Notes

Phage Number of Bicyclic Peptide Ligands of the Notch1 Receptor.
Recovery of the IAEA 2018 Proficiency Test Exercise water sample (n = 5) was 99% with an RSD of 11.9%. The method thus provides a powerful tool for the rapid analysis of low levels of 90Sr in various water/wastewater samples.This study described the first impedimetric immunosensor reported for the determination of CCR4, a new prostate cancer biomarker. This impedimetric immunosensor was constructed through the modification of disposable indium tin oxide (ITO) sheet with a conjugated pyrrole polymer P(Pyr-Pac) and subsequent immobilization of anti-CC chemokine receptor 4 (CCR4) antibodies. Acid-substituted poly(pyrrole) P(Pyr-Pac) polymer contained a lot of carboxyl groups on its end site, which were suitable for attachment of anti-CCR4 antibodies. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were chosen to investigate electrode preparation stages and, EIS was chosen to detect the CCR4 concentration. Anti-CCR4 antibody attached biosensing surface was highly selective to CCR4 antigen, the specific interaction resulted changes in electrochemical signal. Optimization studies containing polymer amount, anti-CCR4 antibody concentration, anti-CCR4 antibody immobilization time and anti-CCR4 antibody-CCR4 antigen interaction time were studied. The developed immunosensor displayed a linear increase with concentrations of CCR4 antigen (0.02-8 pg/mL) and a low detection limit of 6.4 fg/mL. In addition, this biosensor had great reproducibility and repeatability. Moreover, the designed biosensor was successfully used for the quantification of CCR4 antigen in serum samples. The recovery for the spiked serum samples was between 98.25% and 103.99%. The suggested immunosensor illustrated a good selectivity towards some interferents including different biomarkers. This study could establish a new approach for future cancer biomarker detection.Past several years have witnessed growing interest in developing wearable biosensors for non-invasive monitoring vital signs of chemical/biological markers such as lactate. In this context, textiles can be seen as a promising platform for the integration of wearable chemical sensors due to their inherent breathability, flexibility, softness and comfortableness. Gold is regarded as a preferred active sensing material due to its excellent biocompatibility, chemical inertness and wide electrochemical window. Here, a dry-spinning method was used to fabricate stretchable, strain-insensitive and highly conductive gold fibers. Such gold fibers could be used to fabricate lactate-sensing working electrodes, reference electrode, counter electrodes and further weaved into textiles in a standard three-electrode system with a planar layout. The textile lactate biosensors showed a high sensitivity of 19.13 μA/mM cm2 in phosphate-buffered solution (PBS) and 14.6 μA/mM cm2 in artificial sweat. This sensitivity could be maintained under high tensile strain up to 100% without external structural design. The results presented here indicate the potential application of wearable smart textile towards non-invasive lactate monitoring.Enzyme-linked immunosorbent assay is a widely used analytical technique for detecting and quantifying disease-specific protein biomarkers. Despite recent progresses in disease-specific protein biomarkers detection with microfluidic chips, many devices still suffer from the limited mass transport of target molecules, and consequently low detection efficiency or long incubation time. In this work, we present a novel strategy to significantly enhance the sensing efficiency of a chip-based biosensor by exploiting micro-streaming in an acoustofluidic device, which boosts intermolecular interactions and a hybridization chain reaction to increase the fluorescent signals. This device was made of a microfluidic chip that contains an array of PDMS micropillars in a ship-shaped microchannel. And the inner surface of the channel was functionalized with capture aptamers that bind with thrombin, chosen as a model target molecule. An ultrasonic transducer underneath the chip operating at 150 kHz generates circular micro-streaming flows around the pillars that significantly improves the binding efficiency of thrombin with capture aptamers by 1) increasing the retention time and 2) enhancing mass transport via local convection versus diffusion. The effects of ultrasound parameters, such as operating frequencies and voltages, on the distribution and magnitude of flows were optimized to obtain a better performance of the sensor chip. Under the optimized conditions, the detection limit was increased by one order of magnitude. Although this work has focused on the detection of thrombin as a model molecule, this streaming-enhanced, microstructure-based sensing strategy can be applied to detect a wide range of molecules or even cells.An ultrasensitive lateral flow immunoassay (LFIA) strip has been developed based on a multifunctional photothermal contrast Fe3O4@Au supraparticle (Fe3O4@Au SP) for quantitative Ochratoxin A (OTA) detection. The Fe3O4@Au SP composite not only shows a better photothermal effect over Fe3O4 and gold nanoparticles, but possesses magnetic property and excellent ability to directly adsorb protein (antibody). Under 808 nm irradiation, photothermal images of the test strips are recorded by a portable Infrared thermal camera. A quantitative analysis is easily achieved based on the thermal changes, which are proportional to the concentrations of analytes. Under an optimal condition, a wide linear detection range from 1 pg mL⁻1 to 1μgmL⁻1 and a limit of detection (LOD) at 0.12 pg mL⁻1 have been achieved with OTA as a model analytes. The practical application potential has also been validated by detection of OTA in spiked corn, peanut, and soybean extractives with overall recoveries ranging from 98.6% to 115% and coefficient of variations (CVs) between 6.06% and 12.73%. The photothermal LFIA renders a rapid, sensitive, and quantitative bio-/chemo-sensing platform with only a portable laser source and a thermal camera.Glycated hemoglobin (HbA1c) has been widely explored as an important marker for monitoring and diagnosing diabetes. Due to the advantages of high selectivity, easy preparation, and convenient preservation of aptamers, research on glycated hemoglobin detection utilizing aptasensors has received much attention in recent years. However, factors such as the pH and the salt concentration of the solution and the structure of the aptamer could influence the interactions between HbA1c and the aptamer. Benserazide mouse In this study, the factors were evaluated using surface plasmon resonance (SPR). The results show that the pH and the salt concentration can greatly affect the formation of a complex between the aptamer and HbA1c. In the stereostructure of the aptamer, loop L1 may be an important motif for recognizing glycated hemoglobin. In addition, the best condition for detecting HbA1c was at pH 6, with a high sensitivity and a low limit of detection(LOD) (1.06 × 10-3RUnM /2.55 nM). The results also demonstrated that the use of an SPR aptamer biosensor can be a sensitive technique to improve the accuracy and correctness of HbA1c measurement.
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