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Ni-NTNW electrodes and hydroxide-decorated variants showed excellent performance in glucose sensing. The highest activity was achieved for the catalyst augmented with NiCo-LDH nanosheets, which surpassed the modification with pure Ni(OH)2. Despite its low thickness of 20 µm, the optimized catalyst layer provided an outstanding sensitivity of 4.6 mA mM-1 cm-2, a low detection limit of 0.2 µM, a fast response time of 5.3 s, high selectivity and stability, and two linear ranges covering four orders of magnitude, up to 2.5 mM analyte. As such, derivatized interconnected metal nano-networks represent a promising design paradigm for highly miniaturized yet effective catalyst electrodes and electrochemical sensors.A binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time-of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl, N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important detection parameter. The enhancing effect of carbon dots was linked to a negative Gibbs energy of the complex formation between CDs, Na+, cholesterol and DHB, and it was supported by theoretical calculations. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical excitation, dipole moment and oscillator strength positively affected the cholesterol detection by MALDI in the presence of Na+.Pollution caused due to discharge of toxic and hazardous chemical contaminants from industrial processes is an issue of major environmental concern. Hexavalent chromium [Cr(VI)] is one such known toxic heavy metal contaminant emanated largely from various industrial processes. Since physical-chemical treatment techniques are beset with several problems, there is an increased attention on the use of waste biomaterials/biomass as sorbents for the elimination of heavy metals from aqueous matrices. The main purpose of this study was to evaluate the effectiveness of some low-cost waste biomaterials such as fruit wastes, agricultural and industrial waste/byproducts, waste parts of photosynthetic plants, aquatic plants and fungal biomass collected from different sources for the biosorption of Cr(VI) from aqueous matrices. Amid the tested biomaterials, wood apple shell (WAS) biomass (Limonia acidissima) was found to be highly efficient biosorbent for Cr(VI) sorption. selleck compound In majority of biomass, it was observed that biosorption of Cr(VI) took place at acidic pH with optimum pH ranging from 2.0 to 5.0. Loading capacity of WAS biomass (29.37 mg/g) was higher than that of conventional adsorbent activated charcoal (26.56 mg/g), which was used as control. Cr(VI) treated biomass (WAS) was characterized using instrumental techniques such as Scanned Electron Microscopy (SEM) and Energy Dispersive X-ray (EDX) confirmed the adsorption of Cr(VI). Boehm titration and FTIR studies were conducted to ascertain the presence of functional groups responsible for Cr(VI) sorption by WAS biomass. The WAS biomass removed Cr(VI) from industrial wastewater with an efficiency of >99.9% thus complying with the statutory limits. Considering the economical aspect, the selected biomass can be viewed as a potential candidate for the elimination of toxic contaminant from wastewater.The group of Insect-specific viruses (ISVs) includes viruses apparently restricted to insects based on their inability to replicate in the vertebrates. Increasing numbers of ISVs have been discovered and characterized representing a diverse number of viral families. However, most studies have focused on those ISVs belonging to the family Flaviviridae, which highlights the importance of ISV study from other viral families, which allow a better understanding for the mechanisms of transmission and evolution used for this diverse group of viruses. Some ISVs have shown the potential to modulate arboviruses replication and vector competence of mosquitoes. Based on this, ISVs may be used as an alternative tool for biological control, development of vaccines, and diagnostic platforms for arboviruses. In this review, we provide an update of the general characteristics of ISVs and their interaction with arboviruses that infect vertebrates.How BRCA1 germline mutations predispose to cancer remains poorly understood. Induced pluripotent stem cells (iPSCs) represent an emerging model to investigate the molecular mechanisms underlying malignant transformation in primary cells from individuals who are carriers of deleterious mutations in the BRCA1 gene. Here we report the generation and characterization of iPSC lines from a female donor harboring a germline c.3612delA mutation in the BRCA1 gene and her daughter who does not carry the mutation. Skin fibroblasts were reprogrammed using non-integrative Sendai virus and characterized for their pluripotent properties. These iPSCs are a valuable cellular model for personalized pre-clinical research in the context of BRCA1 mutant hereditary cancers.Antley-Bixler syndrome (ABS) is a rare inherited autosome recessive malformation syndrome, which can be caused by the gene mutations of cytochrome P450 oxidoreductase (POR). In this study, the urine cells (UCs) derived from a 5-year-old female ABS patient with the homozygote POR gene mutation p.R457H (c.1825C>G) were reprogramming into induced pluripotent stem cells (iPSCs) named WMUi018-A using a commercial Sendai virus reprogramming kit. The pluripotent markers of stem cells like OCT4 and SOX2 can be positively expressed in this iPSC line, which can be induced to differentiate into three germ layers in vitro and maintain a stable karyotype (46, XX).
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