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In this study, five spatially balanced sampling methods, i.e., generalized random-tessellation stratified (GRTS), local pivotal method (LPM), spatially correlated Poisson sampling (SCPS), local cube method (LCUBE), and balanced acceptance sampling (BAS) were compared to simple random sampling (SRS) based on a livestock disease transmission model on a hypothetical region (195 km × 300 km) populated with 6000 farms in terms of the probability of detection by sample size. Given a fixed sample size, four of the five spatially balanced sampling methods provided better performance than SRS, i.e., higher probabilities of detecting at least one infected farms over a range of regional prevalence evaluated (1%-5%). That is, for any given probability of detection, spatially balanced methods required testing fewer farms than SRS. In an era of pandemics, active regional surveillance for early detection of emerging pathogens becomes urgent, yet shrinking budgets impose intractable constraints. The better performance and higher efficiency of spatially balanced sampling methods suggests a potential improvement in regional livestock disease surveillances and a partial solution to the challenge of affordable surveillance.The monitoring of the disease prevalence in a population is an essential component of its adaptive management. However, field data often lead to biased estimates. This is the case for brucellosis infection of ibex in the Bargy massif (France). A test-and-cull program is being carried out in this area to manage the infection captured animals are euthanized when seropositive, and marked and released when seronegative. Because this mountainous species is difficult to capture, field workers tend to focus the capture effort on unmarked animals. Indeed, marked animals are less likely to be infected, as they were controlled and negative during previous years. As the proportion of marked animals in the population becomes large, captured animals can no longer be considered as an unbiased sample of the population. Cyclopamine nmr We designed an integrated Bayesian model to correct this bias, by estimating the seroprevalence in the population as the combination of the separate estimates of the seroprevalence among unmarked animals (estimated from the data) and marked animals (estimated with a catalytic infection model, to circumvent the scarcity of the data). As seroprevalence may not be the most responsive parameter to management actions, we also estimated the proportion of animals in the population with an active bacterial infection. The actual infection status of captured animals was thus inferred as a function of their age and their level of antibodies, using a model based on bacterial cultures carried out for a sample of animals. Focusing on the population of adult females in the core area of the massif, i.e. with the highest seroprevalence, this observational study shows that seroprevalence has been divided by two between 2013 (51%) and 2018 (21%). Moreover, the likely estimated proportion of actively infected females in the same population, though very imprecise, has decreased from a likely estimate of 34% to less than 15%, suggesting that the management actions have been effective in reducing infection prevalence.Foaming is a common operational problem that occurs in activated sludge (AS) from many wastewater treatment plants (WWTPs), but the characteristic of antibiotic resistance genes (ARGs) and human pathogenic bacteria (HPB) in foams is generally lacking. Here, we used a metagenomic approach to characterize the profile of ARGs and HPB in foams and AS from full-scale WWTPs receiving pesticide wastewater. No significant difference in the microbial communities was noted between the AS and foam samples. The diversity and abundance of ARGs in the foams were similar to those in the pertinent AS samples. Procrustes analysis suggested that the bacterial community is the major driver of ARGs. Metagenomic assembly also indicated that most ARGs (e.g., multidrug, rifamycin, peptides, macrolide-lincosamide-streptogramin, tetracycline, fluoroquinolone, and beta-lactam resistance genes) were carried by chromosomes rather than mobile genetic elements. Moreover, the relative abundances of HPB, Pseudomonas putida and Mycobacterium smegmatis, were enriched in the foam samples. Nine HPB were identified as carriers of 21 ARG subtypes, of which Pseudomonas aeruginosa could carry 12 ARG subtypes. Overall, this study indicates the prevalence of ARGs, HPB, and ARG-carrying HPB in foams, which highlights the potential risk of foams in spreading ARGs and HPB into the surrounding environments.Removal of lead (II) (Pb2+) from contaminated soil was investigated using a new-type switchable-array-electrode electrokinetics assisted by permeable reactive barrier (SAE/EK-PRB). The soil pH after treatments with SAE/EK-PRB ranged from 3.5 to 4.8, and the peak values of current density was highest with 3.01 mA/cm2 at voltage gradient 2.5 V/cm. Both voltage gradient and moisture content promoted the removal of Pb2+. Moisture content 35% and 2.5 V/cm were considered as the optional operating conditions. Different PRB filling materials including zeolite, fly ash, active carbon alone and the combination of fly ash and graphene oxide were, therefore, investigated under the optional operating conditions. The highest removal reached 92.6% with the combination of fly ash and graphene oxide. Pb2+ forms in the soil with different PRB filling materials showed the percentages of weak bindings, such as exchangeable and carbonate forms decreased. However, strong binding, residual form increased largely, indicating the SAE/EK-PRB could effectively reduce the environmental risk of Pb2+ to a great extent. Fly ash and the combination of zeolite and fly ash as PRB filling materials demonstrated to be highly promising for the effective EK remediation of Pb2+-contaminated soil, taking the economic and environmental principles and remediation efficiency into consideration.Lead pollution precaution caused by lead-based anode corrosion is a hot and challenging issue for zinc electrowinning. A novel functional lead-based anode (MnO2 pre-coated anode-MPA) was precisely fabricated and its long-term performances were studied compared with typical Pb-1%Ag anode (TPA). Results indicated that MPA posed excellent effects on synergistic inhibiting lead dissolution and reducing hazardous pollutants generation, and decreasing the lead content of zinc products by 81%. Further, the underlying mechanism of film growth and transformation in structure, composition and crystal phase, the migration and distribution of lead and anode slime during electrolytic, were clarified in-depth. Dynamic material flow analysis confirmed that MPA reduced the entire lead migration amount by over 92% compared with TPA. The compact multilayer structure of the MPA film and self-reparation effects of local structure provided better and persistent protection for the lead matrix, which greatly retarded the high-speed corrosion of lead anode.
Website: https://www.selleckchem.com/products/Cyclopamine.html
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