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Subcellular localization demonstrated that turbot SRB1 was distributed in the membrane and cytoplasm. rSmSRB1 showed binding ability in vitro to bacteria, LPS, PGN, LTA and virus. Protein-protein interaction network agrees the function of SRB1 as lipoprotein receptor. Our results indicated SmSRB1 might act as co-receptors to TLRs and NLRs to modulate the immune response to pathogens. Further studies should pay attention to evaluate the specific co-receptor for SRB1 in recognition of different pathogens and selective mechanisms involved. The geoduck Panopea globosa is an endemic and economic valuable species from the Mexican Northwest coast whose biology has been little studied. No information exists about their hemocytes to date, which is highly important to assess the welfare of wild and cultured organisms. In this study, hemocytes of adult P. globosa were characterized at the morphological, ultrastructural and functional level. The mean number of hemocytes in the hemolymph of P. globosa was 6 × 105 ± 2 × 105 cells mL-1. The cells were identified as granulocytes (Gr) and hyalinocytes (H). The former accounted for 28% of adhered cells in the hemolymph, measured 6-18 μm, showed numerous basophilic granules in the cytoplasm, with round and eccentric nuclei, and a nucleuscytoplasm ratio of 0.44 ± 0.01. Hyalinocytes were the most abundant cells in the hemolymph of P. globosa (72% adhered cells) and were subdivided, according to their size, in small (Hs) 4-12 μm and large (HL) 6-18 μm. Hyalinocytes were eosinophilic round or ovoid cells with a central or eccentric nucleus, few or no granules in the cytoplasm and similar nucleuscytoplasm ratio (Hs 0.63 and HL 061). Lysosomes and lipids were observed in Gr, while carbohydrates were the most abundant energy substrate in H. Both hemocytic cell types, mainly Gr, were capable to ingest particles and yield superoxide (P > 0.05). The present study shows for the first time the cell types, abundance and immune activities of hemocytes present in the hemolymph of P. globosa. This information provides a useful baseline to carry out further research on the cellular immune response of the clam to potential pathogens or changes in environmental factors. PURPOSE To investigate the individual and combined contributions of acetabular and femoral morphology on hip range of motion (ROM) in patients with femoroacetabular impingement syndrome (FAIS) using computed tomography (CT) measurements and hip ROM evaluated on physical examination. METHODS A retrospective chart and radiographic analysis of patients presenting with hip pain suggestive of FAIS was performed. Femoral neck-shaft angle, femoral version, magnitude and clock-face location of the alpha angle, mid-coronal center edge angle (CEA), mid-sagittal CEA, acetabular version and McKibbin index were measured on CT scan. Univariate and multivariate linear regression analyses determined which measurements correlated with hip ROM including hip flexion as well as hip internal and external rotation with the hip in 90 degrees of flexion. RESULTS Two hundred hips were included in the analysis that met inclusion and exclusion criteria during the time period of eligibility. The mean age was 31.9 ±10.0 years, 145 (72%) sty or osteotomy. To instill quality in published clinical research, reporting guidelines, consisting of checklists and flowcharts, were developed to protect against reporting poorly designed research and researchers should be aware of the available instruments and their appropriate use. With the popularity of synthetic reviews, meta-analysis, and systematic reviews, there is a greater need to assess risk of bias and study quality. This review highlights the most frequently used guidelines and checklists, risk of bias scales, and quality rating scales that can assist the researcher with improving their research and its eventual publication. PURPOSE The purpose of this study was to develop and validate a standardized patient satisfaction measurement tool for adult patients undergoing primary anterior cruciate ligament reconstruction. METHODS A four-phase iterative process that included item generation and pilot survey development, item reduction, survey readability, and survey validation was employed. To develop and validate the Hospital for Special Surgery ACL Satisfaction Score (HSS ACL-SS), 70 patients were included in the survey development phase and 77 patients were included in the validation phase. HSS ACL-SS was compared to other currently used ACLR outcome measures including the International Knee Documentation Committee (IKDC) score, the Tegner/Lysholm score, and the Short-Form 12 Mental Component Score (SF-12 MCS) and Physical Component Score (SF-12 PCS). Test-retest reliability, internal consistency, convergent/discriminant validity, and floor/ceiling effects were assessed. RESULTS The HSS ACL-SS consists of 10 items identified by patiACLR. The results of this study show that the HSS ACL-ES may be a useful tool to measure post-operative patient satisfaction. Three species of the genus Allocreadium were collected from cyprinid fish in the territory of the Russian Far East Allocreadium khankaiensis sp.nov; Allocreadium hemibarbi Roitman, 1963, and Allocreadium sp. 1. It is established that, according to morphometric values, the species A. khankaiensis sp.nov. has high morphological similarity with Allocreadium elongatum Achmerov, 1960; Allocreadium erythroculteris Achmerov, 1960; and Allocreadium aburahaya Shimazu, 2003. However, A. khankaiensis sp.nov. differs from A. elongatum and A. FINO2 erythroculteris by vitellaria extension and size of cirrus pouch. In addition, A. khankaiensis sp.nov. differs from A. erythroculteris by the smaller size of the body and ventral sucker. A. khankaiensis sp.nov. and A. aburahaya have similar metrical values but A. khankaiensis sp.nov. differs by the shape of the testes rounded vs. lobed. Species affiliation of another Allocreadium species is based on the morphometric identity with the species A. hemibarbi. Phylogenetic tree reconstruction using partial sequences of the 28S rRNA gene revealed a clear resolution of all Allocreadium species, excluding Allocreadium lobatum and Allocreadium neotenicum. The genetic analysis detected Allocreadium sp. 1 as a presumably independent species from the Razdolnaya River basin.
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