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Corrigendum: PKM2-Induced your Phosphorylation of Histone H3 Leads to EGF-Mediated PD-L1 Transcription inside HCC.
In this study, a new adsorbent, polyvinyl alcohol (PVA) and graphene oxide (GO), was prepared, characterized and used for the removal of Sr2+ from aqueous solution. In PVA/GO composite, the inter-lamellar spacing of adjacent GO layers was dramatically enlarged due to the intercalation of PVA molecules, such a unique architecture significantly mitigated the aggregation of GO layers, which facilitated the accessible exposure of active sites and the mass transfer of strontium ions (Sr2+), thus enhancing the adsorption capacity toward Sr2+. The adsorption of Sr2+ by PVA/GO composite conformed to the pseudo second-order kinetic model (R2 = 0.9994), the Langmuir model (R2 = 0.9042), and the Freundlich model (R2 = 0.9598). The complexation interaction between Sr2+ and oxygen atoms/π-electron domain of PVA/GO composite was primarily responsible for the adsorption mechanism, based on the characterization results of X-ray photoelectron spectroscopy (XPS), scanning electron microscope equipped with energy dispersion spectroscopy (SEM-EDS) and powder X-ray diffraction (PXRD).The coordination between photosynthesis and aerobic respiration under mixotrophic cultivation can make a difference to the growth and biochemical composition of microalgae. However, the response of carbon metabolism to carbon source composition under mixotrophic microalgae cultivation has not been well studied. In this study, the synergistic effects of inorganic carbon (IC) and organic carbon (OC) supply on the growth and carbon metabolism of Chlorella vulgaris under mixotrophic cultivation were investigated. The increase of the proportion of HCO3- had a positive effect on the expression of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), which promoted the biomass production and carbon fixing. The activity of citrate synthase was attenuated with the increase of IC/OC ratio, indicating that the energy needed for the biomass production in groups with high IC/OC ratio was contributed by photoreaction. Biochemical analysis showed that CO32- was more efficient than HCO3- for carbohydrate and lipid accumulation of Chlorella vulgaris, and the highest amount of carbohydrate (30.2%) and lipid (35.8%) was recorded with the combined use of CO32- and glucose. The results could provide a new perspective on carbon metabolism and enzyme regulation in mixotrophic microalgae cultivation.A novel ecological-microbial fuel cell (E-MFC) was constructed based on the mutualistic symbiosis relationship among wetland plants Ipomoea aquatic, benthic fauna Tubifex tubifex (T. tubifex) and microorganisms. The maximum power densities of sediment MFC (S-MFC), wetland plant MFC (WP-MFC) and E-MFC were 6.80 mW/m2, 10.60 mW/m2 and 15.59 mW/m2, respectively. Ipomoea aquatic roots secreted organic matter as electricigens' fuel for electricity generation, while T. tubifex decomposed decaying leaves and roots into soluble organic matter and plant nutrients, forming a co-dependent and mutually beneficial system, which was conducive to bioelectricity production. The E-MFC obtained the highest nitrogen removal, and the removal efficiencies of NH4+-N and NO3--N were 90.4% and 96.5%, respectively. check details Hydraulic retention time (HRT), cathodic aeration and T. tubifex abundance had significant effects on E-MFC power generation. The performeance boost of E-MFC was closely related to anodic microbial community change caused by the introduction of T. tubifex.The autotrophic iron-depended denitrification (AIDD), triggered by microelectrolysis, was established in the microelectrolysis-assistant up-flow anaerobic sludge blanket (MEA-UASB) with the purpose of low-strength coal gasification wastewater (LSCGW) treatment while control UASB operated in parallel. The results revealed that chemical oxygen demand (COD) removal efficiency and total nitrogen (TN) removal load at optimum current (2.5 A/m3) in MEA-UASB (83.2 ± 2.6% and 0.220 ± 0.010 kg N/m3·d) were 1.42-fold and 1.57-fold higher than those (58.5 ± 2.1% and 0.139 ± 0.011 kg N/m3·d) in UASB, verifying that AIDD and following dissimilatory iron reduction (DIR) process could offer the novel pathway to solve the electron donor-deficient and traditionally denitrification-infeasible problems. High-throughput 16S rRNA gene pyrosequencing shown that iron-oxidizing denitrifiers (Thiobacillus and Acidovorax species) and iron reducing bacteria (Geothrix and Ignavibacterium speices), acted as microbial iron cycle of contributors, were specially enriched at optimum operating condition. Additionally, the activities of microbial electron transfer chain, electron transporters (complex I, II, III and cytochrome c) and abundance of genes encoding important enzymes (narG, nirK/S, norB and nosZ) were remarkably promoted, suggesting that electron transport and consumption capacities were stimulated during denitrification process. This study could shed light on better understanding about microelectrolysis-triggered AIDD for treatment of refractory LSCGW and further widen its application potential in the future.Organic acids-assisted phytoremediation is a promising strategy to remove metal pollutants from the soil. However, few reports have focused on the mechanisms of organic acids promoting the uptake of heavy metals by hyperaccumulators. In this study, 5 types of organic acids, namely polybasic carboxylic acids, acidic amino acids, acidic plant growth regulators, phosphoric and gluconic acids, were comprehensively investigated the effects on the solubility of Cd and Pb in the soil along with their uptake by Cd hyperaccumulator Solanum nigrum L. The results indicated that the addition of Hydroxyethylidene-1,1-diphosphonic acid (HEDP) and d-Gluconic acid (D-GA) effectively extracted the most of acid-extractable and some of reducible and oxidizable fractions of Cd and Pb in the soil, with the extraction rates of 64.8% and 34.4% for total Cd and 53.6% and 30.0% for total Pb, respectively. HEDP and D-GA significantly increased the accumulations of Cd (57.1% and 35.0%) and Pb (43.4% and 31.9%) by S. nigrum without the inhibition of its biomass, making the great removal efficiencies of Cd (1.35% and 1.16%) and Pb (0.039% and 0.036%) from the soil. The enhanced phytoremediation efficiency of S. nigrum was due to the increase of the extractable Cd and Pb in the rhizosphere but little changes of soil pH and enzyme activities (catalase and urease). Among all of organic acids, HEDP may be an alternative to EDTA because of its characteristics of environmental friendliness and high efficiency.
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