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Complexity in water distribution systems (WDSs) poses a challenge for analysis and management of the systems. To reduce the complexity, the recent development of complex network science provides a system decomposition technique that converts a complex WDS with a large number of components into a simple system with a set of interconnected modules. selleck kinase inhibitor Each module is a subsystem with stronger internal connections than external connections. Thus far, the topological features of the modular structure in WDS have been extensively studied but not the behavioural features, e.g. the hydraulic interdependencies among modules. Therefore, this paper aims to quantitatively measure and graphically visualize the module interdependency in WDSs, which helps understanding the behavioural complexity of WDSs and thus various WDS analyses, such as pipe maintenance, model calibration, rehabilitation, and District Metered Areas planning. Specifically, this study first identifies the WDS's modular structure then measures how changes in the state of one module (i.e. any single pipe failure or perturbed demand within each module) affect the state of another module. Modular interdependencies are summarized in an interdependency matrix and visualized by the digraph. Four real-world systems are analysed, and three of them shows low interdependencies among most of the modules and there are only a few critical modules whose status changes will substantially affect a number of other modules. Hence, highly interconnected topologies may not result in strong and complex module interdependency, which is a fact that simplifies several WDS analysis for practical applications as discussed in this paper.Nearly half a billion people living in Indian cities receive their drinking water from an intermittent water supply (IWS), which can be associated with degraded water quality and risk of waterborne disease. The municipal water supply in Nagpur, India is transitioning from intermittent to continuous supply in phases. We conducted cross-sectional sampling to compare microbial water quality under IWS and continuous water supply (CWS) in Nagpur. In 2015 and 2017, we collected 146 grab samples and 90 large-volume dead-end ultrafiltration (DEUF) samples (total volume 6,925 liters). In addition to measuring traditional water quality parameters, we also assayed DEUF samples by droplet digital PCR (ddPCR) for waterborne pathogen gene targets. At household taps served by IWS, we detected targets from enterotoxigenic E. coli, Shigella spp./enteroinvasive E. coli, norovirus GI and GII, adenovirus A-F, Cryptosporidium spp., and Giardia duodenalis. We observed a significant increase in the proportion of grab samples positive for culturable E. coli (p = 0.0007) and DEUF concentrates positive for waterborne pathogen gene targets (p = 0.0098) at household taps served by IWS compared to those served by CWS. IWS continues to be associated with fecal contamination, and, in this study, with increased prevalence of molecular evidence of waterborne pathogens. These findings add mounting evidence that, despite the presence of piped on premise infrastructure, IWS is less likely to meet the requirements for safely-managed drinking water as defined by the Sustainable Development Goals. Importantly, these findings demonstrate the transition from IWS to CWS in Nagpur is yielding meaningful improvements in microbial water quality.Natural Deep Eutectic Solvents (NADES) are composed of supramolecular interactions of two or more natural compounds, such as organic acids, sugars, and amino acids, and they are being used as a new media alternative to conventional solvents. In this study, a new application of NADES is presented as a possible technology for biofilm structural breaker in complex systems since the current solvents used for biofilm cleaning and extraction of biofilm components use hazardous solutions. The NADES (betaineureawater and lactic acidglucosewater) were analyzed before and after the biofilm treatment by attenuated total reflection Fourier-transform infrared spectroscopy and fluorescence excitation-emission matrix spectroscopy. Our results indicate that the green solvents could solubilize up to ≈70 percent of the main components of the biofilms extracellular matrix. The solubilization of the biomolecules weakened the biofilm structure, which could enhance the biofilm solubilization and removal. The NADES have the potential to be an environment-friendly, green solvent to extract valuable compounds and break the main structure from the biofilm, leading to a greener method for extracellular polymeric substance (EPS) extraction and biofilm treatment in various water treatment systems.Recent studies have found that blueberry supplementation can improve parameters related to metabolic syndrome (MetS); however, there is no definitive consensus. Analysis of several randomized controlled trials can demonstrate whether a reduced effect of MetS risk factors is more pronounced in individuals who received supplementation with blueberry than in individuals who did not receive this supplementation. This systematic review and meta-analysis aimed to investigate the effect of blueberry intervention on MetS risk factors, including blood pressure, anthropometric measurements, and glycemic and lipid profiles. PubMed, Scopus, Web of Science, and SciELO were systematically searched to identify relevant studies published before July 2020. To compare the effects of blueberry supplements (powder, extract, fruit, juice, or frozen) with placebo, the mean differences with 95 % confidence intervals (CI) were pooled based on the random-effects model. We classified the quality of evidence according to the GRADE approach. In total, 18 randomized controlled trials (RCTs) were included in this systematic review, and 12 studies were selected for meta-analysis. Based on the Cochrane Collaboration risk-of-bias tool, all studies were of good quality. These trials differed with regards to blueberry dosage and forms, recruited subjects, and trial duration. Meta-analyses of the data showed that blueberry intervention had a significant effect on lipid levels, decreasing total cholesterol and low-density lipoprotein (LDL) levels. We found no significant differences in the glycemic status markers and anthropometric measurements. Blueberry supplementation significantly decreased diastolic blood pressure. In conclusion, the meta-analysis showed that blueberry may be efficacious in the treatment of MetS, due to its beneficial effects on lipid and blood pressure markers.
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