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Histone H3 Bosom in Serious COVID-19 ICU People.
The ultrasonic-assisted alkali extraction of Typha domingensis stem polysaccharide (TDSPs) was studied using the response surface methodology. The optimal parameters of TDSPs with maximum yields (12.24± 0.08%) were as follows extraction time 40 min, NaOH concentration 1.5 M and the ratio of water to raw material 25mL/g. The experimental purity of TDSPs was 86.01 ± 0.02. Mineral elements were determined by ICP-AES. The gel permeation chromatography results indicated that TDSPs was a polysaccharide polymer with two peaks with molecular weights of 3182.6 Da (P1) and 3,076,900 Da (P2). The TDSPs consisted of arabinose, rhamnose, galactose, xylose, glucose, mannose, and fructose. The results of NMR and FT-IR spectra represented the presence of β-configurations in TDSPs. Moreover, the TDSPs improved the stimulating effect on the growth of selective probiotic bacteria and showed relatively good antioxidant activity. Therefore, due to its good prebiotic and antioxidant activity, TDSPs could be exploited as a novel natural component in functional food industries.Serotonin (5-HT) receptors have been shown to homodimerize and heterodimerize with other G protein-coupled receptors (GPCRs), although the details of this process have not yet been elucidated. Here we use coarse-grained molecular dynamics on monomeric 5-HT2C receptors to predict the transmembrane (TM) helices involved in such associations. All these simulations were carried out both in 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) lipid bilayers and in mixed composition POPC-Cholesterol ones, to show whether the presence of cholesterol could directly influence and drive the dimeric association. The goal is to get insights on the self-assembly pathway leading to GPCRs 5-HT2C oligomerization, which is supposed to be the basis of its constitutional activity. From the analysis of the molecular dynamics trajectories, we observed the formation of 5-HT2C oligomers through self-assembly and we identified the main domains involved in the receptor dimerization. In particular, dimers and oligomers from the two different environments show TM4-TM5 and TM1-TM7-H8 as the preferential dimerization interfaces. Nevertheless, substantial differences arise for oligomers in POPC and in POPC-Chol membranes in POPC-Chol the variability of dimers interfaces is strictly limited to the TM1-TM7-H8 and TM4-TM5 interfaces and the dimorphism depends on cholesterol that directly participates in its formation. These results are in agreement with both experimental evidences and other computational studies conducted on other GPCRs oligomerization.Liver injury is a common pathological process, which can result in fatty liver, cirrhosis, fibrosis and even cancer. Polysaccharides isolated from plants have been regarded as an important resource of anti-hepatic lesion due to widely distributed in nature and low toxicity. In order to have a better understand of the protective mechanism on liver function, a comprehensive review of research into plant polysaccharides during recent five years was performed. In total, 66 types of polysaccharides from 58 kinds of plant have shown hepatoprotective effect through the pathological process of inflammation, apoptosis and oxidative stress by regulating NF-κB, JAK/STAT, TGF-β, PI3K/AKT, MAPK, caspase cascade, p53 and Nrf2-Keap1 pathways, lipid metabolism as well as cytochrome P450 enzymes. Moreover, correlations between structure and hepatoprotective activities of plant polysaccharides including primary structure, conformation properties, structural modification and content of uronic acid were also preliminarily explored. This review will provide a comprehensive perspective for better understanding the mechanism and development of polysaccharides against liver injury.The aim of this study was to evaluate the influence plasticizer (glycerol (GLY)/sorbitol (SOR)) and antimicrobial (potassium sorbate (KS)/grapefruit seed extract (GFSE)) agents on crystallinity, water barrier, mechanical, thermal and anti-fungal properties of prepared with corn starch (CS)-chitosan (CH) nanoclay (Na-MMT) bionanocomposite films. Results showed that CS/CH/nanoclay/SOR/GFSE films exhibit a higher crystallinity than any other bionanocomposite films. Molecular miscibility among corn starch, chitosan (COOH, NH) and nanoclay (AlOH, SiOSi) was exhibited in XRD. Films plasticized with SOR showed the highest tensile strength, lowest film solubility, lowest water vapor permeability and thermal stability. Fourier transform infrared spectroscopy (FTIR) confirmed that the main interactions among the components in a bionanocomposite film are due to hydrogen bonding. this website Bionanocomposite films containing GFSE showed a maximum zone of inhibition against Aspergillus niger. Synthetic plastic films exhibited fungal growth on 6th day whereas CS/CH/nanoclay/SOR/GFSE films did not show the same up to 20 days when bread samples were packed at 25 °C and 59% RH. CS/CH/nanoclay/SOR/GFSE film could potentially be useful for active packaging in extend the shelf life; maintain its quality and safety of food products thus substituting synthetic plastic packaging materials.Physicochemical properties of microbial transglutaminase (MTGase) cross-linked surimi gels subjected to liquid nitrogen (LN) spray freezing with different temperatures and cross-linking degrees were investigated. Gels with lower LN spray temperature (-90 °C) were found taking less time in cooling down themselves to maximum-ice-crystal generating temperature. Microstructure images showed the pores of gels became smaller and the structure gradually became denser, as freezing temperature decreased and cross-linking degree increased. It also revealed T22 relaxation time of gels decreased significantly with surimi gels cross-linking degree increasing, indicating the binding ability of gels to moisture was enhanced accordingly. Meanwhile, the proton density weighted image brightness declined along with the LN spray temperature decreasing, and the image brightness showed a decreasing trend from outside to inside, indicating that water migrated and permeated easier from the inside of the gel network to the outside undergone higher LN temperature and lower cross-linking degree.
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