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Persistent Uveitis in Children.
Marine algae are abundant and inexhaustive sources of the bioactive compounds with the various benefits for human health. Among these substances an attention is given to the sulfated polysaccharides presented as the complexes of polymer macromolecules with excellent biological features including antioxidant, anti-inflammatory anticoagulant, antiviral, and immunomodulatory activities. In addition to the aforementioned properties there is a growing number of research results suggesting the bioactive sulfated polysaccharides such as carrageenan, fucoidan, laminarin, and others exert anticancer and antimetastatic properties. The present review contains the main results of experimental studies of the carrageenan anticancer activity including systemic and intracellular mechanisms of the antiproliferative influence. Relationships between structure, physico-chemical properties of carrageenan and their antitumor effects are described. There are data on the toxicology and pharmacokinetics of carrageenans as well as other aspects of their pharmacotherapeutic and pharmacoprophylactic influence that allow considering them as the potential anticancer agents.In our search for bioactive polysaccharides as immunomodulatory agents, an arabinofuranan (GMP90-1) was purified and characterized from the rinds of Garcinia mangostana L. GMP90-1 (absolute molecular weight 5.30 × 103 g/mol) was found to be composed of arabinose, galactose, and rhamnose. The backbone of GMP90-1 was determined as (1→5)-linked α-l-Araf, (1→2,3,5)-linked α-l-Araf, (1→3,5)-linked α-l-Araf, (1→6)-linked β-d-Galp, and (1→2)-linked α-l-Rhap. Conformational analysis revealed GMP90-1 to exist as a rigid rod structure in sodium chloride solution. To explore its potential as immunomodulatory agents, an in vitro cell screening was performed and GMP90-1 was found to significantly enhance the phagocytic uptake of neutral red and improve the secreted level of nitric oxide (NO), interleukin (IL)-6, IL-1β, and tumor necrosis factor-α (TNF-α) of macrophages. Furthermore, the cellular immunomodulatory activities were confirmed by the in vivo zebrafish experiment, which suggested that GMP90-1 with immunomodulatory effects could be considered as a potential immunomodulatory for immune diseases.Water-soluble chitosan (WSC) was used to enhance cadmium (Cd) phytoextraction by Hylotelephium spectabile, a promising accumulator. The effect of WSC on Cd forms and functional groups in contaminated soils was determined to clarify the internal mechanism of WSC affecting phytoremediation. Results showed that WSC significantly increased the water-soluble and acid-extractable Cd in contaminated soils by 20.3 %-143.5 % and 2.4 %-39.5 %, respectively. Fourier transform infrared spectra analysis showed that amino and hydroxyl groups in soils were significantly increased in the presence of WSC. The coordination of Cd with increased amino and hydroxyl groups may play important roles in formation of Cd complexes and mobilizing Cd in soils. WSC significantly increased the Cd phytoextraction of H. spectabile by 56.1 %-115.7 % without obvious growth inhibition, which was further confirmed under field trial conditions. Therefore, it is feasible to apply WSC as a soil amendment to Cd-contaminated soils during remediation with H. spectabile.Functional modifications of starch, such as paste properties, retrogradation, water absorption indexes, solubility, and swelling capacity, are induced by the amylose-lipid complex. This research comprehends the study of functional properties of extruded maize starch mixed with fatty acids (stearic acid, oleic acid, and maize oil) and the formation of amylose-lipid complexes. Maize starch with lipids (5 or 10 %), moisture (35 %) was extruded (single screw). Starch granule was modified by extrusion, to a lesser extent at 10 % of lipids, especially stearic acid, which covers starch granule surface. Viscosity decreased meaningfully with stearic acid addition. DSC showed both starch gelatinization enthalpy and amylose-lipid complex enthalpy for stearic or oleic acid, but it was just the first enthalpy for maize oil. X-ray diffraction showed orthorhombic crystals with or without the presence of lipids. Our results indicated that stearic acid yielded the highest amount of amylose-lipid complexes.Catalytic ionic liquid hydrolysis of cellulosic material have been considered as a green and highly efficient dissolution process. However, application of a pre-treatment process, i.e; ultrasonication enhances the hydrolysis of cellulose in ionic liquid by providing mechanical force. In this paper, we describe the impact of both chemical and mechanical approaches to produce nanocrytalline cellulose (NCC) with anticipated particle size, and crystallinity with improved yields. The ultrasonication treatment was evaluated in terms of treatment time and vibration amplitude. It was found that the lowest ultrasonication time (5 min) produced the NCC of highest crystallinity (73 %), but the lowest yield (84 %). In contrary, the highest ultrasonication vibration amplitude at 90 % produced NCC with highest crystallinity value (67 %) as well as yields (90 %). It concludes that ultrasonic pre-treatment improves the hydrolysis process of cellulose in ionic liquid with increasing yield and crystallinity of NCC.Chinese water chestnut peels are a kind of vegetable processing waste containing many active components such as polysaccharides, the structure of which remains unknown. To elucidate the structure of polysaccharides from Chinese water chestnut peels, two polysaccharides named WVP-1 and WVP-2 were isolated. WVP-1 (3.16 kDa) consisted of mannose (1.75 %), glucose (84.69 %), galactose (6.32 %), and arabinose (7.24 %), while WVP-2 (56.97 kDa) was composed of mannose (3.18 %), rhamnose (1.52 %), glucuronic acid (1.42 %), galacturonic acid (4.83 %), glucose (11.51 %), galactose (36.02 %), and arabinose (41.53 %). Linkage and NMR data indicated that WVP-1 was composed mainly of →4)-α-d-Glcp(1→ and a certain proportion of →3)-β-d-Glcp-(1→, including linear and branched polysaccharides simultaneously. WVP-2 was a pectin-like polysaccharide with →4)-α-d-GalpA6Me-(1→ units and the branch points of →3,4)-α-l-Arap-(1→, →3,6)-β-d-Galp-(1→. WVP-2 exhibited stronger potential antioxidant and immunomodulatory activities than WVP-1 in vitro. These results provide a foundation for the further study of polysaccharides from Chinese water chestnut peels.Lignin-containing cellulose nanofibers (LCNFs) from energy cane bagasse (ECB), were prepared using microwave assisted deep eutectic solvent (MV-DES) treatment in combination with ultrasonication. The yield of lignocellulose is up to 45.2 % with 81.0 % delignification under the optimal reaction condition (110 ℃, 30 min). The resulting LCNF exhibited a highly entangled network, which was caused by the binder role of lignin between cellulose nanofibers. The addition of LCNFs improved the stability of the polyanionic cellulose (PAC) film-forming suspension, which was confirmed by the increased zeta potential and viscosity values. Mocetinostat concentration The LCNF / PAC films showed tunable mechanical and UV-resistant properties, depending on the amount and type of LCNFs. PAC films with the addition of 5 % LCNFs (PEF-5 %) showed good mechanical properties (a tensile strength of 55.8 MPa with a 26.3 % strain to break) and high UV protection ability (a UV-transmittance of 2.9 %).Selenium oxychloride (SOC) was employed as a highly reactive selenide reagent to synthesize selenized Artemisia sphaerocephala polysaccharides (SeASP). Se content of SeASP was significantly increased (∼22,400 μg/g) as compared to HNO3/H2SeO3 selenylation method (1703 μg/g). Furthermore, selenized ASP was prepared by using microwave-assisted synthesis which obviously enhanced selenylation kinetics. FT-IR, Raman, XPS and NMR results exhibited seleno-group was substituted at C6 position in the form of selenite (Se4+). SEC-MALLS suggested SOC system could effectively avoid the degradation of polysaccharide chain. Meanwhile, MALLS calculation, MB spectrophotometric method and AFM observation showed SeASP appeared spherical and rod-shaped conformation after selenylation. Seleno-groups were more likely to affect the conformational transformation of polysaccharide chains. Moreover, SeASP could significantly enhance antiproliferative activity against three tumor cells, of which the IC50 value of HepG2 was calculated as 24.35 μg/mL. It was found that higher Se content could effectively improve the antitumor activities of Se-polysaccharides in vitro.Structurally different polymers were derived from Picea abies foliage by successive extraction with water (PAW), HCl solution (PAA) and (NH4)2C2O4 solution (PAO). The P. abies foliage was found to contain basically low-methoxyl pectin extractable with an (NH4)2C2O4 solution. PAW was shown to comprise primarily arabinogalactan-proteins (AGPs); PAA was composed of mixed AGPs and pectic polysaccharides, with the latter prevailing; and polysaccharide PAO isolated in the highest yield included chiefly pectic polysaccharides. The major constituents of PAO were low-methoxyl and low-acetylated 1,4-α-d-galacturonan and partially acetylated RG-I. The sugar side chains of RG-I contained chiefly highly branched 1,5-α-l-arabinan and arabinogalactan type I as a minor constituent. RG-I whose side chains had 1,5-α-l-arabinan represented short regions alternating with non-acetylated and unmethylesterified galacturonan regions. In addition to pectins, polysaccharide PAO contained AGPs, xylanes and glucomannans, indicating that these polysaccharides are in an intimate interaction.The heterogeneous sulfoethylation of cellulose, xylan, α-1,3-glucan, glucomannan, pullulan, curdlan, galactoglucomannan, and agarose was studied using sodium vinylsulfonate (NaVS) as reagent in presence of sodium hydroxide and iso-propanol (i-PrOH) as slurry medium. The influence of the concentration of polymer, water, and NaOH (solid or aqueous solution) on the degree of substitution (DS) was investigated. The sulfoethylation rendered the polysaccharides studied water-soluble. Sulfoethylation of heteropolysaccharides yielded products with higher DS compared to the conversion of homopolysaccharides. Structure characterization was carried out by means of 13C-NMR spectroscopy.In this study, LPIIa, a purified polysaccharide from longan pulp, was isolated. Its anti-inflammatory activity and intestinal barrier protection were investigated with LPS-treated co-culture model of Caco-2 cells and RAW 264.7 macrophages. The average molecular weight LPIIa was 159.3 kDa. Its detailed structure was shown below. The backbone of LPIIa was composed of (1→3,4)-linked-α-Rhap, (1→4)-linked-β-Galp, (1→6)-linked-β-Galp, and (1→3,6)-linked-β-Galp, with branches at the O-4 of Rha and O-3 of Gal, consisting of side chains of α-Araf, β-Galp, and α-Glcp. In LPS-induced RAW 264.7 macrophages, LPIIa suppressed the production of inflammatory mediators, including TNF-α, IL-6, NO, and PGE2, and inhibited iNOS and COX-2 gene expression. In addition, LPIIa attenuated intestinal tight-junctional channel protein Claudin-2 expression and increased tight-junctional barrier protein ZO-1 expression in Caco-2 cells. Knowing the structural features and activities of longan polysaccharide gives insights into longan polysaccharide application as an anti-inflammatory agent or adjuvant in curing the intestinal inflammation.
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