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MicroRNA-324-3p Plays A safety Position Against Coxsackievirus B3-Induced Virus-like Myocarditis.
nting with genetic variants that offer an explanation for otherwise uncommon symptom combinations but yet are of unknown significance.While convolutional neural networks (CNNs) continue to renew state-of-the-art performance across many fields of machine learning, their hardware implementations tend to be very costly and inflexible. Neuromorphic hardware, on the other hand, targets higher efficiency but their inference accuracy lags far behind that of CNNs. To bridge the gap between deep learning and neuromorphic computing, we present bitstream-based neural network, which is both efficient and accurate as well as being flexible in terms of arithmetic precision and hardware size. Our bitstream-based neural network (called SC-CNN) is built on top of CNN but inspired by stochastic computing (SC), which uses bitstreams to represent numbers. Being based on CNN, our SC-CNN can be trained with backpropagation, ensuring very high inference accuracy. At the same time our SC-CNN is deterministic, hence repeatable, and is highly accurate and scalable even to large networks. Our experimental results demonstrate that our SC-CNN is highly accurate up to ImageNet-targeting CNNs, and improves efficiency over conventional digital designs ranging through 50-100% in operations-per-area depending on the CNN and the application scenario, while losing less then 1% in recognition accuracy. In addition, our SC-CNN implementations can be much more fault-tolerant than conventional digital implementations.Structural segmentation of T1-weighted (T1w) MRI has shown morphometric differences, both compared to controls and longitudinally, following a traumatic brain injury (TBI). While many patients with TBI present with abnormalities on structural MRI images, most neuroimaging software packages have not been systematically evaluated for accuracy in the presence of these pathology-related MRI abnormalities. The current study aimed to assess whether acute MRI lesions (MRI acquired 7-71 days post-injury) cause error in the estimates of brain volume produced by the semi-automated segmentation tool, Freesurfer. More specifically, to investigate whether this error was global, the presence of lesion-induced error in the contralesional hemisphere, where no abnormal signal was present, was measured. A dataset of 176 simulated lesion cases was generated using actual lesions from 16 pediatric TBI (pTBI) cases recruited from the emergency department and 11 typically-developing controls. Simulated lesion cases were compared toanalyses address the focal region where the lesion was located however, our results suggest that focal correction approaches are insufficient for the global error in morphometric measures of the injured brain.Toxoplasmosis is one of the most important zoonotic diseases with serious health risks for humans, especially for immunodeficient patients, and can lead to abortion in pregnant women worldwide. The oral uptake of sporulated oocysts and/or consumption of undercooked/raw meat of animals infected with Toxoplasma gondii can infect other animals and humans. Heart, liver, and meat tissues of 150 sheep and 150 goats from a slaughterhouse in Ahvaz, Iran, were collected during autumn 2018 and analyzed via polymerase chain reaction (PCR) to detect parasitic DNA in the animal tissues. Moreover, antibodies against T. gondii of 150 sera samples were detected as the targets by in-house enzyme-linked immunosorbent assay (in-house ELISA). A total of 26 (17.3%), 33 (22%), and 48 (32%) of liver, meat, and heart samples in sheep, and a total of 24 (16%), 26 (17.3%), and 36 (24%) of liver, meat, and heart samples in goats, respectively, showed positive PCR results. Besides, the ELISA evaluation of sera samples from 150 sheep and 150 goats resulted in 26 (13.3%) and 16 (10.6%) positive cases, respectively. A significant difference was also found between PCR-positive heart samples and ELISA-positive sera samples of both animal species (p  0.05). The results of this study confirm the presence of T. gondii in sheep and goats' consumable organs, highlighting the need to avoid consuming raw or uncooked organs of these animal species to prevent human infection with T. gondii.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), can lead to severe respiratory illness. Patients with underlying comorbidities have a high risk of contracting COVID-19. Therefore, serological assays are urgently needed to diagnose asymptomatic carriers of SARS-CoV-2, to estimate the prevalence of infection, and for disease prevention and control. This study aimed to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of anti-SARS-CoV-2 antibodies in humans.

An ELISA test was designed and established to detect antibodies against the SARS-CoV-2 spike protein in serum samples from 41 quantitative reverse transcription polymerase chain reaction (qRT-PCR) - positive hospitalised COVID-19 patients. Forty-two convalescent patients' sera served as positive controls, while 117 pre-pandemic serum samples were used as negative controls.

A comparison between different SARS-CoV-2 proteins was performed, which included the fuion level and may be used for in future seroepidemiological studies.Simple and low-cost solutions are becoming extremely important for the evolving necessities of biomedical applications. Even though, on-chip sample processing and analysis has been rapidly developing for a wide range of screening and diagnostic protocols, efficient and reliable fluid manipulation in microfluidic platforms still require further developments to be considered portable and accessible for low-resource settings. In this work, we present an extremely simple microfluidic pumping device based on three-dimensional (3D) printing and acoustofluidics. The fabrication of the device only requires 3D-printed adaptors, rectangular glass capillaries, epoxy and a piezoelectric transducer. selleck inhibitor The pumping mechanism relies on the flexibility and complexity of the acoustic streaming patterns generated inside the capillary. Characterization of the device yields controllable and continuous flow rates suitable for on-chip sample processing and analysis. Overall, a maximum flow rate of ~ 12 μL/min and the control of pumping direction by frequency tuning is achieved.
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