Notes

Immunosuppression seen as an improved Treg mobile or portable and IL-10 levels inside benzene-induced hematopoietic toxic body mouse design.
To develop and externally validate a computed tomography (CT)-based radiomics model for predicting lymphovascular invasion (LVI) before treatment in patients with rectal cancer (RC).

This retrospective study enrolled 351 patients with RC from three hospitals between March 2018 and March 2021. These patients were assigned to one of the following three groups training set (n=239, from hospital 1), internal validation set (n=60, from hospital 1), and external validation set (n=52, from hospitals 2 and 3). Large amounts of radiomics features were extracted from the intratumoral and peritumoral regions in the portal venous phase contrast-enhanced CT images. The score of radiomics features (Rad-score) was calculated by performing logistic regression analysis following the L1-based method. A combined model (Rad-score+clinical factors) was developed in the training cohort and validated internally and externally. The models were compared using the area under the receiver operating characteristic curve (AUC).

Of the 351 patients, 106 (30.2%) had an LVI+tumor. Rad-score (comprised of 22 features) was significantly higher in the LVI+group than in the LVI- group (0.60±0.17 vs. 0.42±0.19, P=0.001). The combined model obtained good predictive performance in the training cohort (AUC=0.813 [95% CI 0.758-0.861]), with robust results in internal and external validations (AUC=0.843 [95% CI 0.726-0.924] and 0.807 [95% CI 0.674-0.903]).

The proposed combined model demonstrated the potential to predict LVI preoperatively in patients with RC.
The proposed combined model demonstrated the potential to predict LVI preoperatively in patients with RC.Auditory neurons connect the sensory hair cells from the inner ear to the brainstem. These bipolar neurons are relevant targets for pharmacological intervention aiming at protecting or improving the hearing function in various forms of sensorineural hearing loss. In the research laboratory, neurotrophic compounds are commonly used to improve survival and to promote regeneration of auditory neurons. One important roadblock delaying eventual clinical applications of these strategies in humans is the lack of powerful in vitro models allowing high throughput screening of otoprotective and regenerative compounds. GW2580 The recently discovered auditory neuroprogenitors (ANPGs) derived from the A/J mouse with an unprecedented capacity to self-renew and to provide mature auditory neurons offer the possibility to overcome this bottleneck. In the present study, we further characterized the new phoenix ANPGs model and compared it to the current gold-standard spiral ganglion organotypic explant (SGE) model to assay neurite outgrowth, neurite length and glutamate-induced Ca2+ response in response to neurotrophin-3 (NT-3) and brain derived neurotrophic factor (BDNF) treatment. Whereas both, SGEs and phoenix ANPGs exhibited a robust and sensitive response to neurotrophins, the phoenix ANPGs offer a considerable range of advantages including high throughput suitability, lower experimental variability, single cell resolution and an important reduction of animal numbers. The phoenix ANPGs in vitro model therefore provides a robust high-throughput platform to screen for otoprotective and regenerative neurotrophic compounds in line with 3R principles and is of interest for the field of auditory neuroscience.To meet the emerging power demand of microelectronic and electronic skin based sensing platform, enzymatic fuel cells have received increasing attention due to their good human's compatibility, easy integration and cost effectiveness. Herein, we use multi-walled carbon nanotube/naphthoquinone to modify the lactate oxidase bio-anode to facilitate the electron transfer between electrocatalytic active site and electrode support. Polyvinyl alcohol hydrogel serves as the separator and lactate container. The bio-anode, Pt/C cathode and hydrogel are assembled in layer-by-layer structure, which can successfully utilize pre-stored and external lactate from human's sweat to generate the electricity. It delivers a power-density of 62.2 ± 2.4 μW cm-2 under bending/torsion conditions. Given that the broad substrate scope in sweat and easily assembled structure, it provides a plausible solution to power the miniaturized sensors and generic circuits.A bimodal 3D-electrochemiluminescence (ECL) analysis method was developed, which integrated simpleness, label-free, high-throughput and real time detection together. Firstly, a novel 3D copper-based nanosheet micro-material (Cu-Flo. NMs) coupled with gold nanoparticles/Cysteine (Cu-Flo.@AuNPs-Cys) was prepared to use as the versatile label for both colorimetric and ECL techniques. The 3D-Cu-Flo.@AuNPs-Cys having glucose oxidase-like activity could catalyze glucose to produce H2O2 in situ, which was further found to be capable of exhibiting a 30.95-fold higher ECL-intensity for luminol than bare glassy carbon electrodes (GCE). Taking advantages of the 3D-Cu-Flo.@AuNPs-Cys above, a colorimetric and ECL-channel sensor (GCE/3D-Cu-Flo.@AuNPs-Cys) were constructed simultaneously for glucose detection. The fabricated sensor displayed a wide linear range (Glucose 0.001-50 mmol L-1, AFP 2.25 × 10-7-225 ng mL-1), impressive low limit of detection (Glucose 1.27 × 10-7 mol L-1, AFP 1.92 × 10-8 ng mL-1, S/N = 3) and acceptable recovery (Glucose 94% ∼ 104%, AFP 96.04% ∼ 102.29%) in practical sample. Furthermore, the biosensor showed ultrafast (0.5 min) analysis efficiency, high stability for 6 cyclic potential scans and satisfactory reproducibility for 7 repeated tests. These results demonstrated the proposed 3D dual-modal ECL-biosensor for biomarkers detection had a great potential in clinical diagnostics, promoting the application in biomedical researching and POCT.Growing evidences reveal that Nrf2-mediated antioxidant defense response and mitophagy are involved in the toxic mechanism of heavy metals, but the effects of molybdenum (Mo) and cadmium (Cd) co-exposure on Nrf2-mediated antioxidant defense response and mitophagy in duck hypothalamus have yet to be elucidated. Herein, 40 healthy 7-day-old ducks were randomly assigned to 4 groups and fed diets containing different doses of Mo or/and Cd for 16 weeks, respectively. The data demonstrated that Mo or/and Cd notably elevated their contents in hypothalamus, decreased Cu, Fe, Zn and Se contents, caused pathological damage and oxidative stress accompanied by elevating MDA content and reducing CAT, T-AOC, T-SOD, GSH-Px activities. Moreover, Mo or/and Cd not only restrained Nrf2 pathway by decreasing Nrf2, HO-1, NQO1, GST, CAT, SOD1, GCLM mRNA expression levels and Nrf2 protein expression level, but also disturbed mitochondrial dynamics and triggered PINK1/Parkin-mediated mitophagy by enhancing MFF, PINK1, Parkin, Bnip3, LC3A, LC3B mRNA expression levels and PINK1, Parkin, LC3B-II/LC3B-I protein expression levels, inhibiting Mfn1, Mfn2, OPA1, P62 mRNA expression levels and P62 protein expression level, and facilitating the colocalization between LC3 and HSP60. The changes of above factors were most remarkable under Mo and Cd co-treatment. Overall, the results elucidate that Mo and Cd can synergistically inhibit Nrf2-mediated antioxidant defense response and activate PINK1/Parkin pathway-dependent mitophagy in duck hypothalamus, whose mechanism is somehow related to Mo and Cd accumulation.Algal ponds (APs) are widely used as treatment facilities for domestic sewage in sparsely populated rural areas. However, few AP studies have focused on daylight length to enhance pollutants removal. In this study, four algae ponds were set up, daylight was prolonged by 0, 2, 4, and 6 h with an illuminating intensity of 3000 lx. The highest removal efficiencies of total nitrogen, ammonium, and total phosphorus were 37.36%, 41.20%, and 21.56% due to the highest microbial abundance under optimum conditions (2 h PD), respectively. Excessive PD (4 h and 6 h) could inhibit the removal abilities. PD also increased the maximum relative electron transport rate of algae, leading to an increase in the photosynthetic capacity of APs. Meanwhile, the high microbial abundance indicates that chemoheterotrophic bacteria are the main influencing factor for the removal of nitrogen and phosphorus by the APs. Moreover, the system with PD using artificial lamps was proven to be feasible for engineering applications and potentially utilized in rural domestic wastewater treatment.The present study aims to examine the role of kisspeptin (KP), FSH, and its receptor (FSHR), and their interrelationships in the control of basic human ovarian granulosa cells functions. We investigated (1) the ability of granulosa cells to produce KP and FSHR, (2) the role of KP in the control of ovarian functions, and (3) the ability of KP to affect FSHR and to modify the FSH action on ovarian functions. The effects of KP alone (0, 10 and 100 ng/mL); or of KP (10 and 100 ng/mL) in combination with FSH (10 ng/mL) on cultured human granulosa cells were assessed. Viability, markers of proliferation (PCNA and cyclin B1) and apoptosis (bax and caspase 3), as well as accumulation of KP, FSHR, and steroid hormones, IGF-I, oxytocin (OT), and prostaglandin E2 (PGE2) release were analyzed by the Trypan blue exclusion test, quantitative immunocytochemistry, and ELISA. KP given at a low dose (10 ng/mL) stimulated viability, proliferation, inhibited apoptosis, promoted the release of progesterone (P4), estradiol (E2), I release, suggest that FSH influence these cells could be mediated by KP. Moreover, the capability of KP (100 ng/mL) to decrease FSHR accumulation, basal and FSH-induced ovarian parameters, suggest that KP can suppress some ovarian granulosa cell functions via down-regulation of FSHR. These observations propose the existence of the FSH-KP axis up-regulating human ovarian cell functions.Though endometriosis is benign, however, it shares certain characteristics with cancers, such as the ability to invade and metastasize. Previous studies have demonstrated that S-phase kinase associated protein2 (SKP2) promotes invasion, tumorigenesis, and metastasis. However, its correlation with adenomyosis is unclear. Herein, we aimed to look into SKP2 expression patterns and regulatory effects on endometrial stromal cell (ESC) proliferation and invasion, and its internal mechanism in adenomyosis. Western blot, qRT-PCR, and immunochemistry were carried out for detecting SKP2 and ZEB1 expression in ESC of adenomyosis and adenomyosis endometrial tissue. The primary ESCs were identified using immunofluorescence. SKP2 knockdown was accomplished in vitro by transfecting a particular lentivirus vector. The colony formation and CCK-8 assays were carried out for assessing cell proliferation, while cell invasion potential was assessed using the transwell assay. Both SKP2 and ZEB1 were found to be significantly upregulated in adenomyosis endometrial tissue. Knockdown of SKP2 inhibited adenomyotic ESC invasion and proliferation. Further experiments showed that knocking out SKP2 reduced ZEB1 expression in adenomyotic ESCs. Our results showed that SKP2 could regulate ZEB1 expression, and increased SKP2 may play a role in the pathogenesis of adenomyosis and stimulating ESC proliferation and invasion.
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