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This study was aimed to investigate whether 1-deoxynojirimycin (DNJ) affects the digestion system of young geese and assess whether mulberry leaf, which contains this substance, has disadvantages that compromise its value as poultry feed. One hundred and twenty-eight 12-day-old male Wanxi white geese were randomly assigned into 4 treatment groups. The control group was fed an ordinary diet without DNJ. The other groups namely L-DNJ, M-DNJ, and H-DNJ had their basic diets supplemented with 0.05 mg/g, 0.1 mg/g, and 0.15 mg/g DNJ, respectively. The geese were fed for 6 wk, and the apparent digestibility test was conducted in the last week. Intestinal parameters, digestive organs, and enzymes were determined. 16S rRNA gene sequencing was conducted for cecal flora composition. The results revealed that DNJ decreased body and liver weight and increased feed conversion ratio in comparison with the control (P 0.05) in the pancreas and duodenum. The intestinal villi were short, even impaired, in DNJ-treated groups. High-throughput sequencing data revealed that DNJ supplement reduced the α-diversity indices of the cecal microbiota. The principal component analysis further suggested a difference in community structure between the DNJ treatment groups and control. High-dose DNJ increased the relative abundance of Bacteroides, Escherichia-Shigella, and Butyricicoccus but reduced that of unclassified Ruminococcaceae compared with the control (P less then 0.05). In conclusion, changes in the digestive system caused by DNJ seriously affected the metabolism of nutrients in geese and reduced their growth performance. Attention should be paid to the adverse effects of DNJ when using mulberry leaves as poultry feed.This study evaluated the effects of varying levels of L-arginine (Arg) on performance and intestinal health of broilers challenged with Eimeria. Cobb 500 male chicks (n = 720) were randomly distributed in a 5 × 2 factorial arrangement (6 replicates/12 birds). The main factors were Arg levels (1.04, 1.14, 1.24, 1.34, 1.44%) and challenge or non-challenge with Eimeria. At day 12, in the challenge group, each bird received orally 12,500 Eimeria maxima, 12,500 Eimeria tenella, and 62,500 Eimeria acervulina sporulated oocysts. At 5 d postinfection (dpi), intestinal permeability was measured. At 6 and 14 dpi, performance, intestinal histomorphology, nutrient digestibility, tight junction protein (TJP) gene expression, and antioxidant markers were evaluated. Few interactions were found, and when significant, the supplementation of Arg did not counteract the negative effects of Eimeria challenge. Tacrolimus Challenge, regardless of Arg level, increased intestinal permeability, although the expression of Claudin-1, a TJP, was uperall growth, intestinal integrity, and morphology in broilers subjected or not to Eimeria challenge.The present study proposes a Gaussian process regression (GPR) approach to develop a model to predict true metabolizable energy corrected for nitrogen (TMEn) content of corn samples (as model output) for poultry given levels of feed chemical compositions of crude protein, ether extract, crude fiber, and ash (as model inputs). A 30 corn samples obtained from 5 origins [Brazil (n = 9), China (n = 5), Iran (n = 7), and Ukraine (n = 9)] were assayed to determine chemical composition and TMEn content using chemical analyses and bioassay technique. In addition to GPR model, data were also analyzed by multiple linear regression (MLR) model. Results revealed that corn samples of different origins differ in their gross energy and chemical composition of crude protein, crude fiber, and ash, but no differences were observed for their ether extract and TMEn contents. Based on model evaluation criteria of R2 and root mean square error (RMSE), the GPR model showed satisfactory performance (R2 = 0.92 and RMSE = 33.68 kcal/kg DM) in predicting TMEn and produced relatively better prediction values than those produce by MLR (R2 = 0.23 and RMSE = 104.85 kcal/kg DM). The GPR model may be capable of improving our aptitude and capacity to precisely predict energy contents of feed ingredients to formulate optimal diets for poultry.This study investigated the effects of dietary corn-resistant starch on lipid metabolism of broilers and its potential relationship with cecal microbiota modulation. A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively). Each group had 8 replicates with 8 broilers per replicate. The experiment lasted 21 d. The results showed that the abdominal fat percentage were lower in birds from 8%RS and 12%RS groups (0.75 and 0.58%, respectively) than those from NC and CS groups (1.20 and 1.28%, respectively; P less then 0.05). The birds from 8%RS and 12%RS groups exhibited lower concentrations of blood triglyceride and nonestesterified fatty acid than those in the NC and CS groups (P less then 0.05). Moreover, birds fed die profound understanding about the relationship between gut microbial composition and lipid metabolism in broilers.The present research was conducted to assess Mn requirements of broiler breeder hens. One hundred and twenty Cobb 500 hens, 22 wk of age, were individually allocated in cages. After fed a Mn-deficient diet (22.2 ppm), hens were randomly placed in treatments having 6 increments of 30-ppm Mn. All trace minerals were from laboratory grade sources being Mn from Mn sulfate (MnSO4H2O). Treatments were fed for 4 periods of 28 d. There were no interactions between dietary Mn and period for any evaluated response (P > 0.05). Requirements of Mn for hen day egg production and settable egg production were 115.8 and 56.6 ppm and 122.1 and 63.6 ppm (P less then 0.05), respectively, using quadratic polynomial (QP) and broken line quadratic (BLQ) models, whereas total eggs and total settable eggs per hen had Mn requirements estimated at 115.7 and 56.6 and 121.8 and 61.7 ppm (P less then 0.05), respectively. Number of cracked, defective, and contaminated eggs decreased, whereas hatchability, hatchability of fertile eggs, eggshell percentage, and eggshell palisade layer increased when hens were fed diets having 48.
Homepage: https://www.selleckchem.com/products/FK-506-(Tacrolimus).html
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