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Finger somatotopy will be maintained right after tetraplegia however drops with time.
The structural health monitoring of power transmission towers (PTTs) has drawn increasing attention from researchers in recent years; however, no long-term monitoring of the dynamic parameters of PTTs has previously been reported in the literature. This study performed the long-term monitoring of an instrumented PTT. An automated subspace identification technique was used to extract the dynamic parameters of the PTT from ambient vibration measurements taken over approximately ten months in 2017. Ten target modal frequencies were selected to explore the effects of environmental factors, such as temperature and wind speed, as well as the root-mean-square (RMS) acceleration response of the PTT. Variations in the modal frequencies of approximately 2% to 8% were observed during the study period. In general, among the environmental factors, the temperature was found to be the primary cause of decreases in the modal frequencies, except in the case of some of the higher modes. Typhoon Nesat, which affected the PTT on July 29th, 2017, seems to have decreased the modal frequencies of the PTT, especially for the higher modes. This reduction in the modal frequencies seems to have lasted for approximately two and a half months, after which they recovered to their normal state, probably due to a seasonal cool down in temperature. The reduction percentages in the modal frequencies due to Typhoon Nesat were quantified as approximately -0.89% to -1.34% for the higher modes, but only -0.07% to -0.46% for the remaining lower modes. Although the unusual reductions in the modal frequencies are reported in this study, the reason for this phenomenon is not clear yet. Further studies would be required in the future in order to find the cause.Vision-based systems operating outdoors are significantly affected by weather conditions, notably those related to atmospheric turbidity. Accordingly, haze removal algorithms, actively being researched over the last decade, have come into use as a pre-processing step. Although numerous approaches have existed previously, an efficient method coupled with fast implementation is still in great demand. click here This paper proposes a single image haze removal algorithm with a corresponding hardware implementation for facilitating real-time processing. Contrary to methods that invert the physical model describing the formation of hazy images, the proposed approach mainly exploits computationally efficient image processing techniques such as detail enhancement, multiple-exposure image fusion, and adaptive tone remapping. Therefore, it possesses low computational complexity while achieving good performance compared to other state-of-the-art methods. Moreover, the low computational cost also brings about a compact hardware implementation capable of handling high-quality videos at an acceptable rate, that is, greater than 25 frames per second, as verified with a Field Programmable Gate Array chip. The software source code and datasets are available online for public use.The ability to quantitate a protein of interest temporally and spatially at subcellular resolution in living cells would generate new opportunities for research and drug discovery, but remains a major technical challenge. Here, we describe dynamic, high-sensitivity protein quantitation technique using NanoLuciferase (NLuc) tagging, which is effective across microscopy and multiwell platforms. Using collagen as a test protein, the CRISPR-Cas9-mediated introduction of nluc (encoding NLuc) into the Col1a2 locus enabled the simplification and miniaturisation of procollagen-I (PC-I) quantitation. Collagen was chosen because of the clinical interest in its dysregulation in cardiovascular and musculoskeletal disorders, and in fibrosis, which is a confounding factor in 45% of deaths, including those brought about by cancer. Collagen is also the cargo protein of choice for studying protein secretion because of its unusual shape and size. However, the use of overexpression promoters (which drowns out endogenous regulatory mechanisms) is often needed to achieve good signal/noise ratios in fluorescence microscopy of tagged collagen. We show that endogenous knock-in of NLuc, combined with its high brightness, negates the need to use exogenous promoters, preserves the circadian regulation of collagen synthesis and the responsiveness to TGF-β, and enables time-lapse microscopy of intracellular transport compartments containing procollagen cargo. In conclusion, we demonstrate the utility of CRISPR-Cas9-mediated endogenous NLuc tagging to robustly quantitate extracellular, intracellular, and subcellular protein levels and localisation.Sourdough fermentation of bakery products is a well-established and widespread technique to confer an added value to the resulting food. In recent decades, gluten-free raw materials have gained more attention due to the diffusion of food disorders such as coeliac disease, but, at the same time, they present difficult manipulation and scarce technological properties because of the absence of gluten. For this reason, the present work was aimed at selecting starter cultures for sourdough application that are isolated from fermentation of sorghum flour. Three isolates of Lactobacillus fermentum, Weissella cibaria, and Weissella confusa were selected for the following properties exopolysaccharide synthesis, acidification, CO2 production, and amylase activity. The investigated phenotypic characteristics were confirmed by genomic analyses, which also highlighted other potentially beneficial features for use in bakery products employment. These strains, together with bakery yeast, were used for bread preparation using sorghum and wheat flour and after 24 h of fermentation the resulting dough was analyzed to assess the improvement of its characteristics. The presence of lactic acid bacteria (LAB) had a great impact on the final dough, and the best preparation, from a rheological point of view, resulted in one made of sorghum and wheat flour with added LAB and bakery yeast, whose resulting characteristics were similar to all wheat flour doughs. The results of this study suggest a potential application of the selected starters in sorghum composite bread and should be validated with data from large-scale pilot tests conducted in industrial bakeries.
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