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This study aimed to obtain a reliable evaluation about addition of tert-butylhydroquinone (TBHQ), and distribution of TBHQ and 2-tert-butyl-1,4-benzoquinone (TBBQ) contents in typical edible oils and oleaginous foods marketed in Hangzhou City. Briefly, the probability of labeled with addition of TBHQ in foods decreased from 36.45 ± 2.6% to 28.78 ± 3.7% in the period from 2018 to 2020. In the 135 analyzed samples, TBHQ contents were far less than the maximum legal additive amount, and TBBQ contents ranged from below its limit of quantification (LOQ) to 13.54 ± 1.15 mg/kg. The conversion rate from TBHQ to TBBQ in edible oils was 2.94 ± 1.17%, much lower than that in other food categories. Further research determined that the process method and food composition were the main factors for different conversion rates from TBHQ to TBBQ in various food categories. In addition, oil consumption was found to be the primary source of dietary intake of TBHQ and TBBQ.Rice quality changes during storage, but there have been few studies of how rice proteins changes during aging. The present study characterized the structural properties of protein in stored rice and identified the mechanism of quality deterioration using proteomics. Compared with protein from newly harvested rice, the free sulfhydryl content of protein from stored rice was significantly reduced and the disulfide bond content and surface hydrophobicity was higher. Storage resulted in a loss of α-helix and β-sheet structures and increase in β-turn and random coil structures. High-molecular-weight protein subunits decomposed to produce low-molecular-weight subunits at 30 °C, while protein aggregation occurred at 70 °C. At 30 ℃ 157 differential proteins were found and 70 ℃ 395 such proteins occurred. Redox homeostasis, response to oxidative stress, glutathione metabolism, tricarboxylic acid cycle, glycolysis/gluconeogenesis, starch and sucrose metabolism, and fatty acid biosynthesis and degradation led to the different quality of stored rice.In the present study, we aimed to clarify features of carcinomatous cirrhosis from breast cancer presenting as refractory transudate ascites and acute liver failure. check details In our systematic literature review, we identified 26 studies and 31 cases including our case of this rare condition. Our patient was a 49-year-old woman with a history of ascites and liver failure for the past 4 years and currently being treated for invasive ductal breast cancer. On radiography, she had occult liver metastases that were confirmed using laparoscopic liver biopsy. In the 31 cases, data on the reported year, age, type of primary breast cancer, time from breast cancer diagnosis, presence of ascites and/or varices, liver biopsy, diagnostic modalities, outcomes, and survival were documented and analyzed. All cases were reported during 1984-2020, with a mean patient age of 52.9 years. Eighteen patients (58.1%) were diagnosed with ductal breast cancer. Twenty-two patients (70.9%) had ascites. All patients had gradual progression to liver dysfunction. The following tests were performed computed tomography (77.4%); ultrasound (58.0%); liver biopsy (100%); postmortem biopsy (35.5%), transjugular liver biopsy (32.3%), and laparoscopic liver biopsy (3.2%). Outcomes were reported for 29 patients, of whom 24 (82.3%) died after 1 day to 16 months. Invasive ductal carcinoma was the most common histological type; however, invasive lobular carcinoma was more frequent (32.3%) than its reported incidence in the breast. Carcinomatous cirrhosis has poor prognosis at relatively rash and is difficult to diagnose with usual modalities. It may be associated with E-cadherin loss or CD44 pronouncement.Endo-β-1,3-glucanase with high specific activity is a prerequisite for enzymatic preparation of valuable β-oligoglucosides. Heterologous expression in Pichia pastoris GS115 with error-prone PCR technology was implemented, and the mutant strain 7 N12 was obtained. The mutant endo-β-1,3-glucanase showed efficient specific activities for degrading curdlan (366 U mg-1) and scleroglucan (274.5 U mg-1). Thereafter, one-step production of functional branched oligoglucosides was established with coupled fermentation of Pichia pastoris and Sclerotium rolfsii. During the fermentation process, the endo-β-1,3-glucanase secreted by Pichia pastoris GS115 can efficiently hydrolyse scleroglucan metabolized by Sclerotium rolfsii WSH-G01. The maximum yields of β-oligoglucosides in the shake flasks and 7-L bioreactor reached 1.73 g L-1 and 12.71 g L-1, respectively, with polymerization degrees of 2-17. The successful implementation of heterologous expression with error-prone PCR and the coupled fermentation simplified the multi-step enzymatic β-oligoglucoside preparation procedures, which makes it a potential strategy for industrial production of functional oligosaccharides.In this study, the influence of distinct hydraulic retention times (HRT) and organic loading rates (OLRs) on fungal dynamics during food waste anaerobic digestion in immersed membrane-based bio-reactors (iMBR) were investigated. The organic loading rate 4-8 g VS/L/d (R1) and 6-10 g VS/L/d (R2) were set in two iMBR. T1 (1d), T2 (15d) and T3 (34d) samples collected from each bioreactor were analyzed fungal community by using 18s rDNA. In R2, T2 had the most abundant Ascomycota, Basidiomycota, Chytridiomycota and Mucoromycota. As for R1, T3 also had the richest Cryptomycota except above four kinds of fungi. Subsequently, the Principal Component Analysis (PCA) and Non-Metric Multi-Dimensional Scaling (NMDS) indicated that fungal diversity was varied among the all three phases (T1, T2, and T3) and each treatment (R1 and R2). Finally, the results showed that different OLRs and HRT have significantly influenced the fungal community.Poultry slaughterhouse wastewater (PSW) contains high organic matter and nutrients requiring thus a special treatment before its final disposal. In this work, acid precipitation (H2SO4) followed by microalgae "Chlorella vulgaris" cultivation both in batch and continuous processes was studied as an alternative method for PSW treatment. By reducing the pH value of PSW from 6 to 7 to 4, about 80% of the total chemical oxygen demand (CODT) was removed as sludge. In the supernatant, the COD residual was efficiently removed (83%) by microalgae in the batch process, using an internal-loop concentric tube photobioreactor (4.5 L). Moreover, in continuous process, after 89 h, the COD value resulted lower than 200 mg L-1 and 1.2 g L-1 of microalgae in the output line. The proposed PSW treatment method is promising from economic and environmental viewpoints, since the microalgal biomass can be valued in a biorefinery context.
Read More: https://www.selleckchem.com/products/estradiol-benzoate.html
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