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Causes of make use of problems throughout ventilation units -- Systematic assessment.
This study studied the change of functionalities in the biochar formed in pyrolysis of poplar wood in a wide range of temperature. The in situ Diffuse Reflectance Infrared Fourier transform spectroscopy characterization indicated that aldehydes and ketones functionalities formation initiated at 100 °C, dominated at 300 to 500 °C. Carboxyl group was less stable than carbonyls. Cellulose crystal in poplar decomposed slightly at 300 °C and significantly at 350 °C. The temperature from 250 to 350 °C significantly affected biochar yields, while the drastic fusion of the ring structures in biochar occurred from 550 to 650 °C, making biochar more aliphatic while less more aromatic. High pyrolysis temperature also created more defective structures in the biochar and favored the absorption of the CO2 generated during the pyrolysis. The results provide the reference information for understanding the structural configuration and evolution of the functionalities during in pyrolysis of poplar biomass. Free nitrous acid based pretreatments are novel and effective chemical strategies for enhancing waste activated sludge solubilization. In this study, the synergetic effects of the combined free nitrous acid and electrochemical pretreatment on sludge solubilization and subsequent methane productivity were evaluated. The results indicated that pretreatment with 10 V plus 14.17 mg N/L substantially enhanced sludge solubilization, with the highest soluble chemical oxygen demand concentration of 3296.7 mg/L, 25.6-time higher than that without pretreatment (128.9 mg/L). Due to the potential toxicity of NO2- and NO3- to microorganisms and its bioprocesses, the methane production of sludge pretreated by free nitrous acid was significantly deteriorated. The maximum methane yield (152.0 ± 9.6 mL/g-VSadded) was observed at 10 V pretreatment alone, only 1.7% higher than that of the control (149.4 ± 1.6 mL/g-VSadded). Combined pretreatment indeed enhances the sludge solubilization and hydrolysis, but does not always induce an improved anaerobic digestion efficiency. Semi-continuous experiments were conducted to compare the performances and energy efficiencies of two advanced anaerobic digestions (AAD) of sewage sludge with high-temperature thermal pretreatment (HTTP, 160 ± 1 °C and 0.55 MPa for 30 min) and low-temperature thermal-alkaline pretreatment (LTTAP, 60 ± 1 °C and pH 12.0 ± 0.1 for 30 min), which had similar sludge disintegration degree (9.44-9.48%). At the steady period of a SRT 20 d, the two AAD had similar methane production (150.22 ± 9.55 ml/L/d and 151.02 ± 12.56 ml/L/d) and organic matter removals (22.54 ± 2.84% and 23.15 ± 2.46% for volatile solids-VS). 10058-F4 mw The results of high-throughput sequencing showed that the methanogenic pathways of the two AAD were strictly hydrogenotrophic (AAD with HTTP) and hydrogenotrophic/acetoclastic methanogenesis (AAD with LTTAP), respectively. The energy balance analysis suggested that the AAD with LTTAP was superior to that with HTTP because the former had a higher energy efficiency (1.610) than the latter (1.358). In this study, a novel Aureobasidium pullulans GXL-1 strain without melanin secretion was isolated for efficient polymalic acid (PMA) production. The PMA produced by GXL-1 was characterized, and its molecular mass was determined to be 1.621 kDa by gel permeation chromatography. Liquefied corn starch was shown to replace glucose for PMA production by GXL-1 through simultaneous saccharification and fermentation. The PMA titer obtained from batch fermentation was up to 49.0 ± 1.6 g/L in a 10 L fermentor, and the PMA yield and productivity obtained from repeated-batch fermentation were up to 0.50 g/g and 0.34 g/L·h, respectively. Furthermore, process design and techno-economic analysis were performed at an annual output level of 5000 metric tons by SuperPro Designer. Results showed that the production cost of $2.046/kg and payback period of 6.9 years were achieved by repeated-batch fermentation; this provides an economically feasible strategy for industrial-scale production of PMA. Green microalga, Chlamydomonas sp. TRC-1 (C. TRC-1), isolated from the outlet of effluent treatment plant of textile dyeing mill, was investigated for its competence towards bioremediation. Algal biomass obtained after remediation (ABAR) was implied for bioelectricity and biofuel production. C. TRC-1 could completely decolorize the effluent in 7 days. Significant reduction in pollution-indicating parameters was observed. Chronoamperometric studies were carried out using cyclic voltammetry and electrochemical impedance spectroscopy (EIS). Maximum current density, power and power density of 3.6 A m-2, 4.13 × 10-4 W and 1.83 W m-2, respectively were generated in ABAR. EIS studies showed a decrease in resistance of ABAR, supporting better electron transfer as compared to algal biomass before remediation (ABBR). Its candidature for biofuel production was assessed by estimating the total lipid content. Results revealed enhancement in lipid content from 46.85% (ABBR) to 79.1% (ABAR). Current study advocates versatile potential of isolated C. TRC-1 for bioremediation of wastewater, bioelectricity production and biofuel generation. R2R3-MYB transcription factors are important regulators of the growth and development of plants. Here, CmMYB8 a chrysanthemum gene encoding an R2R3-MYB transcription factor, was isolated and functionally characterized. The gene was transcribed throughout the plant, but most strongly in the stem. When CmMYB8 was over-expressed, a number of genes encoding components of lignin synthesis were down-regulated, and the plants' lignin content was reduced. The composition of the lignin in the transgenic plants was also altered, and its S/G ratio was reduced. A further consequence of the over-expression of CmMYB8 was to lessen the transcript abundance of key genes involved in flavonoid synthesis, resulting in a reduced accumulation of flavonoids. The indication is that the CmMYB8 protein participates in the negative regulation of both lignin and flavonoid synthesis.
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