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010), involvement of the central nervous system (P=0.050), a high white blood cell count (P=0.020), and tumor lysis syndrome (P=0.010) were the significant prognostic factors for the recurrence and mortality of ALL. Accordingly, careful monitoring in the administration of treatment protocols is suggested to reduce the risk of recurrence and death in these patients.
(
) is the most common cause of urinary tract infection (UTI) and typically treated with antibiotics. Unrestricted use of antibiotics may lead to the emergence of antibiotic-resistant bacteria. The present study aimed to isolate and characterize phages against
from infected urine samples and to determine the lytic activity of phages against
.
The present experimental study was conducted in the Laboratory of Bouali Sina Hospital (Sari, Iran) in May 2018.
was identified from nine urine samples of patients with UTI using conventional microbiological methods. Bacteriophages were isolated from the infected urine specimens, and their lytic activity was determined using the spot test. The titer of the bacteriophages was measured using the double-layer agar technique. The morphology of the bacteriophages was revealed using transmission electron microscopy, and the latent time period and burst size were determined. Data were analyzed using the SPSS software package.
was isolated from nine infected urine samples. The lytic activity of bacteriophages against
was determined using the spot test by observing the formation of inhibition zones. Transmission electron microscopy showed
phages belonging to the Myoviridae family. The latent time period was 20 minutes with a burst size of 1,200 plaque-forming unit (PFU) per infected cell. The results of the double-layer agar assay showed that the titer of bacteriophages was 20×10
PFU/mL.
The
bacteriophage was isolated from infected urine samples and characterized, and their lytic activity against
was determined
.
The E. coli bacteriophage was isolated from infected urine samples and characterized, and their lytic activity against E. coli was determined in vitro.
Leishmaniasis is the most important parasitic disease in Iran and is the third highest rate of rural cutaneous leishmaniasis in the world. Chitosan-polyethylene oxide nanocomposite fibers can be a suitable replacement for ordinary bandages. For the first time, in the absence of any published reports, the present
study aimed to evaluate the anti-leishmanial effects of chitosan (CS)-polyethylene oxide (PEO)-berberine nanofibers on
.
The present experimental study was conducted in 2018 in Tehran, Iran. The CS-PEO nanofibers containing berberine, as a natural anti-parasitic agent, were prepared using the electrospinning technique. Biocompatibility and fibroblast proliferation on nanofibers were investigated. In addition, the anti-leishmanial activity of CS-PEO nanofibers in both the promastigote and amastigote stages of
was evaluated after parasite vital staining and MTT assay and compared to a control group. Statistical analysis was performed using SPSS software (version 18.0). Statistically significd amastigote stages in vitro. Further studies are required to investigate the effects of this nanofiber on leishmanial ulcers in laboratory animals and clinical cases.
Microbial plaque-induced oral diseases are among the most common diseases worldwide. The present study aimed to compare the antimicrobial effect of electrolyzed water (EW), (acidic, mildly basic, and basic) on the growth of bacterial species producing dental plaque and to assess their cytotoxicity on fibroblasts and epithelial cells.
The study was performed at Shahid Beheshti University of Medical Sciences in 2019. Several bacterial species (
and
) were treated with different EW types at three pH values (3, 9, and 11) for 30 seconds and subsequently, the colonies were counted. The cytotoxic effect of these EW types was evaluated on HeLa and L929 cell lines at 30 seconds, 1 minute, and 5 minutes. GraphPad Prism 6.0 was used for statistical analysis. The Kruskal-Wallis test followed by Mann-Whitney U and one-way analysis of variance followed by Tukey's test were used to analyze bacterial activity and cell cytotoxicity, respectively. P<0.05 was considered statistically significant.
EW types significEW could vary depending on cell types and treatment periods.
Mesenchymal stem cells (MSCs) are widely used to treat various diseases, however, their proliferative potential reduces after a number of passages. It has been shown that some probiotics such as
(
) affect the proliferation of various cell lineages. The present study aimed to investigate the effect of
on the proliferation of rat bone marrow stromal cells (rBMSCs) and to develop a method for compensating their proliferation reduction after some passages.
The present experimental study was conducted at Tehran University of Medical Sciences, Tehran, Iran, in 2017. The stromal cells were isolated from rBMSCs and their mesenchymal properties were confirmed by osteogenic and adipogenic differentiation media and staining.
was cultured and the
supernatant (BS) and bacterial cell mass (BCM) were extracted. The rBMSCs were treated with different concentrations of BS and BCM. The MTT assay was performed to measure the number of viable cells in the culture. Cell proliferation was analyzed using the paired-sample t test.
Cell proliferation increased as the concentration of bacteria was increased logarithmically (0, 0.1, 0.3, 0.9, 3, 9, 30 μL/mL). In comparison with BS, cells treated with BCM showed increased cell proliferation at lower concentrations. This effect was caused by removing the "de Man, Rogosa, and Sharpe" (MRS) broth medium from the BCM culture. The optimal concentration of bacteria with the most significant effect on rBMSCs proliferation was determined.
A significant increase in the proliferation of stromal cells was observed; confirming the stimulatory potential of probiotics (
) on various cells. The use of products containing probiotic bacteria can increase the proliferation potential of BMSCs.
A significant increase in the proliferation of stromal cells was observed; confirming the stimulatory potential of probiotics (B. Oxaliplatin research buy bifidum) on various cells. The use of products containing probiotic bacteria can increase the proliferation potential of BMSCs.
Website: https://www.selleckchem.com/products/Eloxatin.html
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