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Considering that hyperaccumulators can accumulate high concentrations of iron salt, they can successfully obtain magnetic hydrochar from iron-rich hyperaccumulators. click here In this study, iron-rich biomass was obtained by irrigating Phytolacca acinosa Roxb. using iron salt. Magnetic nano-Fe3O4 hydrochar was prepared from iron-rich Phytolacca acinosa Roxb. via hydrothermal carbonization to remove Cd. The characterization results showed that the synthesized magnetic nanoparticles had an average size of 2.62 ± 0.56 nm and N elements were doped into magnetic nano-Fe3O4 hydrochar with abundant oxygenic groups. Cd adsorption on magnetic nano-Fe3O4 hydrochar was better fitted using the Langmuir isotherm and the pseudo-second-order kinetic model. The maximum adsorption capacity was 246.6 mg g-1 of Cd. The research confirmed that Cd adsorption was controlled by multiple mechanisms from the jar test, transmission electron microscopy mapping, scanning electron microscopy-energy dispersive X-ray spectroscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy. CdCO3 crystals can be formed after adsorption, indicating that surface precipitation played an important role in Cd adsorption. The abundance of O atoms and the doping of N atoms on the hydrochar surface were conducive to Cd adsorption, indicating that the mechanisms were related to surface complexation and electrostatic attraction. In addition, the significant decrease in Na+ content after Cd adsorption illustrated that ion exchange had a non-negligible effect on Cd adsorption. This study not only provides a strategy for preparing magnetic nano-Fe3O4 hydrochar derived from iron-rich plants but also verifies multiple Cd adsorption mechanisms using magnetic nano-Fe3O4 hydrochar.Cable bacteria are filamentous sulfur-oxidizing microorganisms that couple the reduction of oxygen or nitrate in surface sediments with the oxidation of free sulfide in deeper sediments by transferring electrons across centimeter scale distances. The distribution and activities of cable bacteria in freshwater sediments are still poorly understood, especially the impact of cable bacteria on sulfur cycling. The goal of this study was to investigate electrogenic sulfide oxidation associated with cable bacteria in laboratory microcosm incubations of freshwater sediments using microsensor technology, 16S full-length rRNA sequencing, and fluorescence in situ hybridization (FISH) microscopy. Their activity was characterized by a pH maximum of 8.56 in the oxic zone and the formation of a 13.7 ± 0.6 mm wide suboxic zone after 25 days of incubation. Full-length 16S rRNA gene sequences related to cable bacteria were recovered from the sediments and exhibited 93.3%-99.4% nucleotide (nt) similarities with those from other reported freshwater cable bacteria, indicating that new species of cable bacteria were present in the sediments. FISH analysis indicated that cable bacteria density increased with time, reaching a maximum of 95.48 m cm-2 on day 50. The cells grew downwards to 40 mm but were mainly concentrated on the top 0-20 mm of sediment. The cable bacteria continuously consumed H2S in deeper layers and oxidized sulfide into sulfate in the 0-20 mm surface layers, thereby affecting the sulfur cycling within sediments. These findings provide new evidence for the existence of higher diversity of cable bacteria in freshwater sediments than previously known.The tidal creek is an important part of the intertidal zone, which maintains the balance between depositional processes and a given hydrodynamic environment. Much can be inferred about the development and evolution of a tidal creek by examining its morphometry characteristics; this information can also provide scientific decision support for the development and utilization of coastal tidal flats. In this study, we propose a complete system of large-scale tidal creek morphometry characteristic extraction algorithms. This system improved the intelligence of the node classification and the accuracy of the grading, as well as mitigates the interference of island-shaped tidal creeks in the automatic iterative classification process. And this system solves the problem of low post-processing efficiency due to the existence of a large number of discontinuous tidal creek arcs, and greatly reduces the error in the calculation of morphology characteristics. After accuracy verification, using this algorithm, the classifirders showed an increasing trend, but the increasing rate slowed down, from large-scale bifurcation to local etching. Our algorithm represents a significant step forward in the high-precision quantitative detection of tidal creek morphology characteristics, and our results provide evaluable insight into the necessity of monitoring the status and evolution of tidal flats.The effective control and management of nitrate (NO3-) pollution requires the identification of the sources of NO3- pollution in groundwater and quantification of their contribution rates. In this study, the molar concentration ratio of NO3-/Cl- (n(NO3-)/n(Cl-)) and the molar concentration of Cl- (n(Cl-)) (reference ion method; RIM) was first used to identify the NO3- sources and estimate their contribution rates in groundwater. The relationship between the Cl- concentration and NO3- concentration (reference ion method; RIM) was used to judge whether denitrification had occurred and to estimate the denitrification rate in groundwater. It was proved that homology analysis was the prerequisite for applying the RIM. The main NO3- sources included chemical fertilizers (CF), sewage/manure (M&S) and soil nitrogen (SN). The contribution rate of CF in the vegetable planting area (upstream regions) (69.12%) was significantly higher than that in the grain planting area (midstream regions and downstream regions) (14.29% and 14.29%). The difference in the contribution rates of NO3- in the grain planting area was greater than that in the vegetable planting area. The results indicated that denitrification rate in the grain planting area was higher than that in the vegetable planting area, while the temporal variations in the denitrification rate in the vegetable planting area were consistent with in the grain planting area. The RIM offers a useful and simple way to quantify the contribution rates of NO3- sources and denitrification rates in groundwater.
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