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Antiplatelet Activity involving Coumarins: In Vitro Assays on COX-1.
Vascular calcification (VC) is a vital factor for cardiovascular morbidity and mortality. Accumulating data suggest that microRNA (miR) is implicated in the VC. The main purpose of this study is to study the influence of miR-155-5p overexpression on VC development in vitro and in vivo. Immunofluorescence staining, real-time PCR, alizarin red staining, alkaline phosphatase (ALP) activity assay, western blot, luciferase assay, hematoxylin-eosin (HE), Masson's trichrome staining, and calcium content assay were used in this research. The results showed that miR-155-5p was decreased in the rat vascular smooth muscle cells (rVSMCs) undergoing calcification in vitro. MiR-155-5p overexpression reversed the increase of calcification and ALP activity in calcified cells. Further, overexpression of miR-155-5p inhibited the transforming growth factor-β1 (TGF-β1)/Smad2/3 signaling pathway, as evidenced by decreased protein expression of TGF-β1, pSmad-2 and pSmad-3 in rVSMCs. MiR-155-5p was showed to target Smad2 directly. Moreover, miR-155-5p upregulation reduced vascular thickening, fibrosis and calcium content of aorta abdominalis in CaCl2-mediated VC model. Collectively, our results suggest that miR-155-5p overexpression may inhibit VC development through suppressing TGF-β1/Smad2/3 signaling pathway in vivo and in vitro, indicating that miR-155-5p may act as a potential therapeutic target for VC-related disease. INTRODUCTION The Speech, Spatial and Qualities of Hearing Scale has been widely used to assess the subjective sense of auditory ability, functional hearing loss and the resulting benefit of the hearing correction strategy. OBJECTIVE To translate and culturally adapt the Speech, Spatial and Qualities of Hearing Scale to the Colombian Spanish from Brazilian Portuguese by means of a final version that demonstrates an understanding percentage greater than 85%. METHODS The study was divided into three phases in the first one the translation was done, the retro translation and the modifications were defined by the evaluation team, and in the other two, two pilot tests were made to 50 participants in the first one the understanding of each of the statements that made up the Speech, Spatial and Qualities of Hearing Scale was examined in 25 people and adjustments were made, and in the second, the same procedure was carried out in 25 other individuals but the document was not changed. RESULTS It was observed that during the pilot test 1, there was difficulty in questions number 2 of Part 1 (56% understanding), and in 8, 9, 10, 12, 16 and 17 of Part 3 (75%) of understanding), while in others, the degree of understanding was higher than 85%. However, in pilot test 2, understanding was above 85% in all questions. In addition, Cronbach's alpha (0.93) indicated that the items from which the test was constituted measured the same construct and were reliable. CONCLUSION The method used allowed obtaining the version of the Speech, Spatial and Qualities of Hearing Scale in Colombian Spanish with an understanding percentage greater than 85%. INTRODUCTION Functions attributed to androgens have increased, ranging from the role in hypothalamic-pituitary-gonadal axis and reproductive behaviors to modulation of cognition, mood and some other functions. Sex differences and changes in circulating sex hormones affect human sensory function. In the literature, authors reported this kind of influence for olfaction predominantly in females. OBJECTIVE To investigate the effects of low testosterone levels on olfactory functions in males, in this prospective clinical study. METHODS Male patients diagnosed with prostate cancer were included. Thirty-nine patients with prostate cancer whose testosterone levels were lower than 50ng/dL due to castration, were the study group. Thirty-one patients with prostate cancer who were not castrated with testosterone levels higher than 50ng/dL were selected as the control group. Acoustic rhinometry and peak nasal inspiratory flow tests were performed for all participants; and for evaluation of olfactory function, both groups completed the Connecticut chemosensory clinical research center olfactory test. RESULTS The mean ages of the patients and controls were 69.6±7.2 (57-89) and 66.3±5.8 (50-78) years, respectively (p=0.039). There was a significant difference between groups in terms of testosterone levels (p less then 0.0001). The multivariate logistic regression revealed testosterone level as the only predictive factor determining the difference between the groups. In terms of olfactory parameters, all scores were lower in the emasculated group (butanol threshold test p=0.019, identification p=0.059, and Connecticut center score p=0.029) There was a significant correlation between testosterone levels and olfactory parameters (p=0.023; p=0.025 for identification and Connecticut center scores, respectively). CONCLUSION Low testosterone levels in males have negative effects on olfactory functions. Further molecular research is required to understand the connection between testosterone and olfaction. C-X-C motif chemokine 17 (CXCL-17) is a novel chemokine that plays a functional role maintaining homeostasis at distinct mucosal barriers, including regulation of myeloid-cell recruitment, angiogenesis, and control of microorganisms. Particularly, CXCL17 is produced along the epithelium of the airways both at steady state and under inflammatory conditions. check details While increased CXCL17 expression is associated with disease progression in pulmonary fibrosis, asthma, and lung/hepatic cancer, it is thought to play a protective role in pancreatic cancer, autoimmune encephalomyelitis and viral infections. Thus, there is emerging evidence pointing to both a harmful and protective role for CXCL17 in human health and disease, with therapeutic potential for translational applications. In this review, we provide an overview of the discovery, characteristics and functions of CXCL17 emphasizing its clinical potential in respiratory disorders. OBJECTIVE The ongoing pandemic coronavirus disease-2019 (COVID-19) infection causes severe respiratory dysfunction and has become an emergent issue for worldwide healthcare. Since COVID-19 spreads through contact and droplet infection routes, careful attention to infection control and surgical management is important to prevent cross-contamination of patients and medical staff. Tracheostomy is an effective method to treat severe respiratory dysfunction with prolonged respiratory management and should be performed as a high-risk procedure METHOD The anesthetic and surgical considerations in this case involved difficult goals of the patient safety and the management of infection among health care workers. Our surgical procedure was developed based on the previous experiences of severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV). RESULTS We described the management procedures for tracheostomy in a patient with COVID-19, including the anesthesia preparation, surgical procedures, required medical supplies (a N95 mask or powered air purifying respirator, goggles, face shield, cap, double gloves, and a water-resistant disposable gown), and appropriate consultation with an infection prevention team.
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