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Analysis by age stratification did not identify any trends but an increase of a factor of 2 in MCCPs levels was observed over time (p less then 0.05). Plotting the MCCP/SCCP ratio of all available data in humans over time showed also an increasing trend, including for China. The reported levels are relatively low considering the levels reported in environmental media from Australia such which raises the question to what extent CPs accumulate in humans. Future studies on this aspect are required. In this paper, the enhancement of sodium glutamate (SG) on activity of anaerobic ammonia oxidizing (anammox) bacteria was investigated by batch tests. The results illustrated that SG played an important role in enhancing anammox bacterial activity when the SG dosage ranged from 0.50 to 1.25 mM, and the optimal SG concentration was 1.00 mM. The performance of anammox was the best and the total nitrogen removal rate (TNRR) was 138.2 mg N g-1·VSS·d-1 when the concentration of SG was 1.00 mM. The results of EPS and anammox bacterial biomass measurement indicated that protein (PN), polysaccharide (PS), total EPS and the bacterial abundance reached the maximum of 1.00 mM SG addition. Compared to the control tests, the EPS content and bacterial abundance increased by 38.2% and 75.8%, respectively. In addition, the cloning results showed that the community structure of anammox bacteria evolved in species level of Candidatus Brocadia genus under the condition of SG enhancement. Persulfate (PS) is widely used for environmental remediation, but its organic contaminant removal performance strongly depends on its activation. In this study, we demonstrate that pyrite (FeS2) can more effectively activate PS than the commonly used FeSO4 for atrazine degradation. When 3.0 mM of PS and 4.2 mM of iron salts were used, the atrazine degradation efficiency of FeS2/PS was 1.4 times that of FeSO4/PS, while the amount of consumed PS in case of FeS2 was only 53% of that by FeSO4. The better PS activation performance of FeS2 could be attributed to its slow and sustainable release of dissolved Fe(II), inhibiting the quenching reaction between •SO4-/•OH and Fe(II) ions, and thus producing more reactive oxygen species for the atrazine degradation. More importantly, the surface bound Fe(II) of FeS2 could activate molecular oxygen to generate superoxide radical (•O2-), which could further promote the effective decomposition of PS by accelerating the Fe(III)/Fe(II) redox cycle. This study unravels the roles of dissolved Fe(II) and surface bound Fe(II) on the persulfate activation, and provides a promising heterogeneous persulfate activator for pollutant control and environmental remediation. TTK21 concentration Carbon source and dissolved oxygen are the critical factors which sustain the stable redox environment for the microbes to implement the removal of nitrogen and organics in vertical flow constructed wetlands (VFCWs). The effect mechanisms of the COD/N ratios in intermittently aerated VFCWs are needed to be investigated in order to increase the synchronous removal efficiency of pollutants. In this study, the combined effects of COD/N ratios (3, 6, 12) and intermittent aeration in VFCWs on pollutant removal, microbial communities and related function genes were studied. The results showed the increase of COD/N ratios from 3 to 12 enhanced the removal efficiency of TN, NO3--N and COD. The removals of NH4+-N decreased as the COD/N ratio increased. The optimal removals of TN (87.65%), NH4+-N (93.20%), NO3--N (80.80%) and COD (73.93%) were obtained in VFCW2 (COD/N ratios was 6). Illumina Miseq High-throughput sequencing analysis showed that high COD/N ratios increased the richness and diversity of microbial communities. The absolute abundance of nirK, nosZ, nirS, amoA, nxrA, and anammox bacterial 16S rRNA presented various changes under the different ratios of COD/N. The increase of COD/N ratios enhanced the copy numbers of nirS, nirK and nosZ, which participate in denitrification process. High COD/N ratios (6 and 12) were in favor of Actinobacteria, Firmicutes and Chloroflexi, which mainly play important roles in the process of denitrification. This paper implies that the combination of carbon source and aeration is necessary to sustain high microbial activities during pollutant removal in VFCWs. The distribution of Hg, Cd, Cu, and Zn in keratinized tissues, blood fractions, and excretory organs, and MTs in blood fractions and excretory organs was determined in captive, semicaptive, and wild Morelet's crocodiles and they were compared to select the most useful non-destructive tissues for the monitoring of metal exposure and to assess the potential of MTs as a biomarker. Our results indicate blood plasma, claws, and caudal scutes altogether are suitable tissues for xenobiotic metals exposure, with concentrations in blood plasma being an indicator of recent exposure, whereas concentrations in claws and caudal scutes are indicators of chronic exposure. Results in keratinized tissues suggest they are an important detoxification strategy in crocodiles, and claws presented the highest concentrations of metals in both captive (Hg = 0.44 ± 0.23 μg g-1, Cd = 11.10 ± 5.89 μg g-1, Cu = 45.98 ± 23.18 μg g-1, Zn = 124.75 ± 75.84 μg g-1) and wild populations (Hg = 1.31 ± 0.32 μg g-1, Cd = 26.47 ± 21.15 μg g-1, Cu = 191.75 ± 165.91 μg g-1, Zn = 265.81 ± 90.62 μg g-1). Thus, they are an appropriate tool for assessing metal exposure in populations where scutes clipping as a marking technique is not allowed, and their collection is less complicated than with other tissues. MTs are a suitable biomarker in blood plasma, whereas in erythrocytes detoxification processes might depend on hemoglobin, rather than MTs. Future studies should consider the implementation of these tools for the monitoring of wild populations. Manganese is a metal often found as an environmental pollutant and very associated with neurological disorders when in high concentrations. However, little is known about the effects that this contaminant can cause when in environmentally relevant concentrations and occurrence, that is, much lower than those commonly studied. So, the aim of the study was to evaluate the effects that environmentally relevant concentrations of this metal would cause in different zebrafish organs (brain, liver, and blood). Acute 96-h and chronic 30-day exposures were performed using the manganese chloride salt as a pollutant. Behavioral alterations of anxiogenic type were observed in the animals after chronic exposures to 4.0 mg L-1 MnCl2, which traveled a greater distance at the bottom of the aquarium. This may be associated with neuronal damages in the telencephalic region responsible for motor and cognitive activity of the fish, observed in animals from the same exposure. In addition, hepatic histopathological damage as vacuolization of hepatocytes and genotoxic damage, identified by comet assay and micronucleus test, was also observed after acute and chronic exposure, especially at the highest pollutant concentrations (8.
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