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During a survey performed in sapota orchards of India, from 2015 to 2018, symptoms of phyllody, little leaf, flat stem and witches' broom were observed in three states Karnataka, Kerala and Tripura. The association of phytoplasmas was confirmed in all the symptomatic sapota samples by using nested PCR specific primers (P1/P7, R16F2n/R16R2 and 3Far/3Rev) with amplification of fragments of ~ 1.25 kb and ~ 1.3 kb. Association of three phytoplasma groups, aster yellows with flat stem from Tripura (Lembucherra), clover proliferation with phyllody symptoms at Karnataka (Bengaluru) and bermuda grass white leaf with flat stem and little leaf from Kerala (Thiruvananthapuram) and Tripura (Cocotilla) were confirmed by 16S rRNA gene sequence comparison analysis. Virtual RFLP analysis of 16S rRNA gene sequences using pDRAW32 further classified the sapota phytoplasma isolates into 16SrI-B, 16SrVI-D and 16SrXIV-A subgroups. This is the first report on identification of three phytoplasma groups in sapota in world.Anagallis foemina L. [syn. Lysimachia foemina (Mill.) U. Manns & Anderb.] is an annual, segetal weed from the family Primulaceae, recognized as a very rare and endangered species in many European countries. The rare occurrence of this species is associated with the specificity of the habitats in which it occurs. Knowledge of genetic diversity within and between rare species populations is a crucial step for investigating the causes of extinction as well as developing effective conservation strategies. The current study undertakes the assessment of the genetic variation and population structure of Anagallis foemina L. specimens collected in south-eastern Poland, Volhynian Polesie and West Volhynian Upland based on inter-simple sequence repeats (ISSR) polymorphism. Twenty ISSR primers amplified 374 DNA fragments, of which 79% were polymorphic. The polymorphic information content values ranged from 0.230 to 0.430 with an average of 0.344. An average genetic similarity calculated based on Dice algorithm between all analysed samples was 0.635 (0.28-1.00). The AMOVA study found a significant difference (Φpt = 0.88, P = 0.001) between Anagallis L. genotypes gathered in Volhynian Polesie (VP) and West Volhynian Upland (VU). Analysis indicated, that 89% of the variation existed among groups and 11% within groups. UPGMA analyses grouped A. foemina samples into 2 clearly separated clusters. The plants of the same geographic origin were grouped together. Principal coordinates analysis (PCoA) as well as STRUCTURE also grouped samples in consistence with the collection site, indicating close genetic affinity of plants from the same location. The observed results are typical for fragmented and isolated populations of rare species. Isolation of a small population leads to a decrease in internal genetic variation and to an increase of variation among them. In that case, the conservation of populations from each regional cluster is important to preserve biodiversity.Soil water capacity (SWC) is a very important factor for the artificial cultivation and production of seedling in Cyclocarya paliurus. To understand SWC requirement for seedling cultivation and to investigate morphological and physiological changes under different SWCs, a 100-day SWC treatment was conducted during artificial cultivation; four treatments were 10-20 wt% SWC (W1), 30-40 wt% SWC (W2), 50-60 wt% SWC (W3), and 70-80 wt% SWC (W4). The result showed that W3 was suitable for seedling cultivation. Compared with W3, growth biomass decreased and water content increased at W1, W2 and W4; K, Ca, and Mg content increased under W1, while Na content increased under W4; SOD, PPO, POD, and CAT activity in leaf significantly increased under W1 and W4, of which SOD activity was the highest, and MDA content reached its maximum under W1. W1 and W4 had negative effects on seedling growth, and seedlings adapt to unfavorable water condition by morphological and physiological responses. Our research would be useful for artificial cultivation and management of Cyclocarya species.Drought stress is an important environmental stress that clearly affect biological systems of plants. There is a possibility that growth regulators are able to protect plants under drought conditions. Ascorbic acid (AsA) plays a particular role on growth of plants and protects cells from oxidative damage caused by environmental stresses. This study emphasized the impacts of AsA on improving the drought tolerance of the pepper plants. Based on a factorial arrangement in a completely randomized design, the experiment had two factors. The first factor was drought irrigation within the field capacity, moderate stress (irrigation within the 60% field capacity) and severe stress (irrigation within the 30% field capacity). The second factor was AsA 0 mM sprayed with distilled water, 0.5 mM and 1 mM. The experiment had three replications. Drought stress inhibited plant growth parameters including fruit number, height, weight, yield, chlorophyll a and b, total chlorophyll, carotenoid contents, it caused improvement in activity of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), proline content, anthocyanins, soluble sugars, malondialdehyde (MDA) and H2O2 in the leaves of sweet pepper. Application of AsA contributes to an increase in antioxidant enzymes activity such as SOD, CAT, POD and proline contents, chlorophyll a and b, total chlorophyll, carotenoids, soluble carbohydrates. However, it reduced the content of anthocyanins, MDA and H2O2. Tofacitinib order Based on this study, it can be suggested that ascorbic acid adjusted antioxidant activity, especially after it has been subjected to drought stress.Soil waterlogging is a common problem in some agricultural areas, including regions under soybean (Glycine max) cultivation. In waterlogged soils, soil O2 depletion occurs due to aerobic microorganisms and plants, affecting the metabolic and physiological processes of plants after suffering anoxia in their root tissue. Another harmful factor in this situation is the exponential increase in the availability of iron (Fe) in the soil, which may result in absorption of excess Fe. The present study sought to evaluate the response mechanisms in soybean leaves 'Agroeste 3680' by physiological and biochemical analyses associating them with the development of pods in non-waterlogged and waterlogged soil, combined with one moderate and two toxic levels of Fe. Gas exchange was strongly affected by soil waterlogging. Excess Fe without soil waterlogging reduced photosynthetic pigments, and potentiated this reduction when associated with soil waterlogging. Starch and ureide accumulation in the first newly expanded trifoliate leaves proved to be response mechanisms induced by soil waterlogging and excess Fe, since plants cultivated under soil non-waterlogged soil at 25 mg dm-3 Fe showed lower contents when compared to stressed plants.
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