Notes

In season along with Parallel Cleistogamy within Rostrate Violets (Viola, subsect. Rostratae, Violaceae).
Therefore, this study provides a convenient approach to improve the nitrogen removal efficiency of municipal sewage with low C/N.This study aims at exploring the mechanism of fast-stimulating bioremediation of macro crude oil using matching Fenton pre-oxidation. The 80-day biodegradation experiment for soil S1 and S2, containing macro crude oil C19-C29 and C17-C29 respectively, was conducted after Fenton pre-oxidation with three concentrations of H2O2 (225 mM, 450 mM, and 900 mM). Experimental results indicated that the bioremediation efficiency of macro crude oil was up to 57.1% (8853 mg/kg, S1) and 64.4% (11,719 mg/kg, S2) for 80-day fast-stimulating bioremediation using matching Fenton pre-oxidation (450 mM H2O2), which was 1.8-2.6 times that (S1 22.2-37.1%; S2 36.1-39.6%) for slow-stimulating bioremediation using un-matching Fenton pre-oxidation. Furthermore, the high-throughput analysis revealed that genera Sedimentibacter, Caenispirillum, and Brevundimonas became the dominant bacteria after matching Fenton pre-oxidation. Meanwhile, the highest logarithmic growth rate of indigenous hydrocarbon degraders (IHD) was obtained (S1 64% and S2 60%) for fast-stimulating bioremediation. And the consumption of NH4+-N was up to 90% and 94% in S1 and S2 within 60 days for fast-stimulating bioremediation, approximately 1.4 and 2.2 times that (S1 65% and 62%; S2 47% and 41%) for slow-stimulating remediation. The results showed that the macro crude oil became the main carbon source for IHD for the fast-stimulating bioremediation, resulting in the rapid growth of IHD. Thus, this study provides a fast and efficient remediation technology for bioremediation of macro crude oil-contaminated soils.In recent years, surfactant-enhanced aquifer remediation (SEAR) has attracted increasing interest duo to the high efficiency of removing non-aqueous phase liquids (NAPLs) from aquifer. A thorough understanding of SEAR is necessary for its successful implementation in field remediation. This paper reviewed the SEAR technology in a comprehensive way based on the recent research advances. Firstly, an overview of the basic processes and mechanisms underlying the technology was presented. Secondly, applications of SEAR and the factors that influence the performance were summarized. Thirdly, the key limitations of SEAR, which are downward migration of dense-NAPLs, secondary pollution of surfactants, adsorptive, precipitative and partitioning loss of surfactants, and heterogeneity of the aquifer, were reviewed. Finally, the recent advances in modifying SEAR to overcome the limitations were discussed in detail. The review will promote our understanding of SEAR technology and provide some useful information to improve the performance of SEAR in applications.Laccase, a unique class of multicopper oxidase, presents promising potential as a biocatalyst in many industrial and biotechnological applications. Recently, it has been significantly applied in many metal-polluted sites due to its Manganese (Mn)-oxidation ability. Here, we demonstrate the Mn(II)-oxidase activity of laccase obtained from Bacillus sp. GZB. The CotA gene of GZB was transformed in E. 8-Bromo-cAMP coli BL21 and overexpressed. The purified laccase (LACREC3-laccase) displayed the absence of a peak at 610 nm that is usually found in blue-laccase. Further, the LACREC3-laccase exhibited high activity and stability at different pH and temperatures with substrates 2, 2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonate) and syringaldazine, respectively. It also functioned in the presence of various metals and enzyme inhibitors. Most notably, LACREC3-laccase formed insoluble brown Mn(III)/Mn(IV)-oxide particles from Mn(II) mineral, exhibiting its Mn(II)-oxidase activity. In addition to native polyacrylamide gel electrophoresis and buffer test, we developed an 'agarose gel plate' assay to evaluate Mn(II) oxidation activity of laccase. Furthermore, using the leucoberbelin blue assay, a total of 44.45 ± 0.45% Mn(IV)-oxides were quantified, in which 5.87 ± 0.61% autoxidized after 24 h. The Mn(II) oxidation mechanisms were further predicted by trapping Mn(III) using pyrophosphate during Mn(II) to Mn(IV) conversion by LACREC3-laccase. Overall, the laccase of GZB has excellent activity and stability plus an ability to oxidize Mn(II). This study is the first report on a non-blue laccase, exhibiting Mn(II)-oxidase activity. Thus, it offers a novel finding of the Mn(II) oxidation processes that can be a valuable way of Mn(II)-mineralization in various metal-polluted environments.Human papillomavirus (HPV) infections are common sexually-transmitted diseases among reproductive-aged women with increasing concern. Until now, there are no prior study about the association between HPV infections and ambient air pollution. This study aimed to explore the relationship between short-term exposure to ambient pollutants and daily outpatient visits for HPV infections in China. Data of daily outpatient visits for HPV infections were obtained from January 1, 2014 to December 31, 2018 (1826 days). Over-dispersed Poisson generalized additive models were applied by adjusting weather conditions and day of the week. We identified a total of 39,746 cases for HPV infections. A 10 μg/m3 increase of PM10, PM2.5, SO2, and NO2 or a 0.1 mg/m3 rise of CO in concurrent day (lag 0) concentrations was related to an elevation of 0.822% (95% Cl 0.282%, 1.36%), 1.05% (95% Cl 0.280%, 1.81%), 5.72% (95% Cl 1.79%, 9.65%), 5.02% (95% Cl 3.45%, 6.60%), and 2.40% (95% Cl 1.43%, 3.37%) in daily outpatient-visits for HPV infections, respectively. The association was more significant in those women aged 41 or over. As for 10 μg/m3 increase of O3, a -1.33% (95% Cl -2.13%, -0.530%) change was observed on the lag 03 and such effects appeared to be more obvious in the aged 18-40 group. Our results provided the first evidence that short-term exposure to ambient pollutants was related to, which may be indirectly, the increased risk of HPV infections while O3 may act as a "protective" factor.Halogenated disinfection byproducts (DBPs) are formed during chlorine disinfection of drinking water. The complicated natural organic matter in source water causes the formation of an even more complicated mixture of DBPs. To evaluate the toxicity of a DBP mixture in a disinfected water sample, the sample needs to be pretreated in order to attain an observable acute adverse effect in the toxicity test. During sample pretreatment, volatile DBPs including trihalomethanes, haloacetonitriles and haloketones may be lost, which could affect the toxicity evaluation of the DBP mixture. In this study, we intentionally prepared "concentrated" simulated drinking water samples, which contained sufficiently high levels of volatile and nonvolatile DBPs and thus enabled directly evaluating the toxicity of the DBP mixtures without sample pretreatment. Specifically, the natural organic matter and bromide concentrations and the chlorine dose in the concentrated water samples were 250 times higher than those in a typical drinking water sample.
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