Notes

Synchronised ribosome profiling involving a huge selection of microbes in the man microbiome.
Indium tin oxide exposure poses a potential health risk, but the exposure assessment in occupational setting remains incomplete and continues to be a significant challenge. To this end, we investigated the association of work type, airborne indium concentration, respirable fraction of total indium, and cumulative indium exposure index (CEI) with the levels of plasma indium (P-In) and urinary indium (U-In) among 302 indium tin oxide target manufacturing and recycling workers in Taiwan. We observed that recycling-crushing produced the highest concentrations of total indium (area 2084.8 μg/m3; personal 3494.5 μg/m3) and respirable indium (area 533.4 μg/m3; personal 742.0 μg/m3). Powdering produced the highest respirable fraction of total indium (area 58.6%; personal 81.5%), where the workers had the highest levels of P-In (geometric mean 2.0 μg/L) and U-In (1.0 μg/g creatinine). After adjusting for the confounder, the CEIs of powdering (βPR = 0.78; βPR = 0.44), bonding (βPT = 0.61; βPT = 0.37), and processing workers (βPT = 0.43; βPT = 0.28) showed significant associations with P-In and U-In, validating its utility in monitoring the exposure. Also, the respirable fraction of total indium significantly contributed to the increased levels of P-In and U-In among workers. The varying levels of relationship noted between indium exposure and the levels of P-In and U-In among workers with different work types suggested that setting the exposure limits among different work types is warranted.The levels and characteristics of atmospheric metals vary in time and location, can result in various health impacts, which increases the challenge of air quality management. We aimed to investigate PM2.5-bound metals in multiple locations and propose a methodology for comparing metal elements across study regions and prioritizing source contributions through integrated health risk assessments. PM2.5-bound metals were collected in the urban, suburban, rural, and industrial regions of Taiwan between 2016 and 2018. We incorporated the positive matrix factorization (PMF) with health risk assessments (considering estimates of the margin of exposure (MOE) and excess cancer risk (ECR)) to prioritize sources for control. We found that the concentrations of Fe, Zn, V, Cu, and Mn (industry-related metals) were higher at the industrial site (Kaohsiung) and Ba, Cr, Ni, Mo, and Co (traffic-related metals) were higher at the urban site (Taipei). The rural site (Hualian) had good air quality, with low PM2.5 and metal concentrations. Most metal concentrations were higher during the cold season for all study sites, except for the rural. Ambient concentrations of Mn, Cr, and Pb obtained from all study sites presents a higher health risk of concern. In Kaohsiung, south Taiwan, PM2.5-bound metals from the iron ore and steel factory is suggested as the first target for control based on the calculated health risks (MOE 10-6). Overall, we proposed an integrated strategy for initiating the source management prioritization of PM2.5-bound metals, which can aid an effort for policymaking.In the environment, microalgae are exposed to a multitude of stressors simultaneously, inducing physiological adjustments. It is well documented that both phosphorus (P) limitation and trace metals exposure affect microalgal physiology. However, investigations regarding the combination of both P limitation and excess trace metals still deserve attention. In the present study, we evaluated the changes in photosynthetic parameters in the green microalga Ankistrodesmus densus acclimated to different P concentrations prior to exposure to Cd. Our results indicate that different concentrations of P in the medium were responsible for significant changes in some parameters, especially those related to photoprotection mechanisms. Cadmium also altered some of these variables in all P scenarios, and greater damage (i.e., synergism) was observed in the combination P-limited and high Cd, with all the evaluated parameters affected under the adverse scenario. check details Among the parameters analyzed, rapid light curves were the most sensitive to exposure of one or the combination of both stressors (Cd and P limitation). Based on our data, we suggest that P-limited algae activated photoprotective mechanisms as a response to nutrient limitation, especially at the most limited condition. The addition of Cd did not change linearly the parameters related to photoprotection mechanisms under P-limitation, i.e., synergism was observed in the intermediate P-limitation combined with Cd, while in the most P-limited, P seems to be the driving force affecting these mechanisms. Based on our results, we suggest the use of rapid light curves as a tool to complement the assessment of the impacts of stressors, such as metals, in ecotoxicological studies.The public around the world is increasingly concerned about male reproductive health. The impact of nickel nanoparticles (Ni NPs) on male reproductive toxicity including sperm production, motility and fertilizing capacity has been confirmed by our previous researches. In the current study of Ni NPs-inducing toxicity, the expression profiles of piRNAs and their predicted target genes associated with male infertility, were obtained. The results showed that piR-mmu-32362259 was the highest differential expression multiples in both the testis tissues of male mice and GC-1 cells similarly. Notably, piR-mmu-32362259 target gene was significantly enriched in the PI3K-AKT signaling pathway. All these results suggest that piR-mmu-32362259 may affect the occurrence and development of injury in the mouse spermatogenesis process by regulating the PI3K-AKT signaling pathway. In order to verify the result, piR-mmu-32362259 low-expression lentivirus was used to transfect GC-1 cells to establish a stable transfected cell model. The effects of piR-mmu-32362259 on the viability, cycle and apoptosis as well as related protein expression levels of GC-1 cells induced by Ni NPs were detected using CCK8, flow cytometry and western blot assay, respectively. The results showed that low expression of piR-mmu-32362259 could not only alleviate the decrease of GC-1 cell viability, affect the cell cycle and reduce the apoptosis rate, but also significantly affect the expression levels of key proteins and their downstream molecules of PI3K/AKT/mTOR signaling pathway. Collectively, our current results provide a theoretical basis for further exploring the molecular regulatory mechanism of male reproductive toxicity induced by Ni NPs.
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