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Changing Compositional Properties of Well-liked Genomes to create Live-Attenuated Vaccines.
© 2020 Published by Elsevier Ltd.Perlite is a natural glassy volcanic rock used in construction applications requiring improved lightweight, thermal, and acoustic properties. During processing of raw perlite (i.e., cutting and fractioning to different sizes), large amounts of powders are collected and stored as waste materials. This paper evaluates the effect of waste perlite (WP) powders on durability and long-term transport properties of self-consolidating concrete (SCC). Different mixtures prepared with 580 kg/m3 powder using various combinations of WP, limestone filler (LF), metakaolin (MK), and silica fume (SF) are tested over 2-years period. Test results showed that WP confers particular benefits to the SCC compressive strength and its evolution over time, particularly when used in combination with MK and SF. Water permeability, carbonation, and chloride ion migration curtailed when WP concentration reached 220 and 260 kg/m3. In contrast, the resistance against freeze/thaw remarkably improved, given the pozzolanic reactions and porous nature of such powders that accommodated the disruptive expansive stresses resulting from frost attack. © 2020 The Authors. Published by Elsevier Ltd.Oil mill wastewater (OMW) is the main liquid discharge from oil mills, it is considered as a dangerous pollutant due to its toxic chemical compounds which are unloaded directly in the environment without any treatment. The aims of this study were to evaluate the effectiveness of OMW adsorption on clay as a good method for the elimination of toxic chemical compounds and to study the application of treated OMW as an irrigation source in agricultural field. For this, Clay was collected from the city of Agourai (Meknes region, Morocco) and characterized by X-ray diffraction, X-ray fluorescence spectrometry, BET and FTIR analysis. Moreover, the treated OMW was analyzed using UHPLC-ESI-MS and the determination of total phenolic content (TPC) was also performed. However, the application of the treated OMW in agricultural field was performed by the determination of its effect on the germination of Lepidium sativum seeds (in vitro) and as a source of irrigation of Vicia faba plants (in situ). The results of this study showed that OMW had the following physicochemical characteristics average pH of 4.88, TPC of 4.75 g/l, COD of 80 g/l, BOD5 of 18.72 g/l, conductivity of 16.05 cm-1, dry matter of 135.7 g/l and volatile matter of 58.7 g/l. The adsorption on clay had increased the pH from 4.88 to 6.14 and reduced significantly the organic matter (42% of COD and 57.4% of phenolic compounds). UHPLC-ESI-MS analysis showed the presence of a wide variety of organic compounds in OMW, with the appearance of new compounds after adsorption. Moreover, the use of treated OMW as a source of irrigation showed a significant effect on the germination of Lepidium sativum seeds and the growth of Vicia faba plants. From this study, we can conclude that the adsorption on clay is a good method for the treatment of OMW, which became non-toxic for environment and can be used as a source of irrigation in agricultural field. © 2020 Published by Elsevier Ltd.Measurement of the antioxidant potential using in vitro assays is paramount in the assessment of various food products and nutraceuticals. Researchers always attempt to develop more accurate assays which can be performed in unsophisticated conditions. This novel method, Ferric-Bipyridine reducing capacity of total antioxidants (FBRC) is a very simple, accurate assay performed based on the reduction of Fe (III) to Fe (II) by antioxidants with the formation of a colored complex with bipyridine (Bp) i.e, Fe(II)-Bp. The FBRC method thus developed was assessed under carefully adjusted parameters of oxidant concentration, pH, temperature, solvent, light and time in order to fix the optimum conditions for the assay. The spectrophotometric monitoring of Fe(II)-Bp complex was noted by the formation of an intense pink color at room temperature with absorption maxima at 535 nm, pH 4. The analytical performance of this method was fully validated, and the obtained results were satisfactory. It was successfully applied to measure the total antioxidant capacity of standard compounds such as gallic acid, ascorbic acid and butylated hydroxy toluene (BHT), in addition to some plant extracts and oils. The FBRC method is inexpensive, reproducible and simple to perform. In addition, the antioxidant activity of the tested compounds compared to common reference methods showed that the novel FBRC method is superior to the Ferric reducing antioxidant power (FRAP) with regard to its use of realistic pH and faster kinetics. Thus, the FBRC method is convenient for the estimation of total antioxidant in plants extracts, natural products, essential oils and food stuff. © 2020 The Authors.In-silico activity prediction was performed to predict new inhibitory activities of 2, 9-disubstituted 8-phenylthio/phenylsulfinyl-9h-purine derivatives as anti-proliferative agents using QSAR technique. The anti-proliferative agents were optimized using Density Functional Theory (DFT) method utilizing the B3LYP/6-31G* level of theory. Genetic Function Algorithm (GFA) was used to build the QSAR models. Out of the models built, the best one was selected and reported because of its fitness statistically with the following assessment parameters R2 trng = 0.919035, R2 adj = 0.893733, Q2 cv = 0.866475, R2 test = 0.636217, and LOF = 0.215884. DL-AP5 price The selected model was further subjected to other assessment such as VIF, Y-scrambling test, applicability domain and found to be statistically significant. The binding mode of some selected 2, 9-disubstituted 8-phenylthio/phenylsulfinyl-9H-purine (ligands) in the active site of EGFR-tyrosine kinase (EGFR-TK) (receptor) was studied via Molecular docking. Molecule 22 was identified to have the highest binding energy (-10.4 kcal/mol) among the other selected ligands which it might be as a result of hydrogen interactions formed with MET793 (2.48599 Å, 2.04522 Å) & THR854 (3.76616 Å) amino acid residues and hydrophobic/other interactions with amino acid residues (LEU718, LEU844, MET766, VAL726, ALA743, LYS745 and MET790) in the active site of EGFR-tyrosine kinase (EGFR-TK). The drug-likeness of these selected anti-proliferative agents were predicted via the pharmacokinetics profile of the molecules utilizing SWISS ADME. The anti-proliferative agents were found to be orally safe by not having more than 1 violation of the Lipinski's rule of five. This research proposed a way for designing potent anti-proliferative agents against their target enzyme. © 2020 The Authors. Published by Elsevier Ltd.
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