Notes

Projecting preventable healthcare facility events inside Md.
Little is known about the individual differences in susceptibility to, or lifetime frequency of clinically distinguishable influenza in children.

Rapid enzyme linked immunoassay-confirmed influenza pediatric cases (n = 96) in season 1 (2017-2018) were compared to age-matched (mean 7.7 years) controls (n = 171) with no evidence of influenza in season 1. The 2 cohorts were again studied in season 2 (2018-2019) for influenza outcomes and influences. Medical records, questionnaires, and interviews were used to determine past influenza disease and vaccine histories.

After season 2, known lifetime influenza illnesses per year of age averaged 22.6% in cases and 5.6% in controls, with 62% of controls still having never experienced known influenza. Having had prior influenza was marginally significant as a risk for season 1 influenza in cases versus controls (
 = .055), yet a significant risk factor in controls for season 2 (
 = .018). Influenza vaccine rates were significantly higher in controls than in casesclinical influenza and current risk, point to innate differences in individual influenza experiences.[This corrects the article DOI 10.1155/2019/5134190.].
This study aims to assess the corneal epithelial remodeling within a 9 mm diameter zone induced by small incision lenticule extraction (SMILE) and evaluate its relationship with the refractive outcomes.

A total of 64 eyes of 64 patients were included. Wide-field optical coherence tomography (OCT) was used to measure the epithelial thickness (ET) across a 9 mm diameter area, preoperatively, and after one day, one week, one month, three months, and six months postoperatively. The ET changes were compared among the different time points and analyzed zones.

The ET increases from one week to three months and stabilized from three months to six months. Compared to the preoperative values, the mean ET changes at six months in central (2 mm), paracentral (2-5 mm), mid-peripheral (5-7 mm), and peripheral (7-9 mm) zones were 4.37, 4.36, 1.61, and -1.59 
m, respectively. The correlation between the epithelial thickening and the amount of myopia correction was positive in central (
 = 0.001) and paracentral zones (
 < 0.001) and negative in peripheral zone (
 = 0.006). The intended diameter of the optical zone was negatively related to epithelial hyperplasia in the central (
 = 0.020) and paracentral zone (
 = 0.006), and the correlation was positive in the mid-peripheral zone (
 = 0.001). The epithelial thickening of central zone (
 = 0.012) and the difference of mean ET between central and paracentral zone (
 = 0.020) were negatively related to the spherical equivalent at six months.

An asymmetric lenticule-like pattern of epithelial remodeling occurred in 9 mm diameter cornea at six months after myopic SMILE. The epithelial remodeling may affect the refractive outcomes of SMILE.
An asymmetric lenticule-like pattern of epithelial remodeling occurred in 9 mm diameter cornea at six months after myopic SMILE. The epithelial remodeling may affect the refractive outcomes of SMILE.As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic continues, other previously ignored viruses must be taken into account as causes of severe acute respiratory distress, influenza-like illness and pneumonia. In this article, we report two cases of pneumonia in chronic liver disease patients where human coronavirus (HCoV) 229E was identified as the only infecting pathogen. Both the patients presented with fever, cough and respiratory distress, along with radiological findings suggestive of pneumonia. read more Multiplex real-time PCR for various respiratory viruses (FilmArray Respiratory Panel 2 plus) detected HCoV-229E in both cases. Both cases were managed with prophylactic antibiotics, steroids and supplemental oxygen therapy, after which they recovered completely and were discharged.Plant-based protein is being sought after as a substitute for fish meals (powdered fish) in tilapia feeds. This is to promote sustainable aquaculture, as fish meals contribute to the dwindling marine fish catch. Amaranthus spinosus is an edible weed that shows potential to improve the growth and immunity of Nile tilapia. However, most studies only consider the survivability of fish to evaluate the benefit of using plant-based feeds and do not necessarily elucidate whether a pathogen is affected in vivo. A. spinosus leaf meals (ASLMs) were used to determine effectiveness against Aeromonas hydrophila (BIOTECH 10089) injected intraperitoneally into Nile tilapia. Formulated feeds with fish meals substituted with 50 % (ASLM50) and 75 % (ASLM75) A. spinosus leaves were fed to Nile tilapia challenged with A. hydrophila . Then spleen and kidney tissue were collected and analysed 10 days post-injection for total plate count. The fish fed with ASLM50 appeared healthier than those fed with ASLM75 and those fed with control feeds. Fish fed with ASLMs had lower A. hydrophila counts (P=0.03). Phytochemical screening and antimicrobial activity determination for crude methanolic A. spinosus leaf (ASL) and ASLMs were also conducted to enhance the in vivo results. The metabolites present in the extracts were carbohydrates, amino acids and proteins, cardiac glycosides, saponins and terpenoids. The ASL and ASLM extracts had antimicrobial activity (MIC=115 mg ml-1). Overall, the study showed that ASLMs can make tilapia more resilient against A. hydrophila infections. Fish meal substitution was best at 50 %. Higher substitution had unwanted effects (more bacterial counts), possibly due to antinutritional factors.Tall fescue KY-31 is an important primary forage for beef cattle. It carries a fungal endophyte that produces ergovaline, the main cause of tall fescue toxicosis that leads to major revenue loss for livestock producers. The MaxQ, an engineered cultivar, hosts an ergovaline nonproducing strain of the fungus and consequently is nontoxic. However, it is less attractive economically. It is not known how rumen microbiome processes these two forages towards nutrient generation and ergovaline transformation. We have analysed the rumen microbiome compositions of cattle that grazed MaxQ with an intervening KY-31 grazing period using the 16S rRNA-V4 element as an identifier and found that KY-31 remodelled the microbiome substantially, encompassing both cellulolytic and saccharolytic functions. The effect was not evident at the whole microbiome levels but was identified by analysing the sessile and planktonic fractions separately. A move from MaxQ to KY-31 lowered the Firmicutes abundance in the sessile fraction and inc It highlighted the importance of analysing sessile and planktonic fractions separately.New insights in evolution are available thanks to next-generation sequencing technologies in recent years. However, due to the network of complex relations between species, caused by the intensive horizontal gene transfer (HGT) between different bacterial species, it is difficult to discover bacterial evolution. This difficulty leads to new research in the field of phylogeny, including the gene-based phylogeny, in contrast to sequence-based phylogeny. In previous articles, we presented evolutionary insights of Synteny Index (SI) study on a large biological dataset. We showed that the SI approach naturally clusters 1133 species into 39 cliques of closely related species. In addition, we presented a model that enables calculation of the number of translocation events between genomes based on their SI distance. Here, these two studies are combined together and lead to new insights. A principal result is the relation between two evolutionary clocks the well-known sequence-based clock influenced by point mutations, and SI distance clock influenced by translocation events. A surprising linear relation between these two evolutionary clocks rising for closely related species across all genus. In other words, these two different clocks are ticking at the same rate inside the genus level. Conversely, a phase-transition manner discovered between these two clocks across non-closely related species. This may suggest a new genus definition based on an analytic approach, since the phase-transition occurs where each gene, on average, undergoes one translocation event. In addition, rare cases of HGT among highly conserved genes can be detected as outliers from the phase-transition pattern.Mycolicibacterium fortuitum is a fast-growing bacterium and an opportunistic pathogen implicated in human and animal infections. Here we report the first case and genetic characterization of a strain of M. fortuitum isolated from skin lesions of a companion cat with atypical cutaneous mycobacteriosis in Brazil. In addition, the genome of this strain was sequenced, representing the first genome of this opportunistic pathogen isolated from an animal infection. The in silico and in vitro analysis regarding antibiotic resistance of this strain showed an intrinsic multiresistance antibiotic profile. However, this strain showed sensitivity to amikacin and ciprofloxacin, and the cat was treated long-term with these drugs.Cyclospora cayetanensis is a parasite that causes intestinal disease that can be especially severe in immunocompromised patients. Most cases occur in tropical and subtropical areas, and in industrialized countries their diagnosis is mostly linked to international travel or the ingestion of imported food. We describe this case of severe diarrhoea in a patient with diffuse large B cell lymphoma and no epidemiological risk factors that was successfully treated with trimethoprim-sulfamethoxazole (TMP-STX). C. cayetanensis is a pathogen that should be taken into account in patients with chronic diarrhoea, especially immunocompromised patients, even when no epidemiological risk factors are present.
infections are being increasingly reported in both immunocompetent and immunocompromised patients. We describe a case of
infection with an atypical presentation in an immunocompetent patient.

A previously healthy 47-year-old gentleman presented with hiccups and paroxysmal spasms. Imaging revealed a pulmonary nodule, for which he underwent surgical resection. Pathologic evaluation demonstrated evidence of local inflammation, with growth of
on tissue cultures.

may have atypical presentations in immunocompetent patients. Further research is needed to understand the factors leading to
infections in immunocompetent patients.
Nocardia abscessus may have atypical presentations in immunocompetent patients. Further research is needed to understand the factors leading to Nocardia infections in immunocompetent patients.Bacteriophages (phages) are viruses that target bacteria, with the ability to lyse and kill host bacterial cells. Due to this, they have been of some interest as a therapeutic since their discovery in the early 1900s, but with the recent increase in antibiotic resistance, phages have seen a resurgence in attention. Current methods of isolation and purification of phages can be long and tedious, with caesium chloride concentration gradients the gold standard for purifying a phage fraction. Isolation of novel phages requires centrifugation and ultrafiltration of mixed samples, such as water sources, effluent or faecal samples etc, to prepare phage filtrates for further testing. We propose countercurrent chromatography as a novel and alternative approach to use when studying phages, as a scalable and high-yield method for obtaining phage fractions. However, the full extent of the usefulness and resolution of separation with this technique has not been researched; it requires optimization and ample testing before this can be revealed.
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