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A cell culture opposite transcriptase quantitative PCR (ICC-RTqPCR) strategy to simultaneously measure the actual contagious concentrations regarding eight environmentally related enterovirus serotypes.
A high-energy, high-beam-quality, high-contrast picosecond optical parametric chirped-pulse amplification (ps-OPCPA) laser system was demonstrated. The pulse from a femtosecond oscillator was stretched to 4 ps, after which it was amplified from 140 pJ to 600 µJ by an 8 ps/6 mJ pump laser in two non-collinear OPCPA stages. The total gain was >106, and the root mean square of the energy stability of the laser system was 1.6% in 10 h. Tacrolimus The contrasts of the solid and fiber mode-locked femtosecond oscillator-seeded ps-OPCPA systems were compared, and a signal-to-noise ratio of >1011 was achieved. Using this system, the contrast of the front end in high-power picosecond petawatt laser facility was improved by ∼40 dB to >1011, beyond ∼200 ps ahead of the main pulse with an output level of 60 mJ.To accommodate the demand of exponentially increasing global wireless traffic driven by the coming beyond 5G and 6G, wireless communication has stepped into the millimeter wave (MMW) band to exploit large available bandwidth. The future wireless application scenarios require wireless communication systems with high speed, low cost, a small footprint and simple configuration, and the integrated light source-based intensity modulation and direct detection (IM-DD) photonic-wireless system can better meet the demand than the traditional system based on bulky components. In this paper, we experimentally demonstrate a lens-free pulse-amplitude-modulation with four levels (PAM-4) and discrete multi-tone with 16-quadrature amplitude modulation (DMT-16QAM) MMW photonic-wireless transmission system in the W-band using an integrated mode-locked laser (MLL) chip and a mixer-based receiver, which could be applicable for flexible wireless applications. The integrated MLL as an on-chip single light source is used to generate W-band signals and simplify the transmitter. The signal-to-noise ratio of the generated wireless signal is improved by two coherent optical carriers both modulated with data and then beating in the photodiode. In addition, we investigate the IM-DD configuration by employing an envelope detector (ED) to receive the PAM-4 signal for further simplifying the system. The ED-based photonic-wireless system is more suitable for the applications with lower data rate and low cost. For higher data rate, the mixer-based PAM-4/DMT-16QAM systems with up to 31.75 Gbit/s net data rate are more favorable, although the cost is also higher.An exceptionally simple and versatile advance in super-resolution microscopy has been created by adding a new birefringent FINCH holographic lens system including an inexpensive uncooled CMOS camera to a standard microscope. Resolution, after only a single image capture, is equivalent to or better than other more complex popular methods such as SIM, Airyscan and a number of image scanning microscopy methods that boost resolution about two-fold. This new FINCH implementation uniquely works for any objective power and NA and is solid state, fast, and calibration-free. In addition to being as easy to operate and maintain as a standard fluorescence microscope, it can uniquely create super-resolved images with any type or wavelength of light including fluorescence, bioluminescence or reflected light because its principle depends only on emitted light from objects and requires no prior training or knowledge about the sample being imaged. This microscope technique increases the utility and availability of super-resolution microscopy for any user in any research lab.A cyclic atomic level scheme interacting with an optical and a microwave field is proposed for the generation and group-delay control of few-photon optical pulses. Our analysis exploits a hybrid second order-nonlinearity under conditions of electromagnetically induced transparency to generate an optical pulse. The generated pulse can be delayed or advanced through microwave intensity control of the absolute phase of the second-order-nonlinearity. Importantly, this handle on group delay of the generated pulse is number density-independent. Our scheme is thus ideally suited for the generation and control of few-photon optical pulses using ultra-dilute atomic samples. Our results will enable microscopic atomic interface systems that serve as controllable delay channels for both classical and quantum signal processing.Triangular frequency-modulated continuous-wave (FMCW) laser radars (ladars) are extremely sensitive to vibration errors. An FMCW ladar 3D imaging system may suffer from severe vibrations and can use only one-period echoes for the ranging of each observation spot; consequently, it can provide only few measurement results. These vibrations may cause large errors because conventional vibration compensation methods are ineffective when applied to fast disturbances with limited measurement results. To solve this problem, we analyze the influence of vibrations on FMCW ladar ranging and propose a vibration compensation method based on an instantaneous ranging model for one-period triangular FMCW ladar signals. We first use a synchrosqueezing wavelet transform to extract time-frequency curves of the up- and down-dechirp signals and then build an instantaneous ranging model that can characterize local vibration errors. Based on the instantaneous ranges, we remove the disturbance vibration errors by taking the mean values of the instantaneous ranges and obtain the target range by using the triangular relations of the up and down observations. Experiments based on synthetic and real data verify the effectiveness of the proposed method and its superiority over the three-point method and Doppler shift method in compensating for vibrations with different frequencies and noise levels.Static Fourier transform spectrometers (S-FTSs) are well-consolidated instruments providing high throughput and high spectral resolution in a narrow spectral band. They use two reflective gratings as dispersive elements in a Michelson interferometer. Gratings allow high spectral dispersion and consequently high resolution, but, due to the light diffused from their grooves, they are one of the main noise sources in the reconstructed spectrum. In this work, we compare the signal-to-noise ratio performance of a prism-based S-FTS with that of a grating-based S-FTS. As a primary advantage, prisms give intrinsically lower diffused light than gratings. Furthermore, they do not have multiple diffracted orders, reducing thereafter the optical constraints on the instrumental baffling.
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