Notes

Improved degradation involving 4-aminobenzenesulfonate by way of a co-culture associated with Afipia sp. 624S and also Diaphorobacter sp. 624L.
Variation among species and datasets highlights difficulties in identifying all HT, but our investigations showed that sample sequence analyses can reveal the importance of HT for the diversification of the TE repertoire of plants.
In the Netherlands, the bivalent HPV vaccine (2vHPV) has been offered to preadolescent girls via the National Immunization Program (NIP) in a two-dose (2D) schedule since 2014. The current study estimates vaccine effectiveness (VE) against HPV infections up to four years post-vaccination among girls eligible for routine 2D immunization.

A cohort study (HAVANA2) was used in which participants annually filled out an online questionnaire and provided a vaginal self-sample for determination of HPV by the SPF10-LiPA25 assay, able to detect 25 HPV types. VE against incident type-specific infections and pooled outcomes was estimated by a Cox proportional hazards model with shared frailty between the HPV types.

In total, 2027 girls were included in the study, 1098 (54.2%) of whom were vaccinated with two doses. Ruboxistaurin Highest incidence rate was 5.0/1000 person-years (HPV51) among vaccinated participants and 9.1/1000 person-years (HPV74) among unvaccinated participants. Adjusted pooled VE was 84.0% (95%CI, 27.0-96.5%) against incident HPV16/18 infections and 86.5% (95%CI, 39.5-97.08%) against cross-protective types HPV31/33/45.

Four years post-vaccination, two doses of 2vHPV vaccination were effective in the prevention of incident HPV16/18 infections and provided cross-protection to HPV31/33/45. Our VE estimates rival those from three-dose schedules, indicating comparable protection by 2D schedules.
Four years post-vaccination, two doses of 2vHPV vaccination were effective in the prevention of incident HPV16/18 infections and provided cross-protection to HPV31/33/45. Our VE estimates rival those from three-dose schedules, indicating comparable protection by 2D schedules.The desire for in vitro genotoxicity assays to provide higher information content, especially regarding chemicals' predominant genotoxic mode of action, has led to the development of a novel multiplexed assay available under the trade name MultiFlow®. We report here on an experimental design variation that provides further insight into clastogens' genotoxic activity. First, the standard MultiFlow DNA Damage Assay-p53, γ H2AX, phospho-histone H3 was used with human TK6 lymphoblastoid cells that were exposed for 24 continuous hours to each of 50 reference clastogens. This initial analysis correctly identified 48/50 compounds as clastogenic. These 48 compounds were then evaluated using a short-term, 'pulse' treatment protocol whereby cells were exposed to test chemical for 4 h, a centrifugation/washout step was performed, and cells were allowed to recover for 20 h. MultiFlow analyses were accomplished at 4 and 24 h. The γ H2AX and phospho-histone H3 biomarkers were found to exhibit distinct differences in terms of their persistence across chemical classes. Unsupervised hierarchical clustering analysis identified three groups. Examination of the compounds within these groups showed one cluster primarily consisting of alkylators that directly target DNA. The other two groups were dominated by non-DNA alkylators and included anti-metabolites, oxidative stress inducers and chemicals that inhibit DNA-processing enzymes. These results are encouraging, as they suggest that a simple follow-up test for in vitro clastogens provides mechanistic insights into their genotoxic activity. This type of information will contribute to improve decision-making and help guide further testing.
Methionine sulfoxidation is a post-translational modification playing important roles in cell signaling. Herein, we present ptm, an R package for the study of this modification. However, since many of the analyses applied to methionine modification can be extended to other modifications, the package can be useful to thoroughly analyze post-translational modifications in general. Thus, within a single software environment ptm can integrate information from up to 11 databases covering 9 modifications. Different functions can work coordinately to form pipelines allowing the programmatic analysis of thousands of proteins. Alternatively, the user can simultaneously perform different analyses on the same protein of interest, combining the results in a single output. The flexibility of ptm makes it a suitable tool to address site- and protein-centric hypotheses related to post-translational modifications. Accompanying the package we maintain a web page containing extended documentation and examples of the tasks that can be performed with ptm.

ptm is implemented in R. Release versions are available via CRAN and work on all major operating systems. The development version is maintained at https//bitbucket.org/jcaledo/ptm. Extended documentation can be found at https//metositeptm.com.

Supplementary data are available at Bioinformatics online.
Supplementary data are available at Bioinformatics online.
During the COVID-19 pandemic, stigmatization of older persons has increased in traditional and social media. It was unknown whether this negative messaging could be detrimental to the mental health of older individuals, and whether the relatively uncommon positive messaging about older individuals could benefit their mental health.

To address these gaps, we designed age-stereotype interventions based on actual news stories that appeared during the pandemic, and divided them into negative and positive versions of what we term personified (i.e., person-based) and enumerative (i.e., number-based) age-stereotype messaging. The negative versions of the two types of messaging reflected the age stereotype of decline whereas the positive versions of the two types of messaging reflected the age stereotype of resilience.

As expected, the exposure of older individuals to the negative-age-stereotype-messaging interventions led to significantly worse mental health (more anxiety and less peacefulness), compared to a
Maintaining cognitive function is an important component of healthy aging. There is increasing recognition that extraneous factors expedite the typical cognitive aging process. Risk factors for cognitive decline cluster around inequalities and disproportionally affect minority and vulnerable groups. Taking a minority stress approach, we examined the relationship between proxy measures of minority stress and cognitive health in a large sample of Canadians aged 45-85 years.

Data were drawn from the baseline of the Canadian Longitudinal Study on Aging (CLSA), a prospective cohort study. Memory (n = 36,849) and executive function (n = 36,266) were assessed using standardized assessment tools. We ran multiple linear regression models with memory and executive function as the outcomes. Explanatory variables included known correlates of cognitive health (i.e., demographic, health, and cognitive reserve) and proxy measures of minority stress (i.e., sexual orientation, race, and perceived social standing).

Results were consistent with existing evidence showing that demographic and health variables were associated with cognitive performance.
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