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Oil palm (Elaeis guineensis) trunk chips were processed by steam explosion under different steam conditions, followed by alkaline extraction and fermentation to produce efficient lignocellulosic ethanol as sustainable alternative energy resource. The optimum condition of steam explosion was attained at 210°C for 4 min (α-cellulose 58.83% and lignin 27.12%). Taguchi 3 factor design [(sodium hydroxide concentration (NaOH), temperature and time)] was performed to optimize alkaline extraction. The optimum condition at 15% NaOH, 90°C for 60 min gave highest percentage α-cellulose 87.14% and lowest percentage of lignin 6.13%. Simultaneous saccharification and fermentation (SSF) involved 10% dry weight pretreated fibers, Celluclast 1.5L (15 FPU /gram substrate), Novozyme 188 (15 IU/gram substrate) and Saccharomyces cerevisiae SC90. The highest ethanol concentration (CP) produced during SSF was 44.25 g/L. Nonetheless, pre-hydrolysis simultaneous saccharification and fermentation gave 31.22 g/L (CP). All results suggested that optimized two step pretreatment produced efficient ethanol.Dark fermentation is a technically feasible technology for achieving carbon dioxide-free hydrogen production. This review presents the current findings on continuous hydrogen production using dark fermentation. Several operational strategies and reactor configurations have been suggested. The formation of attached mixed-culture microorganisms is a typical prerequisite for achieving high production rate, hydrogen yield, and resilience. To date, fixed-bed reactors and dynamic membrane bioreactors yielded higher biohydrogen performance than other configurations. The symbiosis between H2-producing bacteria and biofilm-forming bacteria was essential to avoid washout and maintain the high loading rates and hydrogenic metabolic flux. Recent research has initiated a more in-depth comparison of microbial community changes during dark fermentation, primarily with computational science techniques based on 16S rRNA gene sequencing investigations. Future techno-economic analysis of dark fermentative biohydrogen production and perspectives on unraveling mitigation mechanisms induced by attached microorganisms in dark fermentation processes are further discussed.
There is a lack of studies simultaneously evaluating the impact of structural and functional atherosclerosis on cognition. We investigated the long-term predictive and interaction effects of structural and functional carotid atherosclerosis markers on future cognitive decline.
Five hundred and twenty-eight middle-aged participants enrolled in the carotid atherosclerosis examination in Kaohsiung Atherosclerosis Longitudinal Study (KALS) between 2006 and 2009 were tested for cognition between 2016 and 2019. The Montreal Cognitive Assessment (MoCA) was used for the cognitive test. Baseline structural atherosclerosis was assessed by carotid intima-media thickness (cIMT) and plaque, whereas functional atherosclerosis was evaluated by carotid stiffness (β, Ep, and pulse wave velocity). Participants in the top quartile of cIMT and those with plaques were considered to have advanced structural atherosclerosis, whereas participants with all three stiffness parameters in the top quartile were defined to have advanced functional atherosclerosis.
The mean participant age at baseline was 53.88±8.37 years. Each case of advanced structural atherosclerosis and advanced functional atherosclerosis was associated with low 10-year MoCA scores with p<0.001 and p=0.03, respectively. An interaction effect was observed between structural and functional atherosclerosis on the MoCA score 10 years later (p=0.02). Participants with both advanced structural and functional markers showed a marked impact on future cognitive function, especially executive and language domains.
Carotid atherosclerosis in middle-aged individuals can predict their cognitive function in 10 years. Integrated information regarding both arterial wall and stiffness could help improve the predictive power for cognitive decline.
Carotid atherosclerosis in middle-aged individuals can predict their cognitive function in 10 years. Integrated information regarding both arterial wall and stiffness could help improve the predictive power for cognitive decline.
Lipoprotein particle size is associated with increased atherosclerosis and cardiovascular disease risk. Certain lifestyle behaviours may be cardioprotective. We examined lipoprotein particle size and concentration relationships with a protective lifestyle behaviour (PLB) score.
This was a cross-sectional analysis of 2045 middle-to older-aged adults. Lipoprotein particle subclass size and concentrations were determined using nuclear magnetic resonance spectroscopy. Five protective behaviours included never smoking, moderate alcohol intake, moderate to vigorous physical activity, a high-quality diet (upper 40% Dietary Approaches to Stop Hypertension score) and a normal body mass index (BMI) (18.5-24.9kg/m
). Linear and logistic regression analyses tested individual protective behaviour and PLB score associations with lipoprotein subclasses.
Individual behaviour associations varied according to lipoprotein subclass, with normal BMI showing the greatest number of significant relationships. Logistic regresslts, highlighting the importance of lifestyle promotion in healthy ageing.
Children with a growth trajectory of overweight have higher levels of cardiovascular disease (CVD) risk factors than children with a normal-weight trajectory. HIF inhibitor However, less is known about how trajectories of body mass index (BMI) across the rest of the BMI spectrum relate to CVD risk factors and whether adult BMI affects these associations. Our aim was to examine associations between childhood BMI trajectories and adult CVD risk factors.
We included 2466 individuals with childhood weights and heights (ages 6-14) from the Copenhagen School Health Records Register and adult CVD risk factors (ages 20-81) from the Copenhagen City Heart Study. Associations between childhood BMI trajectories identified by latent class modelling and CVD risk factors were examined using generalized linear regression analyses with and without adjustment for adult BMI. Normal-weight and overweight were defined by growth references from the Centers for Disease Control and Prevention.
We identified four childhood trajectories within the normal-weight spectrum and one trajectory of overweight.
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