μm and no treatment 8.80 ± 3.95 μm). It can be concluded that DOM has important protective effect on the progression of EDL. TiF4 was the unique treatment capable of reducing EDL progression, regardless of the pre-treatment of DOM.Reductive dechlorination, an efficient pathway for complete removal of organic chlorinated pollutants (OCPs), is commonly reported to be coupled to oxidation of methane (CH4) or methanogenesis in anaerobic environments. However, the relationship between dechlorination and CH4-associated bioprocesses is unclear. Based on the hypothesis that CH4 supplementation could facilitate OCP dechlorination, we investigated the role of CH4-associated bioprocesses in the self-purification of flooded lindane-spiked paddy soils. Four treatments were conducted for up to 28 days sterilized soil (S), sterilized soil + CH4 (SC), non-sterilized soil (NS), and non-sterilized soil + CH4 (NSC). Results indicated that both sterilization and addition of CH4 promoted lindane degradation and CH4 emissions in the flooded paddy soils. In the NS treatment, lindane had the lowest degradation rate when CH4 emissions were barely detected; while in the SC treatment, lindane had the highest degradation rate when CH4 achieved its highest emissions from anaerobic soil. Also, sterilization led to microbial diversity loss and functional recession, but increased ferrous ion [Fe(II)] concentrations compared to non-sterilized soils. Methanogenic communities and mcrA gene recovered faster than the majority of microorganisms (e.g., Fe bacteria, Bdellovibrionaceae, Rhizobiaceae, Dehalogenimonas) or functional genes (e.g., Dhc, Geo, narG, nirS). Collectively, we assume the enhanced removal of lindane may partly be due to both abiotic dechlorination promoted by chemical Fe redox processes and methanogenesis-derived biotic dechlorination. Revealing the coupling between dechlorination and CH4-associated bioprocesses is helpful to resolve both pollution remediation and mitigation of CH4 emissions in anaerobic contaminated sites.In the present study, starch based ZnO nancomposite (CSt-ZnO) was synthesized for the efficient removal of Pb(II) ions from aqueous medium. The structure and morphology of CSt-ZnO nancomposite was characterized using SEM, FTIR, TGA, BET, XPS and zeta potential measurements. The effect of contact time, pH, temperature and initial concentration of Pb(II) on the adsorption was studied. The optimum parameters for maximum Pb(II) removal were time-120 min; pH-6; temperature-318 K and Co-20 ppm. PIK-75 price The maximum Langmuir adsorption capacity of CSt-ZnO nancomposite was 256.4 mg/g at 298 K. With increasing the temperature from 298 K to 318 K, the maximum adsorption quantity (qm) was improved from 256.4 to 476 mg/g which showed the endothermic nature of Pb(II) adsorption on CSt-ZnO nanocomposite. The sorption isotherm and kinetics model fitting studies, confirmed that data fit well to Freundlich isotherm and pseudo-first-order kinetics models, respectively. Thermodynamic studies inferred a spontaneous and endothermic nature of adsorption.
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