Notes

Capture-based high-coverage NGS: a powerful instrument to get a large range of mutation sorts.
Proteins are located in the twilight zone between chemistry and biology, where a peculiar kind of complexity starts. Proteins are the smallest 'devices' showing a sensible adaptation to their environment by the production of appropriate behavior when facing a specific stimulus. This fact qualifies (from the 'effector' side) proteins as nanomachines working as catalysts, motors, or switches. However (from the sensor side), the need to single out the 'specific stimulus' out of thermal noise qualifies proteins as information processing devices. Allostery corresponds to the modification of the configuration (in a broad sense) of the protein molecule in response to a specific stimulus in a non-strictly local way, thereby connecting the sensor and effector sides of the nanomachine. This is why the 'disclosing' of allostery phenomenon is at the very heart of protein function; in this chapter, we will demonstrate how a network-based representation of protein structure in terms of nodes (aminoacid residues) and edges (effective contacts between residues) is the natural language for getting rid of allosteric phenomena and, more in general, of protein structure/function relationships.The discovery of hemoglobin allosteric properties is briefly summarized and contextualized in the frame of the main biochemical revelations that characterized the first half of the XX century. In particular, the historical background of DNA, RNA, and protein structure research is recalled and the new role that protein-protein interaction may have on allosteric regulation is discussed.
Quality of life is a fundamental aspect of both clinical practice and research on eating disorders (ED) due to the significant impacts these disorders have on everyday life. Disorder-specific scales can improve the quality of research and findings and offer greater sensitivity and responsiveness. However, no specific instrument is available in Italian for ED. The aim of this paper is to adjust and to validate a reliable scale with specific items regarding physical and interpersonal well-being.

The Italian version of the Eating Disorder Quality of Life (IEDQOL) scale was developed, on the basis of the original English scale, with the addition of items pertaining to physical well-being and interpersonal interactions. In this study, 180 ED patients and 190 healthy controls from the community were enrolled both from inpatient units and outpatient services. A statistical analysis with an exploratory factorial approach was performed in order to validate the tool.

The results showed that the IEDQOL has very good psychometric properties with test-retest validity and sensitivity between patients and controls (d = 2.17 for total score). Moreover, the interpersonal domain showed excellent psychometric values (Cronbach's α > 0.70 in all the subgroups) and a robust correlation with other quality of life constructs.

Future studies on the Italian population should use IEDQOL as outcome element that can be useful also with other disorder-specific psychopathological constructs and corroborate the reliability of the data. Future research in the ED field should only use this specific tool.

Case-control analytic study, Level III.
Case-control analytic study, Level III.High resolution melting (HRM) is a fast closed-tube method for nucleotide variant scanning applicable for bacterial species identification or molecular typing. Recently a novel HRM-based method for Klebsiella pneumoniae typing has been proposed it consists of an HRM protocol designed on the capsular wzi gene and an HRM-based algorithm of strains clustering. In this study, we evaluated the repeatability and reproducibility of this method by performing the HRM typing of a set of K. pneumoniae strains, on three different instruments and by two different operators. The results showed that operators do not affect melting temperatures while different instruments can. Despite this, we found that strain clustering analysis, performed using MeltingPlot separately on the data from the three instruments, remains almost perfectly consistent. The HRM method under study resulted highly repeatable and thus reliable for large studies, even when several operators are involved. Furthermore, the HRM clusters obtained from the three different instruments were highly conserved, suggesting that this method could be applied in multicenter studies, even if different instruments are used.
Radiation exposure during endovascular repair (EVAR) of abdominal aortic aneurysms (AAAs) is a potential issue. Several studies have identified factors affecting radiation exposure, although they are limited. The aim of this study was to identify independent factors affecting radiation exposure in patients with AAA undergoing standard EVAR.

Forty-eight consecutive patients underwent elective EVAR for infrarenal AAA managed between April 2019 and April 2020. Fluoroscopy time (FT) and kerma area product (KAP) were the main outcome measures.

Median (interquartile range) FT and KAP values were 1018 (653-1619) s and 2.68 (2.08-3.81) mGy·m
, respectively. C3 Excluder graft use and main body insertion site from the right femoral were associated with significantly lower FT. Coronary artery disease, endografts with two docking limbs, AAA diameter, neck angle and length, procedure duration, contrast amount, and hospitalization were associated with significantly higher FT. Neck angle was the single independent perioperative factor related to FT higher than the median value observed in the study (P=0.004, odds ratio 1.073, 95% confidence interval 1.023-1.126). The use of the C3 Excluder device was associated with lower KAP. AAA diameter, neck angle, procedure duration, contrast medium amount and postoperative hospitalization were associated with higher KAP. AAA diameter was the single independent factor related to KAP higher than the median value observed in the study (P=0.013, odds ratio 3.73, 95% confidence interval 1.32-10.56).

This study has identified factors affecting radiation exposure during standard EVAR for infrarenal AAAs. These factors should be taken into account when contemplating AAA repair.
This study has identified factors affecting radiation exposure during standard EVAR for infrarenal AAAs. learn more These factors should be taken into account when contemplating AAA repair.
Website: https://www.selleckchem.com/Caspase.html